Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate

Journal of Bioscience and Bioengineering

Volumn 108, Issue 5, 2009, Pages 359-364

  Sahara, Hiroshi ^a   Kotaka, Atsushi ^a   Kondo, Akihiko ^b   Ueda, Mitsuyoshi ^c   Hata, Yoji ^a  

^a Gekkeikan Sake Company Ltd   (Japan)

^b KOBE UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Alcohol acetyltransferase; HELOH method; Industrial diploid yeast; Loss of heterozygosity; Sake yeast; SED1 promoter

Indexed keywords

ACETYL TRANSFERASE; GENETIC MODIFICATIONS; HELOH METHOD; HETEROZYGOSITY; HIGH EFFICIENCY; INDUSTRIAL DIPLOID YEAST; INDUSTRIAL SCALE; ISOAMYL ACETATE; SAKE BREWING; SAKE YEAST; SAKE YEASTS; SED1 PROMOTER; YEAST STRAIN;

FERMENTATION; GENETIC ENGINEERING; INDUSTRIAL APPLICATIONS;

YEAST;

ALCOHOL; AMINO ACID; GLUCOSE; ISOPENTYL ACETATE;

ANIMAL CELL; ARTICLE; BREWING; CONTROLLED STUDY; DIPLOIDY; DNA MODIFICATION; FERMENTATION; FUNGAL STRAIN; GENE DISRUPTION; GENE OVEREXPRESSION; GENETIC ENGINEERING; GENETIC STABILITY; HETEROZYGOSITY LOSS; HOMOZYGOSITY; MATING; NONHUMAN; PROMOTER REGION; SPOROGENESIS;

ACETYLTRANSFERASES; AMINO ACID SUBSTITUTION; ETHANOL; GENETIC ENHANCEMENT; INDUSTRIAL MICROBIOLOGY; LOSS OF HETEROZYGOSITY; MEMBRANE GLYCOPROTEINS; PENTANOLS; PROMOTER REGIONS, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SELECTION, GENETIC; UP-REGULATION; WINE;

EID: 70449344589     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.05.002     Document Type: Article

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