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Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds
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The authors report an efficient screen for mutants that secrete in seeds a storage vacuole marker, SP-GFP-CT24, which consists on GFP with a signal peptide fused to the vacuolar sorting determinant from β-conglycinin. Secretion of SP-GFP-CT24 stabilizes the protein and the mutant seeds are highly fluorescent. Through this screen the authors have found novel alleles of VSR1 and a membrane-associated protein of unknown function.
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Fuji K., Shimada T., Takahashi H., Tamura K., Koumoto Y., Utsumi S., Nishizawa K., Maruyama N., and Hara-Nishimura I. Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds. Plant Cell 19 (2007) 597-609. The authors report an efficient screen for mutants that secrete in seeds a storage vacuole marker, SP-GFP-CT24, which consists on GFP with a signal peptide fused to the vacuolar sorting determinant from β-conglycinin. Secretion of SP-GFP-CT24 stabilizes the protein and the mutant seeds are highly fluorescent. Through this screen the authors have found novel alleles of VSR1 and a membrane-associated protein of unknown function.
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The dynamic changes of tonoplasts in guard cells are important for stomatal movement in Vicia faba
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Gao X.Q., Li C.G., Wei P.C., Zhang X.Y., Chen J., and Wang X.C. The dynamic changes of tonoplasts in guard cells are important for stomatal movement in Vicia faba. Plant Physiol 139 (2005) 1207-1216
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Higaki T., Goh T., Hayashi T., Kutsuna N., Kadota Y., Hasezawa S., Sano T., and Kuchitsu K. Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: in vivo imaging of hypersensitive cell death in tobacco BY-2 cells. Plant Cell Physiol 48 (2007) 1414-1425
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Olbrich A., Hillmer S., Hinz G., Oliviusson P., and Robinson D.G. Newly formed vacuoles in root meristems of barley and pea seedlings have characteristics of both protein storage and lytic vacuoles. Plant Physiol 145 (2007) 1383-1394
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Otegui M.S., Noh Y.S., Martinez D.E., Vila Petroff M.G., Staehelin L.A., Amasino R.M., and Guiamet J.J. Senescence-associated vacuoles with intense proteolytic activity develop in leaves of Arabidopsis and soybean. Plant J 41 (2005) 831-844
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Happel N., Honing S., Neuhaus J.M., Paris N., Robinson D.G., and Holstein S.E. Arabidopsis mu A-adaptin interacts with the tyrosine motif of the vacuolar sorting receptor VSR-PS1. Plant J 37 (2004) 678-693
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The PA-TM-RING protein RING finger protein 13 is an endosomal integral membrane E3 ubiquitin ligase whose RING finger domain is released to the cytoplasm by proteolysis
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Bocock J.P., Carmicle S., Chhotani S., Ruffolo M.R., Chu H., and Erickson A.H. The PA-TM-RING protein RING finger protein 13 is an endosomal integral membrane E3 ubiquitin ligase whose RING finger domain is released to the cytoplasm by proteolysis. FEBS J 276 (2009) 1860-1877
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Surpin M., Rojas-Pierce M., Carter C., Hicks G.R., Vasquez J., and Raikhel N.V. The power of chemical genomics to study the link between endomembrane system components and the gravitropic response. Proc Natl Acad Sci U S A 102 (2005) 4902-4907
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Zouhar J., Hicks G.R., and Raikhel N.V. Sorting inhibitors (Sortins): chemical compounds to study vacuolar sorting in Arabidopsis. Proc Natl Acad Sci U S A 101 (2004) 9497-9501
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Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1
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Robert S., Chary S.N., Drakakaki G., Li S., Yang Z., Raikhel N.V., and Hicks G.R. Endosidin1 defines a compartment involved in endocytosis of the brassinosteroid receptor BRI1 and the auxin transporters PIN2 and AUX1. Proc Natl Acad Sci U S A 105 (2008) 8464-8469
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54
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Arabidopsis P-glycoprotein19 participates in the inhibition of gravitropism by gravacin
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This work illustrates an importance of Arabidopsis as a model organism in both classical and chemical genetics. Using a mutant collection, an auxin transporter was identified as a target of a potent gravitropism inhibitor.
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Rojas-Pierce M., Titapiwatanakun B., Sohn E.J., Fang F., Larive C.K., Blakeslee J., Cheng Y., Cutler S.R., Peer W.A., Murphy A.S., et al. Arabidopsis P-glycoprotein19 participates in the inhibition of gravitropism by gravacin. Chem Biol 14 (2007) 1366-1376. This work illustrates an importance of Arabidopsis as a model organism in both classical and chemical genetics. Using a mutant collection, an auxin transporter was identified as a target of a potent gravitropism inhibitor.
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Identification of cellular pathways affected by Sortin2, a synthetic compound that affects protein targeting to the vacuole in Saccharomyces cerevisiae
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Norambuena L., Zouhar J., Hicks G.R., and Raikhel N.V. Identification of cellular pathways affected by Sortin2, a synthetic compound that affects protein targeting to the vacuole in Saccharomyces cerevisiae. BMC Chem Biol 8 (2008) 1
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