Modified electrophoretic and digestion conditions allow a simplified mass spectrometric evaluation of disulfide bonds

Journal of Mass Spectrometry

Volumn 44, Issue 11, 2009, Pages 1571-1578

  Pompach, Petr ^a,b   Man, Petr ^a,b   Kavan, Daniel ^a,b   Hofbauerová, Kateřina ^a,b   Kumar, Vinay ^a,b   Bezouška, Karel ^a,b   Havlíček, Vladimír ^a,c   Novák, Petr ^a,b  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c PALACKÝ UNIVERSITY   (Czech Republic)

Author keywords

Cystamine; Disulfide bond; FTMS; Gel electrophoresis; Liquid chromatography

Indexed keywords

ASPERGILLUS ORYZAE; CYSTAMINE; DISULFIDE BONDING; DISULFIDE BONDS; FTMS; GEL ELECTROPHORESIS; GLYCOSYLATED; HEN EGG; HIGH PH VALUE; HUMAN LEUKOCYTE ANTIGEN; LEUKOCYTE RECEPTORS; MODEL PROTEINS; MONOMERIC FORMS; OXIDATIVE AGENTS; PROTEIN DIGESTION; PROTEIN HOMOLOGY; RECOMBINANT PROTEIN; SAMPLE HANDLING; STRUCTURE REFINEMENTS;

CHROMATOGRAPHIC ANALYSIS; DIFFUSERS (OPTICAL); ELECTROPHORESIS; ENZYME ACTIVITY; GELS; LIQUID CHROMATOGRAPHY; LIQUIDS; ASPERGILLUS; COVALENT BONDS; SULFUR COMPOUNDS;

PROTEIN FOLDING; RECOMBINANT PROTEINS;

BETA N ACETYLHEXOSAMINIDASE; CD161 ANTIGEN; CD6 ANTIGEN; CYSTAMINE; CYSTINE; DISULFIDE; LYSOZYME;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS ORYZAE; DIGESTION; DISULFIDE BOND; EGG; HUMAN; MASS SPECTROMETRY; MOUSE; NONHUMAN; PENICILLIUM; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN PROCESSING; SAMPLING;

AMINO ACID SEQUENCE; ANIMALS; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEINS;

ASPERGILLUS ORYZAE; MURINAE; PENICILLIUM OXALICUM;

EID: 70449644979     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1609     Document Type: Article

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