Light scattering characterization of single biological cells in a microfluidic cytometer

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7386, Issue , 2009, Pages

  Su, Xuantao ^a   Kirkwood, Sean E ^a   Gul, Hilal ^a   Singh, Kirat ^a   Islam, Md Z ^a   Anna, Janowska Wieczorek ^a   Rozmus, Wojciech ^a   Tsui, Ying Y ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Cytometer; FDTD; Light scattering; Microfluidics; Pattern analysis; Single cells

Indexed keywords

2D SIMULATIONS; BIOLOGICAL CELLS; CHARGE COUPLED DEVICE DETECTORS; CYTOMETER; FDTD; FLUIDIC FLOW; INNER STRUCTURE; LAB-ON-CHIP; LABEL-FREE DETECTION; LASER LIGHTS; MICROFLUIDIC FLOW; MIE THEORY; NON-HOMOGENEOUS; PATTERN ANALYSIS; POLYSTYRENE BEADS; SIMULATION RESULT; SINGLE CELLS; SINGLE-CELL LEVEL; YEAST CELL;

BIOINFORMATICS; CELLS; CYTOLOGY; FINITE DIFFERENCE TIME DOMAIN METHOD; LIGHT; LIGHT SCATTERING; METHOD OF MOMENTS; OPTICAL FIBERS; OPTICAL MATERIALS; OPTOELECTRONIC DEVICES; POLYMER BLENDS; POLYSTYRENES; REFRACTION; SCATTERING; SIMULATORS; YEAST;

MICROFLUIDICS;

EID: 70449596243     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.837064     Document Type: Conference Paper

References (15)

1. Liu, C. G., Capjack, C. and Rozmus, W., "3-D simulation of light scattering from biological cells and cell differentiation", J. Biomed. Opt., 10 (1), 014007 (2005).
2. Drezek, R., Dunn, A. and Richards-Kortum, R., "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements", Appl. Optics, 38 (16), 3651-3661 (1999).
3. Backman, V., Gopal, V., Kalashnikov, M., Badizadegan, K., Gurjar, R., Wax, A., Georgakoudi, I., Mueller, M., Boone, C. W., Dasari, R. R. and Feld, M. S., "Measuring cellular structure at submicrometer scale with light scattering spectroscopy", IEEE J. Sel. Top. Quantum Electron., 7 (6), 887-893 (2001).
4. Wax, A., Yang, C. H., Backman, V., Badizadegan, K., Boone, C. W., Dasari, R. R. and Feld, M. S., "Cellular organization and substructure measured using angle-resolved low-coherence interferometry", Biophys. J., 82 (4), 2256-2264 (2002).
5. Mourant, J. R., Freyer, J. P., Hielscher, A. H., Eick, A. A., Shen, D. and Johnson, T. M., "Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics", Appl. Optics, 37 (16), 3586-3593 (1998).
6. Su, X. T., Capjack, C., Rozmus, W. and Backhouse, C., "2D light scattering patterns of mitochondria in single cells", Opt. Express, 15 (17), 10562-10575 (2007).
7. Backman, V., Gurjar, R., Badizadegan, K., Itzkan, L., Dasari, R. R., Perelman, L. T. and Feld, M. S., "Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ", IEEE J. Sel. Top. Quantum Electron., 5 (4), 1019-1026 (1999).
8. Dunn, A. and Richards-Kortum, R., "Three-dimensional computation of light scattering from cells", IEEE J. Sel. Top. Quantum Electron., 2 (4), 898-905 (1996).
9. Perelman, L. T., Backman, V., Wallace, M., Zonios, G., Manoharan, R., Nusrat, A., Shields, S., Seiler, M., Lima, C., Hamano, T., Itzkan, I., Van Dam, J., Crawford, J. M. and Feld, M. S., "Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution", Phys. Rev. Lett., 80 (3), 627-630 (1998). Proc. of SPIE Vol. 7386 738602-7
10. Drezek, R., Guillaud, M., Collier, T., Boiko, I., Malpica, A., Macaulay, C., Follen, M. and Richards-Kortum, R., "Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture", J. Biomed. Opt., 8 (1), 7-16 (2003).
11. Wilson, J. D., Bigelow, C. E., Calkins, D. J. and Foster, T. H., "Light scattering from intact cells reports oxidativestress- induced mitochondrial swelling", Biophys. J., 88 (4), 2929-2938 (2005).
12. Su, X. T., Singh, K., Capjack, C., Petracek, J., Backhouse, C. and Rozmus, W., "Measurements of light scattering in an integrated microfluidic waveguide cytometer", J. Biomed. Opt., 13 (2), (2008).
13. Mie, G., "Articles on the optical characteristics of turbid tubes, especially colloidal metal solutions", Ann. Phys.- Berlin, 25 (3), 377-445 (1908).
14. Lu, J. Q., Yang, P. and Hu, X. H., "Simulations of light scattering from a biconcave red blood cell using the finitedifference time-domain method", J. Biomed. Opt., 10 (2), 024022 (2005).
15. Taflove, A., [Computational electrodynamics: the finite-difference time-domain method], Artech House Inc., Boston (1995).