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Volumn 3, Issue 3, 2009, Pages 291-301

Microfluidic bioreactors for highly efficient proteolysis

Author keywords

Microfluidic bioreactor; Protease; Proteolysis; Proteomics; Trypsin

Indexed keywords

DIMETICONE; POLY(METHYL METHACRYLATE); TEREPHTHALIC ACID; TRYPSIN;

EID: 70449652454     PISSN: 18723136     EISSN: None     Source Type: Journal    
DOI: 10.2174/187231309789054814     Document Type: Review
Times cited : (11)

References (55)
  • 1
    • 11944262834 scopus 로고
    • Capillary electrophoresis and sample injection systems integrated on a planar glass chip
    • Harrison DJ, Manz A, Fan Z, Ludi H, Widmer HM. Capillary electrophoresis and sample injection systems integrated on a planar glass chip. Anal Chem 1992; 64: 1926-1932
    • (1992) Anal Chem , vol.64 , pp. 1926-1932
    • Harrison, D.J.1    Manz, A.2    Fan, Z.3    Ludi, H.4    Widmer, H.M.5
  • 2
    • 0342507903 scopus 로고
    • Micromachining a miniaturized capillary electrophoresis-based chemical analysis system on a chip
    • Harrison DJ, Flury K, Seiler K, Fan Z, Effenhauser CS, Manz A. Micromachining a miniaturized capillary electrophoresis -based chemical analysis system on a chip. Science 1993; 261: 895-897 (Pubitemid 23982813)
    • (1993) Science , vol.261 , Issue.5123 , pp. 895-897
    • Harrison, D.J.1    Fluri, K.2    Seiler, K.3    Fan, Z.4    Effenhauser, C.S.5    Manz, A.6
  • 3
    • 0037096666 scopus 로고    scopus 로고
    • Micro total analysis systems. 1. Introduction, theory, and technology
    • DOI 10.1021/ac0202435
    • Reyes DR, Iossifidis D, Auroux PA, Manz A. Micro total analysis systems, 1 introduction, theory, and technology. Anal Chem 2002; 74: 2623-2636 (Pubitemid 34651165)
    • (2002) Analytical Chemistry , vol.74 , Issue.12 , pp. 2623-2636
    • Reyes, D.R.1    Iossifidis, D.2    Auroux, P.-A.3    Manz, A.4
  • 4
    • 33846404902 scopus 로고    scopus 로고
    • Carbon nanotube/PMMA composite electrode fabricated by in situ polymerization for microchip capillary electrophoresis
    • Yao X, Wu HX, Wang J, Qu S, Chen G. Carbon nanotube/PMMA composite electrode fabricated by in situ polymerization for microchip capillary electrophoresis. Chem Eur J 2007; 13: 846-853
    • (2007) Chem Eur J , vol.13 , pp. 846-853
    • Yao, X.1    Wu, H.X.2    Wang, J.3    Qu, S.4    Chen, G.5
  • 5
    • 33745254515 scopus 로고    scopus 로고
    • Micro total analysis systems. Latest advancements and trends. Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: Simultaneous/ electrochemical measurement of ethanol and glucose
    • Dittrich PS, Tachikawa K, Manz A. Micro total analysis systems. Latest advancements and trends. Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: simultaneous/ electrochemical measurement of ethanol and glucose. Anal Chem 2006; 78: 3887-3908
    • (2006) Anal Chem , vol.78 , pp. 3887-3908
    • Dittrich, P.S.1    Tachikawa, K.2    Manz, A.3
  • 7
    • 0034212524 scopus 로고    scopus 로고
    • Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: Simultaneous electrochemical measurement of ethanol and glucose
    • Wang J, Chatrathi MP, Tian B, Polsky R. Microseparation chips for performing multienzymatic dehydrogenase/oxidase assays: simultaneous electrochemical measurement of ethanol and glucose. Anal Chem 2000; 72: 2514-2518
    • (2000) Anal Chem , vol.72 , pp. 2514-2518
    • Wang, J.1    Chatrathi, M.P.2    Tian, B.3    Polsky, R.4
  • 8
    • 0032738605 scopus 로고    scopus 로고
    • Microfluidic assays of acetylcholinesterase inhibitors
    • Hadd AG, Jacobson SC, Ramsey JM. Microfluidic assays of acetylcholinesterase inhibitors. Anal Chem 1999; 71: 5206-12.
