Statistical and economical efficiency in assessment of liver regeneration using defined sample size and selection in combination with a fully automated image analysis system

Journal of Histochemistry and Cytochemistry

Volumn 57, Issue 11, 2009, Pages 1075-1085

  Deng, Meihong ^a   Kleinert, Robert ^a   Huang, Hai ^a   He, Qing ^a   Madrahimova, Fotima ^a   Dirsch, Olaf ^b   Dahmen, Uta ^a  

^a UNIVERSITY HOSPITAL ESSEN   (Germany)

^b DEUTSCHES HERZZENTRUM BERLIN   (Germany)

Author keywords

BrdU LI; Computer based fully automatic counting; Liver regeneration; Quantitative immunohistochemistry; Regions of interest

Indexed keywords

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOANALYSIS; AUTOANALYZER; CELL PROLIFERATION; COMPUTER ANALYSIS; CONTROLLED STUDY; ECONOMIC EVALUATION; IMAGE ANALYSIS; IMMUNOHISTOCHEMISTRY; LIVER; LIVER CELL; LIVER REGENERATION; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; SAMPLING; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; STATISTICAL ANALYSIS; VALIDATION PROCESS; ANIMAL; AUTOMATION; CYTOLOGY; ECONOMICS; IMAGE PROCESSING; LEWIS RAT; METABOLISM; METHODOLOGY; OBSERVER VARIATION; REPRODUCIBILITY; SAMPLE SIZE; STAINING; TIME;

BROXURIDINE;

ANIMALS; AUTOMATION; BROMODEOXYURIDINE; CELL PROLIFERATION; HEPATOCYTES; IMAGE PROCESSING, COMPUTER-ASSISTED; LIVER REGENERATION; MALE; OBSERVER VARIATION; RATS; RATS, INBRED LEW; REPRODUCIBILITY OF RESULTS; SAMPLE SIZE; STAINING AND LABELING; TIME FACTORS;

EID: 70449729889     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.953869     Document Type: Article

References (19)

1. Bahnemann R, Mellert W (1997) Lobule-dependent zonal measurement (LZM) method for the determination of cell proliferation in the liver. Exp Toxicol Pathol 49:189-196
2. Chen ZY, White CC, He CY, Liu YF, Eaton DL (1995) Zonal differences in DNA synthesis activity and cytochrome P450 gene expression in livers of male F344 rats treated with five nongenotoxic carcinogens. J Environ Pathol Toxicol Oncol 14:83-99
3. deFazio A, Leary JA, Hedley DW, Tattersall MH (1987) Immunohistochemical detection of proliferating cells in vivo. J Histochem Cytochem 35:571-577
4. Dirsch O, Madrahimov N, Chaudri N, Deng M, Madrahimova F, Schenk A, Dahmen U (2008) Recovery of liver perfusion after focal outflow obstruction and liver resection. Transplantation 85:748-756
5. Dolbeare F (1996) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization. Histochem J 28:531-575
6. Fabrikant JI (1968) The kinetics of cellular proliferation in regenerating liver. J Cell Biol 36:551-565
7. Gandillet A, Alexandre E, Holl V, Royer C, Bischoff P, Cinqualbre J, Wolf P, et al. (2003) Hepatocyte ploidy in normal young rat. Comp Biochem Physiol A Mol Integr Physiol 134:665-673
8. Gebhardt R, Jonitza D (1991) Different proliferative responses of periportal and perivenous hepatocytes to EGF. Biochem Biophys Res Commun 181:1201-1207
9. Gundersen HJ, Jensen EB, Kieu K, Nielsen J (1999) The efficiency of systematic sampling in stereology: reconsidered. J Microsc 193:199-211
10. Madrahimov N, Dirsch O, Broelsch C, Dahmen U (2006) Marginal hepatectomy in the rat: from anatomy to surgery. Ann Surg 244:89-98
11. Marcos R, Monteiro RA, Rocha E (2006) Design-based stereological estimation of hepatocyte number, by combining the smooth optical fractionator and immunocytochemistry with anti-carcinoembryonic antigen polyclonal antibodies. Liver Int 26:116-124
12. Mühlfeld C, Nyengaard JR, Mayhew TM (In Press) A review of state-of-the-art stereology for better quantitative 3D morphology in cardiac research. Cardiovasc Pathol. Published online January 12, 2009 (DOI: 10.1016/j.carpath.2008.10.015)
13. Nolte T, Kaufmann W, Schorsch F, Soames T, Weber E (2005) Standardized assessment of cell proliferation: the approach of the RITA-CEPA working group. Exp Toxicol Pathol 57:91-103
14. Ruehl-Fehlert C, Kittel B, Morawietz G, Deslex P, Keenan C, Mahrt CR, Nolte T, et al. (2003) Revised guides for organ sampling and trimming in rats and mice: part 1. Exp Toxicol Pathol 55:91-106
15. Schmitz C, Hof PR (2000) Recommendations for straightforward and rigorous methods of counting neurons based on a computer simulation approach. J Chem Neuroanat 20:93-114
16. Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB (1994) Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem 42:939-944
17. Thung SN, Gerber MA (1991) Liver. In Sternberg SS, ed. Histology for Pathologists. New York, Raven Press, 626-629
18. Urade M, Izumi R, Kitagawa H (1996) Inhibition of 5-lipoxygenase promotes the regeneration of the liver after partial hepatectomy in normal and icteric rats. Hepatology 23:544-548
19. Wagenaar GT, Chamuleau RA, Pool CW, de Haan JG, Maas MA, Korfage HA, Lamers WH (1993) Distribution and activity of glutamine synthase and carbamoylphosphate synthase upon enlargement of the liver lobule by repeated partial hepatectomies. J Hepatol 17:397-407