Antigen detection at atomolar concentration using an optical tweezers

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7386, Issue , 2009, Pages

  Laliberté, Mathieu ^a   Bordeleau, François ^a,b   Marceau, Normand ^b   Sheng, Yunlong ^a  

^a UNIVERSITÉ LAVAL   (Canada)

^b LAVAL UNIVERSITY   (Canada)

Author keywords

Antigen antibody binding; Immunodetection; Optical tweezers

Indexed keywords

ANTIGEN-ANTIBODY BINDING; BINDING STRENGTH; BIOLOGICAL FLUIDS; BOVINE SERUM ALBUMINS; CANCER CELLS; CANCER DIAGNOSIS; CLINICAL ENVIRONMENTS; CONCENTRATION OF; IMMUNODETECTION; POLYSTYRENE BEADS; PROTEIN CONCENTRATIONS; PROTEIN FRAGMENTS; PROTEIN MARKERS; SENSITIVITY RANGE;

AMPLIFICATION; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BODY FLUIDS; CONCENTRATION (PROCESS); OPTICAL INSTRUMENTS; POLYSTYRENES;

OPTICAL TWEEZERS;

EID: 70449585743     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.839769     Document Type: Conference Paper

References (6)

1. Kramer G, Erdal H, Mertens HJ, et al. Differentiation between cell deathmodes using measurements of different soluble forms of extracellular cytokeratin 18. Cancer Res 2004;64:1751-6.
2. Goldsby, R.A., Kindt, T.J., Osborne, B.A. & Kuby, J., Antigen-Antibody Interactions. In: Immunology, 4th ed.(2000), p. 155. W. H. Freeman, New York.
3. Demiray M, Ulukaya EE, Arslan M, et al. Response to neoadjuvant chemotherapy in breast cancer could be predictable by measuring a novel serum apoptosis product, caspase-cleaved cytokeratin 18: a prospective pilot study. Cancer Invest 2006;24: 669-76.
4. Olofsson MH, Ueno T, Pan Y, et al. Cytokeratin-18 is a useful serumbiomarker for early determination of response of breast carcinomas to chemotherapy. Clin Cancer Res 2007;13:3198-206.
5. Helmerson K., Kishore R., D. Phillips W., H. Weetall H., Optical tweezers-based immunosensor detects femtomolar concentrations of antigens, Clinical Chemistry, 1997 43:2, 379-383.
6. Florin, E.L., et al, " Photonic force microscope calibration by thermal noise analysis" . Applied Physics A: Materials Science & Processing, 1998. 66(0): p. S75-S78.