    • (1999) Anal Chem , vol.71 , pp. 5206-5212
    • Hadd, A.G.1    Jacobson, S.C.2    Ramsey, J.M.3
  • 9
    • 0038713935 scopus 로고    scopus 로고
    • On-chip integration of enzyme and immunoassays: Simultaneous measurements of insulin and glucose
    • Wang J, Ibanez A, Chatrathi MP. On-chip integration of enzyme and immunoassays: simultaneous measurements of insulin and glucose. J Am Chem Soc 2003; 125: 8444-8445
    • (2003) J Am Chem Soc , vol.125 , pp. 8444-8445
    • Wang, J.1    Ibanez, A.2    Chatrathi, M.P.3
  • 10
    • 0034654422 scopus 로고    scopus 로고
    • Integration of an immunosorbent assay system: Analysis of secretory human. Immunoglobulin a on polystyrene beads in a microchip
    • Sato K, Tokeshi M, Odake T, et al. Integration of an immunosorbent assay system: analysis of secretory human. Immunoglobulin a on polystyrene beads in a microchip. Anal Chem 2000; 72: 1144-1147
    • (2000) Anal Chem , vol.72 , pp. 1144-1147
    • Sato, K.1    Tokeshi, M.2    Odake, T.3
  • 11
    • 0028788811 scopus 로고
    • From genome to proteome: Looking at a cell's proteins
    • Kahn P. From genome to proteome: Looking at a cell's proteins. Science 1995; 270: 369-370
    • (1995) Science , vol.270 , pp. 369-370
    • Kahn, P.1
  • 12
    • 0031848226 scopus 로고    scopus 로고
    • Proteome and proteomics: New technologies, new concepts, and new words
    • Anderson NL, Anderson NG. Proteome and proteomics: New technologies, new concepts, and new words. Electrophoresis 1998; 19: 1853-1861
    • (1998) Electrophoresis , vol.19 , pp. 1853-1861
    • Anderson, N.L.1    Anderson, N.G.2
  • 13
    • 0034212611 scopus 로고    scopus 로고
    • Thermal denaturation: A useful technique in peptide mass mapping
    • Park ZY, Russell DH. Thermal denaturation: A useful technique in peptide mass mapping. Anal Chem 2000; 72: 2667-2670
    • (2000) Anal Chem , vol.72 , pp. 2667-2670
    • Park, Z.Y.1    Russell, D.H.2
  • 14
    • 33747202736 scopus 로고    scopus 로고
    • Detection and identification of sub-nanogram levels of protein in a nanoLC-trypsin-MS system
    • Slysz GW, Lewis DF, Schriemer DC. Detection and identification of sub-nanogram levels of protein in a nanoLC-trypsin-MS system. J Proteome Res 2006; 5: 1959-1966
    • (2006) J Proteome Res , vol.5 , pp. 1959-1966
    • Slysz, G.W.1    Lewis, D.F.2    Schriemer, D.C.3
  • 15
    • 0037315064 scopus 로고    scopus 로고
    • Creation of an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip
    • DOI 10.1021/ac026240+
    • Sakai-Kato K, Kato M, Toyooka T. Creation of an on-chip enzyme reactor by encapsulating trypsin in sol-gel on a plastic microchip. Anal Chem 2003; 75: 388-393 (Pubitemid 36176707)
    • (2003) Analytical Chemistry , vol.75 , Issue.3 , pp. 388-393
    • Sakai-Kato, K.1    Kato, M.2    Toyo'Oka, T.3
  • 16
    • 20244367101 scopus 로고    scopus 로고
    • Titania and alumina sol-gel-derived microfluidics enzymatic-reactors for peptide mapping: Design, characterization, and performance
    • Wu HL, Tian YP, Liu BH, et al. Titania and alumina sol-gel-derived microfluidics enzymatic-reactors for peptide mapping: design, characterization, and performance. J Proteome Res 2004; 3: 1201-1209
