Mutational analysis of the GTP-binding motif of FlhF which regulates the number and placement of the polar flagellum in vibrio alginolyticus

Journal of Biochemistry

Volumn 146, Issue 5, 2009, Pages 643-650

  Kusumoto, Akiko ^a,b   Nishioka, Noriko ^a   Kojima, Seiji ^a   Homma, Michio ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

Author keywords

Bacterial flagella; GTP binding motif; Polar flagellum

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ALANINE; FLHF PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL MUTATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; VIBRIO ALGINOLYTICUS; WILD TYPE;

ALANINE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL POLARITY; DNA MUTATIONAL ANALYSIS; FLAGELLA; GREEN FLUORESCENT PROTEINS; GUANOSINE TRIPHOSPHATE; IMMUNOPRECIPITATION; MOLECULAR SEQUENCE DATA; MONOMERIC GTP-BINDING PROTEINS; MOVEMENT; MUTANT PROTEINS; MUTATION; PLASMIDS; PROTEIN BINDING; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; VIBRIO ALGINOLYTICUS;

BACTERIA (MICROORGANISMS); VIBRIO ALGINOLYTICUS;

EID: 70449657452     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvp109     Document Type: Article

References (21)

1. McCarter, L.L. (2001) Polar flagellar motility of the Vibrionaceae. Microbiol. Mol. Biol. Rev. 65, 445-462
2. Correa, N.E., Peng, F., and Klose, K.E. (2005) Roles of the regulatory proteins FlhF and FlhG in the Vibrio cholerae flagellar transcription hierarchy. J. Bacteriol. 187, 6324-6332
3. Kusumoto, A., Kamisaka, K., Yakushi, T., Terashima, H., Shinohara, A., and Homma, M. (2006) Regulation of polar flagellar number by the flhF and flhG genes in Vibrio alginolyticus. J. Biochem. 139, 113-121
4. Kusumoto, A., Shinohara, A., Terashima, H., Kojima, S., Yakushi, T., and Homma, M. (2008) Collaboration of FlhF and FlhG to regulate polar-flagella number and localization in Vibrio alginolyticus. Microbiology 154, 1390-1399
5. Murray, T.S. and Kazmierczak, B.I. (2006) FlhF is required for swimming and swarming in Pseudomonas aeruginosa. J. Bacteriol. 188, 6995-7004
6. Pandza, S., Baetens, M., Park, C.H., Au, T., Keyhan, M., and Matin, A. (2000) The G-protein FlhF has a role in polar flagellar placement and general stress response induction in Pseudomonas putida. Mol. Microbiol. 36, 414-423
7. Salvetti, S., Ghelardi, E., Celandroni, F., Ceragioli, M., Giannessi, F., and Senesi, S. (2007) FlhF, a signal recognition particle-like GTPase, is involved in the regulation of flagellar arrangement, motility behaviour and protein secretion in Bacillus cereus. Microbiology 153, 2541-2552
8. Karnoub, A.E. and Weinberg, R.A. (2008) Ras oncogenes: split personalities. Nat. Rev. Mol. Cell Biol. 9, 517-531
9. Dasgupta, N. and Ramphal, R. (2001) Interaction of the antiactivator FleN with the transcriptional activator FleQ regulates flagellar number in Pseudomonas aeruginosa. J. Bacteriol. 183, 6636-6644
10. Dasgupta, N., Ferrell, E.P., Kanack, K.J., West, S.E., and Ramphal, R. (2002) fleQ, the gene encoding the major flagellar regulator of Pseudomonas aeruginosa, is sigma70 dependent and is downregulated by Vfr, a homolog of Escherichia coli cyclic AMP receptor protein. J. Bacteriol. 184, 5240-5250
11. Prouty, M. G., Correa, N.E., and Klose, K.E. (2001) The novel -54- and -28-dependent flagellar gene transcription hierarchy of Vibrio cholerae. Mol. Microbiol. 39, 1595-1609
12. Doudna, J.A. and Batey, R.T. (2004) Structural insights into the signal recognition particle. Ann. Rev. Biochem. 73, 539-557
13. Shan, S.O. and Walter, P. (2005) Co-translational protein targeting by the signal recognition particle. FEBS Lett. 579, 921-926 (Pubitemid 40361866)
14. Kawagishi, I., Okunishi, I., Homma, M., and Imae, Y. (1994) Removal of the periplasmic DNAse before electroporation enhances efficiency of transformation in the marine bacterium Vibrio alginolyticus. Microbiology 140, 2355-2361
15. Kjeldgaard, M., Nyborg, J., and Clark, B.F. (1996) The GTP binding motif: variations on a theme. FASEB J. 10, 1347-1368
16. Dasgupta, N., Arora, S.K., and Ramphal, R. (2000) fleN, a gene that regulates flagellar number in Pseudomonas aeruginosa. J. Bacteriol. 182, 357-364
17. Noble, C.G. and Song, H. (2008) Structural studies of elongation and release factors. Cell Mol. Life Sci. 65, 1335-1346
18. Bange, G., Petzold, G., Wild, K., Parlitz, R.O., and Sinning, I. (2007) The crystal structure of the third signalrecognition particle GTPase FlhF reveals a homodimer with bound GTP. Proc. Natl Acad. Sci. USA 104, 13621-13625
19. Freymann, D.M., Keenan, R.J., Stroud, R.M., and Walter, P. (1997) Structure of the conserved GTPase domain of the signal recognition particle. Nature 385, 361-364
20. Okunishi, I., Kawagishi, I., and Homma, M. (1996) Cloning and characterization of motY, a gene coding for a component of the sodium-driven flagellar motor in Vibrio alginolyticus. J. Bacteriol. 178, 2409-2415
21. Guzman, L.M., Belin, D., Carson, M.J., and Beckwith, J. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177, 4121-4130