Fluorescent homogeneous immunosensors for detecting pathogenic bacteria

Analytical Biochemistry

Volumn 396, Issue 2, 2010, Pages 298-303

  Heyduk, Ewa ^a   Heyduk, Tomasz ^a  

^a SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antibody; Bacteria; Fluorescence; FRET; Pathogen; Sensor

Indexed keywords

ANNEALING; ANTIBODIES; BACTERIA; CELL MEMBRANES; CYTOLOGY; ENERGY TRANSFER; EPITOPES; ESCHERICHIA COLI; FLUORESCENCE; FORSTER RESONANCE ENERGY TRANSFER; OLIGONUCLEOTIDES; PATHOGENS; SALMONELLA; SENSORS;

ESCHERICHIA COLI 0157:H7; FLUORESCENCE PROBES; FLUORESCENCE RESONANCE ENERGY TRANSFER; FRET; HOMOGENEOUS DETECTIONS; OLIGONUCLEOTIDE SEQUENCES; PATHOGENIC BACTERIUM; SALMONELLA TYPHIMURIUM;

CHEMICAL DETECTION;

BACTERIUM ANTIBODY; EPITOPE; FLUOROCHROME; MEMBRANE ANTIGEN; OLIGONUCLEOTIDE;

ARTICLE; BACTERIUM IDENTIFICATION; CELL SURFACE; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMMUNOSENSOR; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN PURIFICATION; REACTION TIME; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; TARGET CELL;

ESCHERICHIA COLI O157:H-; SALMONELLA TYPHIMURIUM;

EID: 70449697904     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.09.039     Document Type: Article

References (15)

1. Gracias K.S., and McKillip J.L. A review of conventional detection and enumeration methods for pathogenic bacteria in food. Can. J. Microbiol. 50 (2004) 883-890
2. Lazcka O., Del Campo F.J., and Munoz F.X. Pathogen detection: a perspective of traditional methods and biosensors. Biosens. Bioelectron. 22 (2007) 1205-1217
3. Leoni E., and Legnani P.P. Comparison of selective procedures for isolation and enumeration of Legionella species from hot water systems. J. Appl. Microbiol. 90 (2001) 27-33
4. Bej A.K., Mahbubani M.H., Dicesare J.L., and Atlas R.M. Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Appl. Environ. Microbiol. 57 (1991) 3529-3534
5. Bej A.K., Mahbubani M.H., Miller R., DiCesare J.L., Haff L., and Atlas R.M. Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water. Mol. Cell. Probes 4 (1990) 353-365
6. Mahbubani M.H., Bej A.K., Miller R., Haff L., DiCesare J., and Atlas R.M. Detection of Legionella with polymerase chain reaction and gene probe methods. Mol. Cell. Probes 4 (1990) 175-187
7. Bej A.K., Steffan R.J., DiCesare J., Haff L., and Atlas R.M. Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Appl. Environ. Microbiol. 56 (1990) 307-314
8. Blais B.W., Bosley J., Martinez-Perez A., and Popela M. Polymyxin-based enzyme-linked immunosorbent assay for the detection of Escherichia coli O111 and O26. J. Microbiol. Methods 65 (2006) 468-475
9. Blais B.W., Leggate J., Bosley J., and Martinez-Perez A. Comparison of fluorogenic and chromogenic assay systems in the detection of Escherichia coli O157 by a novel polymyxin-based ELISA. Lett. Appl. Microbiol. 39 (2004) 516-522
10. Blais B.W., Leggate J., Bosley J., and Martinez-Perez A. Detection of Escherichia coli O157 in foods by a novel polymyxin-based enzyme-linked immunosorbent assay. J. Food Protein 68 (2005) 233-238
11. Heyduk E., and Heyduk T. Nucleic acid-based fluorescence sensors for detecting proteins. Anal. Chem. 77 (2005) 1147-1156
12. Heyduk E., Dummit B., Chang Y.H., and Heyduk T. Molecular pincers: antibody-based homogeneous protein sensors. Anal. Chem. 80 (2008) 5152-5159
13. Selvin P.R. Fluorescence resonance energy transfer. Methods Enzymol. 246 (1995) 300-334
14. Heyduk E., and Heyduk T. Thiol-reactive, luminescent Europium chelates: luminescence probes for resonance energy transfer distance measurements in biomolecules. Anal. Biochem. 248 (1997) 216-227
15. Tian L., and Heyduk T. Bivalent ligands with long nanometer-scale flexible linkers. Biochemistry 48 (2009) 264-275