    • (2004) J Proteome Res , vol.3 , pp. 1201-1209
    • Wu, H.L.1    Tian, Y.P.2    Liu, B.H.3
  • 17
    • 7644236036 scopus 로고    scopus 로고
    • Stable microstructured network for protein patterning on a plastic microfluidic channel: Strategy and characterization of on-chip enzyme microreactors
    • DOI 10.1021/ac049466g
    • Qu HY, Wang HT, Huang Y, et al. Stable microstructured network for protein patterning on a plastic microfluidic channel: Strategy and characterization of on-chip enzyme microreactors. Anal Chem 2004; 76: 6426-6433 (Pubitemid 39458478)
    • (2004) Analytical Chemistry , vol.76 , Issue.21 , pp. 6426-6433
    • Qu, H.1    Wang, H.2    Huang, Y.3    Zhong, W.4    Lu, H.5    Kong, J.6    Yang, P.7    Liu, B.8
  • 18
    • 33645420692 scopus 로고    scopus 로고
    • Zeolite nanoparticle modified microchip reactor for efficient protein digestion
    • Huang Y, Shan W, Liu BH, et al. Zeolite nanoparticle modified microchip reactor for efficient protein digestion. Lab Chip 2006; 6: 534-539
    • (2006) Lab Chip , vol.6 , pp. 534-539
    • Huang, Y.1    Shan, W.2    Liu, B.H.3
  • 19
    • 33748562851 scopus 로고    scopus 로고
    • Zeolite nanoparticle modified microchip reactor for efficient protein digestion
    • Zhang YH, Liu Y, Kong JL, Yang PY, Tang Y, Liu BH. Zeolite nanoparticle modified microchip reactor for efficient protein digestion. Small 2006; 2: 1170-1173
    • (2006) Small , vol.2 , pp. 1170-1173
    • Zhang, Y.H.1    Liu, Y.2    Kong, J.L.3    Yang, P.Y.4    Tang, Y.5    Liu, B.H.6
  • 20
    • 0037102287 scopus 로고    scopus 로고
    • Enzymatic microreactor-on-a-chip: Protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices
    • DOI 10.1021/ac020180q
    • Peterson DS, Rohr T, Svec F, Frechet JMJ. Enzymatic microreactor-on-a- chip: Protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices. Anal Chem 2002; 74: 4081-4088 (Pubitemid 34912908)
    • (2002) Analytical Chemistry , vol.74 , Issue.16 , pp. 4081-4088
    • Peterson, D.S.1    Rohr, T.2    Svec, F.3    Frechet, J.M.J.4
  • 21
    • 0347134475 scopus 로고    scopus 로고
    • Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry
    • DOI 10.1002/elps.200305621
    • Guo Z, Xu S, Lei Z, Zou H, Guo B. Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry. Electrophoresis 2003; 24: 3633-3639 (Pubitemid 38089165)
    • (2003) Electrophoresis , vol.24 , Issue.21 , pp. 3633-3639
    • Guo, Z.1    Xu, S.2    Lei, Z.3    Zou, H.4    Guo, B.5
  • 23
    • 32444450698 scopus 로고    scopus 로고
    • Multilayer-assembled microchip for enzyme immobilization as reactor toward low-level protein identification
    • DOI 10.1021/ac051463w
    • Liu Y, Lu HJ, Zhong W, et al. Multilayer-assembled microchip for enzyme immobilization as reactor toward low-level protein identification. Anal Chem 2006; 78: 801-808 (Pubitemid 43228588)
    • (2006) Analytical Chemistry , vol.78 , Issue.3 , pp. 801-808
    • Liu, Y.1    Lu, H.2    Zhong, W.3    Song, P.4    Kong, J.5    Yang, P.6    Girault, H.H.7    Liu, B.8
  • 24
    • 0000974307 scopus 로고
    • On-line microrectors/capillary electrophoresis/mass. spectrometry for the analysis of proteins and peptides
