High-throughput liquid-liquid extraction and LCMSMS assay for determination of circulating 25(OH) vitamin D3 and D2 in the routine clinical laboratory

Clinica Chimica Acta

Volumn 411, Issue 1-2, 2010, Pages 114-116

  Højskov, Carsten S ^a   Heickendorff, Lene ^a   Møller, Holger J ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

25 hydroxyvitamin D; Diatomaceous earth; Liquid liquid extraction; Tandem mass spectrometry

Indexed keywords

25 HYDROXYERGOCALCIFEROL; ACETONITRILE; CALCIFEDIOL; HEPTANE; INFUSORIAL EARTH;

ACCURACY; ARTICLE; CLINICAL LABORATORY; CONTROLLED STUDY; DENATURATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; LIQUID LIQUID EXTRACTION; PRIORITY JOURNAL; QUADRUPOLE MASS SPECTROMETRY; SERUM; TANDEM MASS SPECTROMETRY;

25-HYDROXYVITAMIN D 2; CALCIFEDIOL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; LIMIT OF DETECTION; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 70449532977     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2009.10.010     Document Type: Article

References (10)

1. Norman A.W. From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health. Am J Clin Nutr 88 (2008) 491S-499S
2. Zerwekh J.E. Blood biomarkers of vitamin D status. Am J Clin Nutr 87 (2008) 1087S-1091S
3. Hollis B.W., and Horst R.L. The assessment of circulating 25(OH)D and 1, 25(OH)2D: where we are and where we are going. J Steroid Biochem Mol Biol 103 (2007) 473-476
4. Roth H.J., Schmidt-Gayk H., Weber H., and Niederau C. Accuracy and clinical implications of seven 25-hydroxyvitamin D methods compared with liquid chromatography-tandem mass spectrometry as a reference. Ann Clin Biochem 45 (2008) 153-159
5. Vogeser M., Kyriatsoulis A., Huber E., and Kobold U. Candidate reference method for the quantification of circulating 25-hydroxyvitamin D3 by liquid chromatography-tandem mass spectrometry. Clin Chem 50 (2004) 1415-1417
6. Tsugawa N., Suhara Y., Kamao M., and Okano T. Determination of 25-hydroxyvitamin D in human plasma using high-performance liquid chromatography-tandem mass spectrometry. Anal Chem 77 (2005) 3001-3007
7. Taylor R.L., Grebe S.K., and Singh R.J. High throughput analysis of 25-hydroxyvitamin D2 & D3 by LC-MS/MS using an automated on-line extraction. Clin Chem 51 (2005) A231-A232
8. Maunsell Z., Wright D.J., and Rainbow S.J. Routine isotope-dilution liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of the 25-hydroxy metabolites of vitamins D2 and D3. Clin Chem 51 (2005) 1683-1690
9. Saenger A.K., Laha T.J., Bremner D.E., and Sadrzadeh S.M. Quantification of serum 25-hydroxyvitamin D(2) and D(3) using HPLC-tandem mass spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency. Am J Clin Pathol 125 (2006) 914-920
10. Chen H., McCoy L.F., Schleicher R.L., and Pfeiffer C.M. Measurement of 25-hydroxyvitamin D(3) (25OHD(3)) and 25-hydroxyvitamin D(2) (25OHD(2)) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method. Clin Chim Acta 391 (2008) 6-12