Live cell imaging with protein domains capable of recognizing phosphatidylinositol 4,5-bisphosphate; a comparative study

BMC Cell Biology

Volumn 10, Issue , 2009, Pages 67-

  Szentpetery, Zsofia ^a   Balla, Andras ^b   Kim, Yeun J ^a   Lemmon, Mark A ^c   Balla, Tamas ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b SEMMELWEIS UNIVERSITY   (Hungary)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHOLIPASE C; PLECKSTRIN;

ACCURACY; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL MEMBRANE; CELL STRAIN COS7; CELL STRAIN HEK293; CONTROLLED STUDY; CYTOLOGY; EMBRYO; ENZYME ACTIVATION; HUMAN; HUMAN CELL; MAMMAL; MEMBRANE BINDING; MEMBRANE STRUCTURE; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SENSITIVITY ANALYSIS; SEQUENCE HOMOLOGY; YEAST CELL;

ANIMALS; CALCIUM SIGNALING; CELL LINE; CELL SURVIVAL; CERCOPITHECUS AETHIOPS; ENZYME ACTIVATION; GENES, REPORTER; HUMANS; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTEINS;

EUKARYOTA; MAMMALIA;

EID: 70449413105     PISSN: None     EISSN: 14712121     Source Type: Journal    
DOI: 10.1186/1471-2121-10-67     Document Type: Article

References (38)