    • Licklider L, Kuhr WG, Lacoy MP, Keough T, Purdon MP. On-line microrectors/capillary electrophoresis/mass. spectrometry for the analysis of proteins and peptides. Anal Chem 1995; 67: 4170-4177
    • (1995) Anal Chem , vol.67 , pp. 4170-4177
    • Licklider, L.1    Kuhr, W.G.2    Lacoy, M.P.3    Keough, T.4    Purdon, M.P.5
  • 25
    • 0027120058 scopus 로고
    • Peptide mapping of complex proteins at the low-picomole level with capillary electrophoretic separations
    • Cobbt KA, Novotny MV. Peptide mapping of complex proteins at the low-picomole level with capillary electrophoretic separations. Anal Chem 1992; 64: 879-886
    • (1992) Anal Chem , vol.64 , pp. 879-886
    • Cobbt, K.A.1    Novotny, M.V.2
  • 26
    • 36949024716 scopus 로고    scopus 로고
    • Chymotrypsin immobilization on epoxy monolithic silica columns: Development and characterization of a bioreactor for protein digestion
    • DOI 10.1002/jssc.200700337
    • Temporini C, Calleri E, Campese D, Cabrera K, Felix G, Massolini G. Chymotrypsin immobilization on epoxy monolithic silica columns: development and characterization of a bioreactor for protein digestion. J Sep Sci 2007; 30: 3069-3076 (Pubitemid 350238342)
    • (2007) Journal of Separation Science , vol.30 , Issue.17 , pp. 3069-3076
    • Temporini, C.1    Calleri, E.2    Campese, D.3    Cabrera, K.4    Felix, G.5    Massolini, G.6
  • 27
    • 34948845083 scopus 로고    scopus 로고
    • Immobilization of trypsin on superparamagnetic nanoparticles for rapid and effective proteolysis
    • DOI 10.1021/pr070132s
    • Li Y, Xu XQ, Deng CH, Yang PY, Zhang XM. Immobilization of trypsin on superparamagnetic nanoparticles for rapid and effective proteolysis. J Proteome Res 2007; 6: 3849-3855 (Pubitemid 47522774)
    • (2007) Journal of Proteome Research , vol.6 , Issue.9 , pp. 3849-3855
    • Li, Y.1    Xu, X.2    Deng, C.3    Yang, P.4    Zhang, X.5
  • 28
    • 36849066250 scopus 로고    scopus 로고
    • Development of microwave-assisted protein digestion based on trypsin-immobilized magnetic microspheres for highly efficient proteolysis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
    • Lin S, Lin ZX, Yao GP, Deng CH, Yang PY, Zhang XM. Development of microwave-assisted protein digestion based on trypsin-immobilized magnetic microspheres for highly efficient proteolysis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis. Rapid Commun Mass Spectrom 2007; 21: 3910-3918
    • (2007) Rapid Commun Mass Spectrom , vol.21 , pp. 3910-3918
    • Lin, S.1    Lin, Z.X.2    Yao, G.P.3    Deng, C.H.4    Yang, P.Y.5    Zhang, X.M.6
  • 29
    • 33947427539 scopus 로고    scopus 로고
    • Acceleration of microwave-assisted enzymatic digestion reactions by magnetite beads
    • Chen WY, Chen YC. Acceleration of microwave-assisted enzymatic digestion reactions by magnetite beads. Anal Chem 2007; 79: 2394-2401
    • (2007) Anal Chem , vol.79 , pp. 2394-2401
    • Chen, W.Y.1    Chen, Y.C.2
  • 30
    • 10644233246 scopus 로고    scopus 로고
    • Microfluidic enzymatic-reactors for peptide mapping: Strategy, characterization, and performance
