The scaled volume as an image analysis variable for detecting changes in protein expression levels by silver stain

Electrophoresis

Volumn 22, Issue 9, 2001, Pages 1627-1632

  Dutt, Michael J ^a   Lee, Kelvin H ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

Author keywords

Proteomics; Silver stain; Two dimensional electrophoresis

Indexed keywords

DENSITY (OPTICAL); DENSITY MEASUREMENT (OPTICAL); ELECTROPHORESIS; GELS; MOLECULAR BIOLOGY; PROTEINS;

IMAGE-ANALYSIS; PROTEIN EXPRESSION LEVELS; PROTEIN SPOTS; PROTEOMICS; RELATIVE INTENSITY; SIGNALS OF INTERESTS; SILVER STAIN; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL GELS; VOLUME RATIO;

SILVER;

EID: 70449626814     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/1522-2683(200105)22:9<1627::AID-ELPS1627>3.0.CO;2-R     Document Type: Article

References (11)

1. Patton, W.F., Electrophoresis 2000, 21, 1123-1144.
2. Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., Aebersold, R., Nature Biotech. 1999, 17, 994-999.
3. Poehling, H.M., Neuhoff, V., Electrophoresis 1981, 2, 141-147.
4. Merril, C.R., Goldman, D., Van Keuren, M.L., Electrophoresis 1982, 3, 17-23.
5. Bini, L., Magi, B., Marzocchi, B., Cellesi, C., Berti, B., Raggiaschi, R., Rossolini, A., Pallini, V., Electrophoresis 1996, 17, 612-616.
6. Yan, J.X., Sanchez, J.C., Tonella, L., Williams, K.L., Hochstrasser, D.F., Electrophoresis 1999, 20, 738-742.
7. Giometti, C.S., Gemmell, M.A., Tollaksen, S.L., Taylor, J., Electrophoresis 1991, 12, 536-543.
8. Hatzimanikatis, V., Choe, L.H., Lee, K.H., Biotechnol. Progr. 1999, 15, 312-318.
9. Anderson, N.L., Nance, S.L., Tollaksen, S.L., Giere, F.A., Anderson, N.G., Electrophoresis 1985, 6, 592-599.
10. Rabilloud, T., Electrophoresis 1990, 11, 785-794.
11. Shuler, M.L., Kargi, F., Bioprocess Engineering: Basic Concepts, Prentice Hall, Englewood Cliffs, NJ 1992, pp. 61-78.