Comparison of chemical binding to recombinant fathead minnow and human estrogen receptors alpha in whole cell and cell-free binding assays

Environmental Toxicology and Chemistry

Volumn 28, Issue 10, 2009, Pages 2175-2181

  Rider, Cynthia V ^a,b   Hartig, Phillip C ^b   Cardon, Mary C ^b   Wilson, Vickie S ^b  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Competitive binding; In vitro; Saturation binding; Xenoestrogens

Indexed keywords

AMINO ACID SEQUENCE; ASSAY SYSTEM; BINDING ASSAYS; CADMIUM CHLORIDE; CELL-FREE; CELL-FREE ASSAYS; CHEMICAL BINDING; COMPETITIVE BINDING; COMPETITIVE BINDING ASSAY; DI-BUTYL PHTHALATE; ELEVATED TEMPERATURE; ENDOCRINE DISRUPTING CHEMICALS; ESTROGEN RECEPTOR; ETHINYLESTRADIOL; FATHEAD MINNOW; HUMAN ESTROGEN RECEPTORS; IN-VITRO; IN-VITRO ASSAYS; PHTHALATES; SPECIFIC BINDING; WHOLE CELL; XENOESTROGENS;

AMINES; AMINO ACIDS; BINDERS; BINDING SITES; CADMIUM; CELLS; CHEMICALS; CHLORINE COMPOUNDS; ESTERS; MAMMALS; MICROARRAYS; ORGANIC ACIDS;

ASSAYS;

ALPHA ZEARALENOL; BACULOVIRUS VECTOR; CADMIUM CHLORIDE; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; ETHINYLESTRADIOL; FULVESTRANT; PHTHALIC ACID BENZYL BUTYL ESTER; PHTHALIC ACID DIBUTYL ESTER;

AMINO ACID; BIOASSAY; CHEMICAL BINDING; COMPARATIVE STUDY; CYPRINID; ENDOCRINE DISRUPTOR; ESTROGENIC COMPOUND; RECOMBINATION;

ARTICLE; BINDING ASSAY; CELL FREE SYSTEM; CHEMICAL BINDING; COMPARATIVE STUDY; GENETIC TRANSFECTION; HUMAN; IC 50; IN VITRO STUDY; NONHUMAN; PIMEPHALES PROMELAS; PRIORITY JOURNAL; WHOLE CELL;

ANIMALS; BINDING SITES; BINDING, COMPETITIVE; CADMIUM CHLORIDE; CELL-FREE SYSTEM; CERCOPITHECUS AETHIOPS; COS CELLS; CYPRINIDAE; DIBUTYL PHTHALATE; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; ETHINYL ESTRADIOL; HUMANS; PHTHALIC ACIDS; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE; ZERANOL;

MAMMALIA; PIMEPHALES PROMELAS; PISCES;

EID: 70449905627     PISSN: 07307268     EISSN: None     Source Type: Journal    
DOI: 10.1897/09-018.1     Document Type: Article

References (30)