    • Wu HL, Zhai JJ, Tian YP, et al. Microfluidic enzymatic-reactors for peptide mapping: strategy, characterization, and performance. Lab Chip 2004; 4: 588-597
    • (2004) Lab Chip , vol.4 , pp. 588-597
    • Wu, H.L.1    Zhai, J.J.2    Tian, Y.P.3
  • 31
    • 45849083145 scopus 로고    scopus 로고
    • Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS
    • Lee J, Musyimi HK, Soper SA, Murray KK. Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS. J Am Soc Mass Spectrom 2008; 19: 964-972
    • (2008) J Am Soc Mass Spectrom , vol.19 , pp. 964-972
    • Lee, J.1    Musyimi, H.K.2    Soper, S.A.3    Murray, K.K.4
  • 32
    • 34548410545 scopus 로고    scopus 로고
    • Gold nanoparticle assembly microfluidic reactor for efficient on-line proteolysis
    • Liu Y, Xue Y, Ji J, et al. Gold nanoparticle assembly microfluidic reactor for efficient on-line proteolysis. Mol Cell Proteomics 2007; 6: 1428-1436
    • (2007) Mol Cell Proteomics , vol.6 , pp. 1428-1436
    • Liu, Y.1    Xue, Y.2    Ji, J.3
  • 33
    • 41849085229 scopus 로고    scopus 로고
    • Enhanced protein digestion through the confinement of nanozeolite-assembled microchip reactors
    • Ji J, Zhang YH, Zhou XQ, Kong JL, Tang Y, Liu BH. Enhanced protein digestion through the confinement of nanozeolite-assembled microchip reactors. Anal Chem 2008; 80: 2457-2463
    • (2008) Anal Chem , vol.80 , pp. 2457-2463
    • Ji, J.1    Zhang, Y.H.2    Zhou, X.Q.3    Kong, J.L.4    Tang, Y.5    Liu, B.H.6
  • 34
    • 0035356224 scopus 로고    scopus 로고
    • Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification
    • Gao J, Xu JD, Locascio LE, Lee CS. Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification. Anal Chem 2001; 73: 2648-2655
    • (2001) Anal Chem , vol.73 , pp. 2648-2655
    • Gao, J.1    Xu, J.D.2    Locascio, L.E.3    Lee, C.S.4
  • 35
    • 0035958529 scopus 로고    scopus 로고
    • On-line coupling of micro-enzyme reactor with micro-membrane chromatography for protein digestion, peptide separation, and protein identification using electrospray ionization mass spectrometry
    • Jiang Y, Lee CS. On-line coupling of micro-enzyme reactor with micro-membrane chromatography for protein digestion, peptide separation, and protein identification using electrospray ionization mass spectrometry. J Chromatogr A 2001; 924: 315-322
    • (2001) J Chromatogr A , vol.924 , pp. 315-322
    • Jiang, Y.1    Lee, C.S.2
  • 36
    • 34248675246 scopus 로고    scopus 로고
    • Enhancement of proteolysis through the silica-gel-derived microfluidic reactor
    • DOI 10.1002/pmic.200600896
    • Liu Y, Qu HYn, Xue Yn, Wu ZL, Yang PY, Liu BH. Enhancement of proteolysis through the silica-gel-derived microfluidic reactor. Proteomics 2007; 7: 1373-1378 (Pubitemid 46775872)
    • (2007) Proteomics , vol.7 , Issue.9 , pp. 1373-1378
    • Liu, Y.1    Qu, H.2    Xue, Y.3    Wu, Z.4    Yang, P.5    Liu, B.6
  • 37
    • 38849095967 scopus 로고    scopus 로고
    • Controlled proteolysis of normal and pathological prion protein in a microfluidic chip
    • Le Nel A, Minc N, Smadja C1, et al. Controlled proteolysis of normal and pathological prion protein in a microfluidic chip. Lab Chip 2008; 8: 294-301.
