Specimen thickness dependence of hydrogen evolution during cryo-transmission electron microscopy of hydrated soft materials

Journal of Microscopy

Volumn 236, Issue 3, 2009, Pages 174-179

  Yakovlev, S ^a   Misra, M ^b   Shi, S ^b   Libera, M ^a  

^a STEVENS INSTITUTE OF TECHNOLOGY   (United States)

^b UNILEVER RESEARCH   (United Kingdom)

Author keywords

Cryo TEM; EELS; Electron energy loss spectroscopy; Nafion; Scanning transmission electron microscopy; Skin; STEM; Water mapping

Indexed keywords

DISSOCIATION; ELECTRON EMISSION; ELECTRON ENERGY LEVELS; ELECTRON IRRADIATION; ELECTRON SCATTERING; ELECTRONS; ENERGY DISSIPATION; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; HYDRATION; SCANNING ELECTRON MICROSCOPY; SPECTROSCOPIC ANALYSIS; SPECTRUM ANALYSIS;

CRYO-TEM; ELECTRON ENERGY-LOSS SPECTROSCOPIES; EVOLUTION OF HYDROGENS; NAFIONS; SCANNING TRANSMISSION ELECTRON MICROSCOPY; SOFTER MATERIALS; SPECIMEN THICKNESS; TRANSMISSION ELECTRON; WATER MAPPING;

ELECTRON ENERGY LOSS SPECTROSCOPY;

HYDROGEN; ORGANIC SOLVENT; PERFLUORO COMPOUND;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; ARTIFACT; CRYOPRESERVATION; DIFFUSION; ELECTRON ENERGY LOSS SPECTROSCOPY; HYDRATION; IRRADIATION; MATERIALS; NONHUMAN; PRIORITY JOURNAL; SURFACE PROPERTY; SWINE; THICKNESS; TRANSMISSION ELECTRON MICROSCOPY;

SUS;

EID: 70450207195     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03215.x     Document Type: Article

References (17)

1. Dubochet, J., Chang, J.J. Freeman, R. (1982) Frozen aqueous suspensions. Ultramicroscopy 10, 55 62.
2. Dubochet, J., Adrian, M., Chang, J.-J., Homo, J.-C., Lepault, J., McDowell, A. Schultz, P. (1988) Cryo-electron microscopy of vitrified specimens. Qtr. Rev. Biophys. 21, 129 228.
3. Egerton, R.F. (1996) Electron Energy Loss Spectroscopy in the Electron Microscope. Plenum, New York.
4. Erni, R., Browning, N., Dai, Z.R. Bradley, J. (2005) Analysis of extraterrestrial particles using monochromated electron energy-loss spectroscopy. Micron 36, 369 379. (Pubitemid 40588994)
5. Heitner-Wirguin, C. (1996) Recent advances in perfluorinated ionomer membranes: structure, properties and applications. J. Membr. Sci. 120, 1 33.
6. Leapman, R.D. Sun, S. (1995) Cryo-electron energy loss spectroscopy: observations on vitrified hydrated specimens and radiation damage. Ultramicroscopy 59, 71 79.
7. Leapman, R.D. Swyt, C.R. (1988) Separation of overlapping core edges in electron energy loss spectra by multiple-least-squares fitting. Ultramicroscopy 26, 393 403.
8. Malis, T., Cheng, S.C. Egerton, R.F. (1988) EELS log-ratio technique for specimen-thickness measurement in the TEM. J. Electron Microsc. Tech. 8, 193 200.
9. Markland, T.E., Habershon, S. Manolopoulos, D.E. (2008) Quantum diffusion of hydrogen and muonium atoms in liquid water and hexagonal ice. J. Chem. Phys. 128, 194506.
10. Mauritz, K.A. Moore, R.B. (2004) State of understanding of Nafion. Chem. Rev. 104, 4535 4585.
11. Rivin, D., Kendrick, C.E., Gibson, P.W. Schneider, N.S. (2001) Solubility and transport behavior of water and alcohols in Nafion. Polymer 42, 623 635.
12. Schneider, N.S. Rivin, D. (2006) Solvent transport in hydrocarbon and perfluorocarbon ionomers. Polymer 47, 3119 3131.
13. Sousa, A., Aitouchen, A. Libera, M. (2006) Water mapping in hydrated soft materials. Ultramicroscopy 106, 130 145.
14. Sun, S., Shi, S. Leapman, R.D. (1993) Water distributions of hydrated biological specimens by valence electron energy loss spectroscopy. Ultramicroscopy 50, 127 139.
15. Sun, S.Q., Shi, S.-L., Hunt, J.A. Leapman, R.D. (1995) Quantitative water mapping of cryosectioned cells by electron energy-loss spectroscopy. J. Microsc. 177, 18 30.
16. Yakovlev, S. Libera, M. (2008) Dose-limited spectroscopic imaging of soft materials by low-loss EELS in the scanning transmission electron microscope. Micron 39, 734 740.
17. Zheng, W., Jewitt, D. Kaiser, R.I. (2006) Formation of hydrogen, oxygen, and hydrogen peroxide in electron-irradiated crystalline water ice. Astrophys. J. 639, 534 548.