1. Jobling S, Nolan M, Tyler CR, Brighty G, Sumpter JP. 1998. Widespread sexual disruption in wild fish. Environ Sci Technol 32: 2498-2506. (Pubitemid 28420276)
2. Desbrow C, Routledge EJ, Brighty GC, Sumpter JP, Waldock M. 1998. Identification of estrogenic chemicals in STW effluent. I. Chemical fractionation and in vitro biological screening. Environ Sci Technol 32:1549-1558.
3. Pakdel F, Reese JC, Katzenellenbogen BS. 1993. Identification of charged residues in an N-terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol Endocrinol 7:1408-1417. (Pubitemid 23345016)
4. Komagata S, Nakajima M, Tsuchiya Y, Katoh M, Kizu R, Kyo S, Yokoi T. 2006. Decreased responsiveness of naturally occurring mutants of human estrogen receptor alpha to estrogens and antiestrogens. J Steroid Biochem Mol Biol 100:79-86. (Pubitemid 44088613)
5. Petit F, Valotaire Y, Pakdel F. 1995. Differential functional activities of rainbow-trout and human estrogen receptors expressed in the yeast Saccharomyces cerevisiae. Eur J Biochem 233: 584-592.
6. Tan NS, Frecer V, Lam TJ, Ding L. 1999. Temperature dependence of estrogen binding: Importance of a subzone in the ligand binding domain of a novel piscine estrogen receptor. Biochim Biophys Acta Mol Cell Res 1452:103-120. (Pubitemid 29528258)
7. Sumida K, Ooe N, Saito K, Kaneko H. 2003. Limited species differences in estrogen receptor alpha-medicated reporter gene transactivation by xenoestrogens. J Steroid Biochem Mol Biol 84: 33-40. (Pubitemid 36324435)
8. Matthews J, Celius T, Halgren R, Zacharewski T. 2000. Differential estrogen receptor binding of estrogenic substances: A species comparison. J Steroid Biochem Mol Biol 74:223-234. (Pubitemid 32121658)
9. Olsen CA, Meussen-Elholm ETM, Hongslo JK, Stenersen J, Tollefsen KE. 2005. Estrogenic effects of environmental chemicals: An interspecies comparison. Comp Biochem Physiol C, Toxicol Pharmacol 141:267-274. (Pubitemid 41206681)
10. Matthews JB, Fertuck KC, Celius T, Huang YW, Fong CJ, Zacharewski TR. 2002. Ability of structurally diverse natural products and synthetic chemicals to induce gene expression mediated by estrogen receptors from various species. J Steroid Biochem Mol Biol 82:181-194. (Pubitemid 35435366)
11. Matthews J, Zacharewski T. 2000. Differential binding affinities of PCBs, HO-PCBs, and aroclors with recombinant human, rainbow trout (Onchorhynkiss mykiss), and green anole (Anolis carolinensis) estrogen receptors, using a semi-high throughput competitive binding assay. Toxicol Sci 53:326-339. (Pubitemid 30100150)
12. Ledrean Y, Kern L, Pakdel F, Valotaire Y. 1995. Rainbow-trout estrogen receptor presents an equal specificity but a differential sensitivity for estrogens than human estrogen receptor. Mol Cell Endocrinol 109:27-35.
13. Vonier PM, Crain DA, McLachlan JA, Guillette LJ, Arnold SF. 1996. Interaction of environmental chemicals with the estrogen and progesterone receptors from the oviduct of the American alligator. Environ Health Perspect 104:1318-1322.
14. Rider CV, Hartig PC, Cardon MC, Wilson VS. 2009. Development of a competitive binding assay with recombinant estrogen receptors from multiple species. Toxicol Lett 184:85-89.
15. Wilson VS, Cardon MC, Thornton J, Korte JJ, Ankley GT, Welch J, Gray LE, Hartig PC. 2004. Cloning and in vitro expression and characterization of the androgen receptor and isolation of estrogen receptor alpha from the fathead minnow (Pimephales promelas). Environ Sci Technol 38:6314-6321. (Pubitemid 39578645)
16. Pakdel F, Le Gac F, Le Goff P, Valotaire Y. 1990. Full-length sequence and in vitro expression of rainbow trout estrogen receptor cDNA. Mol Cell Endocrinol 71:195-204. (Pubitemid 20221454)
17. Klinge CM. 1999. Role of estrogen receptor ligand and estrogen response element sequence on interaction with chicken ovalbumin upstream promoter transcription factor (COUP-TF). J Steroid Biochem Mol Biol 71:1-19.
18. Summers MD, Smith GE. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agriculture Experiment Station Bulletin 1555. Texas A&M University, College Station, TX, USA.
19. O'Reilly DR, Miller LK, Luckow VA. 1992. Baculovirus Expression Vectors: A Laboratory Manual. WH Freeman, New York, NY, USA.
20. Matthews JB, Clemons JH, Zacharewski TR. 2001. Reciprocal mutagenesis between human alpha (L349, M528) and rainbow trout (M317, 1496) estrogen receptor residues demonstrates their importance in ligand binding and gene expression at different temperatures. Mol Cell Endocrinol 183:127-139.
21. Wong CI, Kelce WR, Sar M, Wilson EM. 1995. Androgen receptor antagonist versus agonist activities of the fungicide vinclozolin relative to hydroxyflutamide. J Biol Chem 270: 19998-20003.
22. Williams D, Gorski J. 1974. Equilibrium binding of estradiol by uterine cell suspensions and whole uteri in vitro. Biochemistry (Mosc) 13:5537-5542.
23. Brzozowski AM, Pike ACW, Dauter Z, Hubbard RE, Bonn T, Engstrom O, Ohman L, Greene GL, Gustafsson JA, Carlquist M. 1997. Molecular basis of agonism and antagonism in the oestrogen receptor. Nature 389:753-758. (Pubitemid 27458965)
24. Van den Belt K, Verheyen R, Witters H. 2003. Comparison of vitellogenin responses in zebrafish and rainbow trout following exposure to environmental estrogens. Ecotoxicol Environ Saf 56: 271-281. (Pubitemid 36969950)
25. Harries JE, Runnalls T, Hill E, Harris CA, Maddix S, Sumpter JP, Tyler CR. 2000. Development of a reproductive performance test for endocrine disrupting chemicals using pair-breeding fathead minnows (Pimephales promelas). Environ Sci Technol 34:3003-3011. (Pubitemid 30495660)
26. Christiansen LB, Pedersen KL, Pedersen SN, Korsgaard B, Bjerregaard P. 2000. In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system. Environ Toxicol Chem 19:1867-1874.
27. Kim EJ, Kim JW, Lee SK. 2002. Inhibition of oocyte development in Japanese medaka (Oryzias latipes) exposed to di-2-ethylhexyl phthalate. Environ Int 28:359-365.
28. Gyling M, Leclercq G. 1988. Estrogen and antiestrogcn interaction with estrogen receptor. J Steroid Biochem 29:1-8.
29. Kuiper GGJM, Lemmen JG, Carlsson B, Corton JC, Safe SH, van der Saag PT, van der Burg P, Gustafsson JA. 1998. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology 139:4252-4263. (Pubitemid 28513713)
30. Denny JS, Tapper MA, Schmieder PK, Hornung MW, Jensen KM, Ankley GT, Henry TR. 2005. Comparison of relative binding affinities of endocrine active compounds to fathead minnow and rainbow trout estrogen receptors. Environ Toxicol Chem 24:2948-2953.