    • (2008) Lab Chip , vol.8 , pp. 294-301
    • Le Nel, A.1    Minc, N.2    Smadja, C.3
  • 38
    • 24744450131 scopus 로고    scopus 로고
    • Use of self assembled magnetic beads for on-chip protein digestion
    • Slovakova M, Minc N, Bilkova Z, et al. Use of self assembled magnetic beads for on-chip protein digestion. Lab Chip 2005; 5: 935-942.
    • (2005) Lab Chip , vol.5 , pp. 935-942
    • Slovakova, M.1    Minc, N.2    Bilkova, Z.3
  • 39
    • 36549071056 scopus 로고    scopus 로고
    • On-chip enzymatic microreactor using trypsin-immobilized superparamagnetic nanoparticles for highly efficient proteolysis
    • Liu JY, Lin S, Qi DW, Deng CH, Yang PY, Zhang XM. On-chip enzymatic microreactor using trypsin-immobilized superparamagnetic nanoparticles for highly efficient proteolysis. J Chromatogr A 2007; 1176: 169-177
    • (2007) J Chromatogr A , vol.1176 , pp. 169-177
    • Liu, J.Y.1    Lin, S.2    Qi, D.W.3    Deng, C.H.4    Yang, P.Y.5    Zhang, X.M.6
  • 40
    • 34547179265 scopus 로고    scopus 로고
    • Efficient on-chip proteolysis system based on functionalized magnetic silica microspheres
    • DOI 10.1002/pmic.200700112
    • Li Y, Yan B, Deng CH, et al. Efficient on-chip proteolysis system based on functionalized magnetic silica microspheres. Proteomics 2007; 7: 2330-2339 (Pubitemid 47121524)
    • (2007) Proteomics , vol.7 , Issue.14 , pp. 2330-2339
    • Li, Y.1    Yan, B.2    Deng, C.3    Yu, W.4    Xu, X.5    Yang, P.6    Zhang, X.7
  • 41
    • 34250860141 scopus 로고    scopus 로고
    • Microchip reactor packed with metalion chelated magnetic silica microspheres for highly efficient proteolysis
    • Li Y, Xu XQ, Yan B, et al. Microchip reactor packed with metalion chelated magnetic silica microspheres for highly efficient proteolysis. J Proteome Res 2007; 6: 2367-2375
    • (2007) J Proteome Res , vol.6 , pp. 2367-2375
    • Li, Y.1    Xu, X.Q.2    Yan, B.3
  • 42
    • 33646584559 scopus 로고    scopus 로고
    • Functionalized magnetic micro- And nanoparticles: Optimization and application to μ-chip tryptic digestion
    • Bilkoval Z, Slovakova M, Minc N, et al. Functionalized magnetic micro- and nanoparticles: optimization and application to μ-chip tryptic digestion. Electrophoresis 2006; 27: 1811-1824
    • (2006) Electrophoresis , vol.27 , pp. 1811-1824
    • Bilkoval, Z.1    Slovakova, M.2    Minc, N.3
  • 43
    • 0346371084 scopus 로고    scopus 로고
    • A microchip-based proteolytic digestion system driven by electroosmotic pumping
    • Jin LJ, Ferrance J, Sanders JC, Landers JP. A microchip-based proteolytic digestion system driven by electroosmotic pumping. Lab Chip 2003; 3: 1-18.