1. Berridge MJ. Inositol trisphosphate and diacylglycerol as intracellular messengers. Bio chem J 1984, 220:345-360.
2. Di Paolo G, De Camilli P. Phosphoinositides in cell regulation and membrane dynamics. Nature 2006, 443(7112):651-657. 10.1038/nature05185, 17035995.
3. Suh BC, Hille B. Regulation of ion channels by phosphatidylinositol 4,5-bisphosphate. Curr Opin Neurobiol 2005, 15:370-378. 10.1016/j.conb.2005.05.005, 15922587.
4. Hilgemann DW, Feng S, Nasuhoglu C. The complex and intriguing lives of PIP2 with ion channels and transporters. Sci STKE 2001, 2001(111):RE19. 10.1126/stke.2001.111.re19, 11734659.
5. Kolsch V, Charest PG, Firtel RA. The regulation of cell motility and chemotaxis by phospholipid signaling. J Cell Sci 2008, 121(Pt 5):551-559. 10.1242/jcs.023333, 2671295, 18287584.
6. Raucher D, Stauffer T, Chen W, Shen K, Guo S, York JD, Sheetz MP, Meyer T. Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. Cell 2000, 100:221-228. 10.1016/S0092-8674(00)81560-3, 10660045.
7. Irvine RF. Nuclear inositide signalling -- expansion, structures and clarification. Biochim Biophys Acta 2006, 1761(5-6):505-508. 1486819, 16574480.
8. Stauffer TP, Ahn S, Meyer T. Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr Biol 1998, 8:343-346. 10.1016/S0960-9822(98)70135-6, 9512420.
9. Várnai P, Balla T. Visualization of phosphoinositides that bind pleckstrin homology domains: calcium-and agonist-induced dynamic changes and relationship to myo-[3H]inositol-labeled phosphoinositide pools. J Cell Biol 1998, 143:501-510. 10.1083/jcb.143.2.501, 2132833, 9786958.
10. Varnai P, Balla T. Live cell imaging of phosphoinositide dynamics with fluorescent protein domains. Biochim Biophys Acta 2006, 1761(8):957-967.
11. Brown FD, Rozelle AL, Yin HL, Balla T, Donaldson JG. Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic. J Cell Biol 2001, 154:1007-1017. 10.1083/jcb.200103107, 2196179, 11535619.
12. Watt SA, Kular G, Fleming IN, Downes CP, Lucocq JM. Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C delta1. Bio chem J 2002, 363:657-666.
13. Hirose K, Kadowaki S, Tanabe M, Takeshima H, Iino M. Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns. Science 1999, 284:1527-1530. 10.1126/science.284.5419.1527, 10348740.
14. Nash MS, Young KW, Willars GB, Challiss RA, Nahorski SR. Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation. Bio chem J 2001, 356(Pt 1):137-142.
15. Lemmon MA. Pleckstrin homology domains: not just for phosphoinositides. Biochem Soc Trans 2004, 32:707-711. 10.1042/BST0320707, 15493994.
16. Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray D, Emr SD, Lemmon MA. Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains. Mol Cell 2004, 13:677-688. 10.1016/S1097-2765(04)00083-8, 15023338.
17. Carroll K, Gomez C, Shapiro L. Tubby proteins: the plot thickens. Nat Rev Mol Cell Biol 2004, 5:55-63. 10.1038/nrm1278, 14708010.
18. Santagata S, Boggon TJ, Baird CL, Gomez CA, Zhao J, Shan WS, Myszka DG, Shapiro L. G-protein signaling through tubby proteins. Science 2001, 292:2041-2050. 10.1126/science.1061233, 11375483.
19. Field SJ, Madson N, Kerr ML, Galbraith KA, Kennedy CE, Tahiliani M, Wilkins A, Cantley LC. PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis. Curr Biol 2005, 15(15):1407-1412. 10.1016/j.cub.2005.06.059, 16085494.
20. Nelson CP, Nahorski SR, Challiss RA. Temporal profiling of changes in phosphatidylinositol 4,5-bisphosphate, inositol 1,4,5-trisphosphate and diacylglycerol allows comprehensive analysis of phospholipase C-initiated signalling in single neurons. J Neurochem 2008, 107(3):602-615. 10.1111/j.1471-4159.2008.05587.x, 18665913.
21. Quinn KV, Behe P, Tinker A. Monitoring changes in membrane phosphatidylinositol 4,5-bisphosphate in living cells using a domain from the transcription factor tubby. J Physiol 2008, 586(Pt 12):2855-2871. 10.1113/jphysiol.2008.153791, 2517197, 18420701.
22. Balla A, Ju Kim Y, Varnai P, Szentpetery Z, Knight Z, Shokat KM, Balla T. Maintenance of Hormone-sensitive Phosphoinositide Pools in the Plasma Membrane Requires Phosphatidylinositol 4-Kinase III{alpha}. Mol Biol Cell 2007, 19(2):711-721. 10.1091/mbc.E07-07-0713, 2230591, 18077555.
23. Varnai P, Thyagarajan B, Rohacs T, Balla T. Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J Cell Biol 2006, 175(3):377-382. 10.1083/jcb.200607116, 2064515, 17088424.
24. Wal J, Habets R, Varnai P, Balla T, Jalink K. Monitoring Phospholipase C activation kinetics in live cells by FRET. J Biol Chem 2001, 276:15337-15344. 10.1074/jbc.M007194200, 11152673.
25. Hammond GR, Sim Y, Lagnado L, Irvine RF. Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial information. J Cell Biol 2009, 184(2):297-308. 10.1083/jcb.200809073, 2654307, 19153221.
26. Balla A, Tuymetova G, Tsiomenko A, Varnai P, Balla T. A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. Mol Biol Cell 2005, 16:1282-1295. 10.1091/mbc.E04-07-0578, 551492, 15635101.
27. Varnai P, Lin X, Lee SB, Tuymetova G, Bondeva T, Spat A, Rhee SG, Hajnoczky G, Balla T. Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130. J Biol Chem 2002, 277:27412-27422. 10.1074/jbc.M109672200, 12019260.
28. Uchiyama T, Yoshikawa F, Hishida A, Furuichi T, Mikoshiba K. A novel recombinant hyper-affinity Inositol 1,4,5-trisphosphate (IP3) absorbent traps IP3, resulting in specific inhibition of IP3-mediated calcium signaling. J Biol Chem 2001, 277:8106-8113. 10.1074/jbc.M108337200, 11741904.
29. Varnai P, Balla A, Hunyady L, Balla T. Targeted expression of the inositol 1,4,5-triphosphate receptor (IP3R) ligand-binding domain releases Ca2+ via endogenous IP3R channels. Proc Natl Acad Sci USA 2005, 102(22):7859-7864. 10.1073/pnas.0407535102, 1142351, 15911776.
30. Xu C, Watras J, Loew LM. Kinetic analysis of receptor-activated phosphoinositide turnover. J Cell Biol 2003, 161:779-791. 10.1083/jcb.200301070, 2199375, 12771127.
31. Bartlett PJ, Young KW, Nahorski SR, Challiss RA. Single cell analysis and temporal profiling of agonist-mediated inositol 1,4,5-trisphosphate, Ca2+, diacylglycerol, and protein kinase C signaling using fluorescent biosensors. J Biol Chem 2005, 280(23):21837-21846. 10.1074/jbc.M411843200, 15788407.
32. Roy A, Levine TP. Multiple pools of phosphatidylinositol 4-phosphate detected using the pleckstrin homology domain of Osh2p. J Biol Chem 2004, 279:44683-44689. 10.1074/jbc.M401583200, 15271978.
33. Yeung T, Terebiznik M, Yu L, Silvius J, Abidi WM, Philips M, Levine T, Kapus A, Grinstein S. Receptor activation alters inner surface potential during phagocytosis. Science 2006, 313(5785):347-351. 10.1126/science.1129551, 16857939.
34. Shi X, Basran J, Seward HE, Childs W, Bagshaw CR, Boxer SG. Anomalous negative fluorescence anisotropy in yellow fluorescent protein (YFP 10C): quantitative analysis of FRET in YFP dimers. Biochemistry 2007, 46(50):14403-14417. 10.1021/bi701575n, 2570256, 18027983.
35. Nash MS, Willets JM, Billups B, John Challiss RA, Nahorski SR. Synaptic activity augments muscarinic acetylcholine receptor-stimulated inositol 1,4,5-trisphosphate production to facilitate Ca2+ release in hippocampal neurons. J Biol Chem 2004, 279(47):49036-49044. 10.1074/jbc.M407277200, 15342646.
36. Nash MS, Schell MJ, Atkinson PJ, Johnston NR, Nahorski SR, Challiss RA. Determinants of metabotropic glutamate receptor-5-mediated Ca2+ and inositol 1,4,5-trisphosphate oscillation frequency. Receptor density versus agonist concentration. J Biol Chem 2002, 277(39):35947-35960. 10.1074/jbc.M205622200, 12119301.
37. Watras J, Fink CC, Loew LM. Endogenous inhibitors of InsP3-induced Ca2+ release in neuroblastoma cells. Brain Res 2005, 1055(1-2):60-72. 10.1016/j.brainres.2005.06.091, 16095574.
38. Horowitz LF, Hirdes W, Suh BC, Hilgemann DW, Mackie K, Hille B. Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current. J Gen Physiol 2005, 126(3):243-262. 10.1085/jgp.200509309, 2266577, 16129772.