    • (2003) Lab Chip , vol.3 , pp. 1-18
    • Jin, L.J.1    Ferrance, J.2    Sanders, J.C.3    Landers, J.P.4
  • 44
    • 0033868723 scopus 로고    scopus 로고
    • Integration of immobilized trypsin bead beds for protein digestion within a microfluidic chip incorporating capillary electrophoresis separations and an electrospray mass spectrometry interface
    • Wang C, Oleschuk R, Ouchen F, Li JJ, Thibault P, Harrison D.J. Integration of immobilized trypsin bead beds for protein digestion within a microfluidic chip incorporating capillary electrophoresis separations and an electrospray mass spectrometry interface. Rapid Commun Mass Spectrom 2000; 14: 1377-1383
    • (2000) Rapid Commun Mass Spectrom , vol.14 , pp. 1377-1383
    • Wang, C.1    Oleschuk, R.2    Ouchen, F.3    Li, J.J.4    Thibault, P.5    Harrison, D.J.6
  • 45
    • 35348987022 scopus 로고    scopus 로고
    • Fiber-packed channel bioreactor for microfluidic protein digestion
    • Fan HZ, Chen G. Fiber-packed channel bioreactor for microfluidic protein digestion. Proteomics 2007; 7: 3445-3449
    • (2007) Proteomics , vol.7 , pp. 3445-3449
    • Fan, H.Z.1    Chen, G.2
  • 46
    • 58149513103 scopus 로고    scopus 로고
    • Immobilization of trypsin on silica-coated fiberglass core in microchip for highly efficient proteolysis
    • Liu T, Wang S, Chen G. Immobilization of trypsin on silica-coated fiberglass core in microchip for highly efficient proteolysis. Talanta 2009; 77: 1767-1773
    • (2009) Talanta , vol.77 , pp. 1767-1773
    • Liu, T.1    Wang, S.2    Chen, G.3
  • 47
    • 33751534187 scopus 로고    scopus 로고
    • Integration of an on-line protein digestion microreactor to a nanoelectrospray emitter for peptide mapping
    • Zhao C, Jiang HH, Smith DR, Bruckenstein S, Wood TD. Integration of an on-line protein digestion microreactor to a nanoelectrospray emitter for peptide mapping. Anal Biochem 2006; 359: 167-175
    • (2006) Anal Biochem , vol.359 , pp. 167-175
    • Zhao, C.1    Jiang, H.H.2    Smith, D.R.3    Bruckenstein, S.4    Wood, T.D.5
  • 48
    • 34447269003 scopus 로고    scopus 로고
    • Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
    • DOI 10.1016/j.chroma.2007.02.095, PII S0021967307004256
    • Krenkova J, Kleparnik K, Foret F. Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries. J Chromatogr A 2007; 1159: 110-118 (Pubitemid 47048243)
    • (2007) Journal of Chromatography a , vol.1159 , Issue.1-2 , pp. 110-118
    • Krenkova, J.1    Kleparnik, K.2    Foret, F.3
  • 49
    • 42649142273 scopus 로고    scopus 로고
    • Trypsin entrapped in poly(diallyldimethylammonium chloride) silica sol-gel microreactor coupled to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    • DOI 10.1002/rcm.3478
    • Xu XJ, Wang XY, Liu Y, Liu BH, Wu HL, Yang PY. Trypsin entrapped in poly(diallyldimethylammonium chloride) silica solgel microreactor coupled to matrix-assisted laser desorp tion/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2008; 22: 1257-1264 (Pubitemid 351600776)
    • (2008) Rapid Communications in Mass Spectrometry , vol.22 , Issue.8 , pp. 1257-1264
    • Xu, X.1    Wang, X.2    Liu, Y.3    Liu, B.4    Wu, H.5    Yang, P.6
  • 50
    • 0036646541 scopus 로고    scopus 로고
    • On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis
    • DOI 10.1021/ac0200421
    • Sakai-Kato K, Kato M, Toyooka T. On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis. Anal Chem 2002; 74: 2943-2949 (Pubitemid 34755206)
    • (2002) Analytical Chemistry , vol.74 , Issue.13 , pp. 2943-2949
    • Sakai-Kato, K.1    Kato, M.2    Toyo'Oka, T.3
  • 51
    • 0036874368 scopus 로고    scopus 로고
    • High-throughput peptide mass mapping using a microdevice containing trypsin immobilized on a porous polymer monolith coupled to MALDI TOF and ESI TOF mass spectrometers
    • DOI 10.1021/pr0255452
    • Peterson DS, Rohr T, Svec F, Frechet JMJ. High-throughput peptide mass mapping using a microdevice containing trypsin immobilized on a porous polymer monolith coupled to MALDI TOF and ESI TOF mass spectrometers. J Proteome Res 2002; 1: 563-568 (Pubitemid 36395856)
    • (2002) Journal of Proteome Research , vol.1 , Issue.6 , pp. 563-568
    • Peterson, D.S.1    Rohr, T.2    Svec, F.3    Frechet, J.M.J.4
  • 52
    • 42349103426 scopus 로고    scopus 로고
    • Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity
    • DOI 10.1021/ac702343a
    • Ma JF, Liang Z, Qiao XQ, et al. Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity. Anal Chem 2008; 80: 2949-2956 (Pubitemid 351556460)
    • (2008) Analytical Chemistry , vol.80 , Issue.8 , pp. 2949-2956
    • Ma, J.1    Liang, Z.2    Qiao, X.3    Deng, Q.4    Tao, D.5    Zhang, L.6    Zhang, Y.7
  • 53
    • 1842476304 scopus 로고    scopus 로고
    • Integration of On-Line Protein Digestion, Peptide Separation, and Protein Identification Using Pepsin-Coated Photopolymerized Sol-Gel Columns and Capillary Electrophoresis/Mass Spectrometry
    • DOI 10.1021/ac035107u
    • Kato M, Sakai-Kato K, Jin HM, et al. Integration of on-line protein digestion, peptide separation, and protein identification using pepsin- coated photopolymerized sol-gel columns and capillary electrophoresis/ mass spectrometry. Anal Chem 2004; 76: 1896-1902 (Pubitemid 38451505)
    • (2004) Analytical Chemistry , vol.76 , Issue.7 , pp. 1896-1902
    • Kato, M.1    Sakai-Kato, K.2    Jin, H.3    Kubota, K.4    Miyano, H.5    Toyo'Oka, T.6    Dulay, M.T.7    Zare, R.N.8
  • 54
    • 34547154209 scopus 로고    scopus 로고
    • On-column tryptic mapping of proteins using metal-ion-chelated magnetic silica microspheres by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    • DOI 10.1002/rcm.3073
    • Li Y, Yan B, Xu XQ, et al. On-column tryptic mapping of proteins using metal-ion-chelated magnetic silica microspheres by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2007; 21: 2263-2268 (Pubitemid 47121157)
    • (2007) Rapid Communications in Mass Spectrometry , vol.21 , Issue.14 , pp. 2263-2268
    • Li, Y.1    Yan, B.2    Xu, X.3    Deng, C.4    Yang, P.5    Shen, X.6    Zhang, X.7
  • 55
    • 43549107264 scopus 로고    scopus 로고
    • Trypsin-immobilized fiber core in syringe needle for highly efficient proteolysis
    • DOI 10.1002/pmic.200701042
    • Wang S, Zhi Chen, Yang PY, Chen G. Trypsin-immobilized fiber core in syringe needle for highly efficient proteolysis. Proteomics 2008; 8: 1785-1788 (Pubitemid 351678278)
    • (2008) Proteomics , vol.8 , Issue.9 , pp. 1785-1788
    • Wang, S.1    Chen, Z.2    Yang, P.3    Chen, G.4


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