Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific lipid-transfer proteins

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 68-75

  Pokoj, Sven ^a   Lauer, Iris ^a   Fötisch, Kay ^a   Himly, Martin ^b   Mari, Adriano ^c   Enrique, Ernesto ^d   Miguel Moncin, Maria del Mar San ^e   Lidholm, Jonas ^f   Vieths, Stefan ^a   Scheurer, Stephan ^a  

^a PAUL EHRLICH INSTITUT   (Germany)

^b UNIVERSITY OF SALZBURG   (Austria)

^c LABORATORIO DI PATOLOGIA VASCOLARE   (Italy)

^d HOSPITAL GENERAL DE CASTELLÓN   (Spain)

^e Pius Hospital de Valls   (Spain)

^f Thermo Fisher Scientific   (United States)

Author keywords

Cor a 8; Hazelnut; Heterologous over expression; Non specific lipid transfer proteins; nsLTP; Recombinant allergen

Indexed keywords

ALLERGEN; CARRIER PROTEIN; COR A 8 ALLERGEN, CORYLUS AVELLANA; IMMUNOGLOBULIN E; LIPID TRANSFER PROTEIN; PLANT ANTIGEN; RECOMBINANT PROTEIN;

ARTICLE; BIOASSAY; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; HISTAMINE RELEASE; HUMAN; IMMUNOBLOTTING; IMMUNOLOGY; METABOLISM; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; REPRODUCIBILITY; SACCHAROMYCES CEREVISIAE;

ALLERGENS; ANTIGENS, PLANT; BIOLOGICAL ASSAY; CARRIER PROTEINS; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTAMINE RELEASE; HUMANS; IMMUNOBLOTTING; IMMUNOGLOBULIN E; PICHIA; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE;

BACTERIA (MICROORGANISMS); CORYLUS; ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 70449127064     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.08.014     Document Type: Article

References (40)

1. Steckelbroeck S., Ballmer-Weber B.K., and Vieths S. Potential, pitfalls, and prospects of food allergy diagnostics with recombinant allergens or synthetic sequential epitopes. J. Allergy Clin. Immunol. 121 (2008) 1323-1330
2. Pastorello E.A., and Robino A.M. Clinical role of lipid transfer proteins in food allergy. Mol. Nutr. Food Res. 48 (2004) 356-362
3. Schocker F., Luttkopf D., Scheurer S., Petersen A., Cistero-Bahima A., Enrique E., Miguel-Moncin M., Akkerdaas J., van Ree R., Vieths S., and Becker W.M. Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy. J. Allergy Clin. Immunol. 113 (2004) 141-147
4. Kader J.C. Lipid-transfer proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996) 627-654
5. Asero R., Mistrello G., Roncarolo D., de Vries S.C., Gautier M.F., Ciurana C.L., Verbeek E., Mohammadi T., Knul-Brettlova V., Akkerdaas J.H., Bulder I., Aalberse R.C., and van Ree R. Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. Int. Arch. Allergy Immunol. 124 (2001) 67-69
6. Sancho A.I., Rigby N.M., Zuidmeer L., Asero R., Mistrello G., Amato S., Gonzalez-Mancebo E., Fernandez-Rivas M., van Ree R., and Mills E.N. The effect of thermal processing on the IgE reactivity of the non-specific lipid transfer protein from apple, Mal d 3. Allergy 60 (2005) 1262-1268
7. Scheurer S., Lauer I., Foetisch K., Moncin M.S., Retzek M., Hartz C., Enrique E., Lidholm J., Cistero-Bahima A., and Vieths S. Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion. J. Allergy Clin. Immunol. 114 (2004) 900-907
8. Pastorello E.A., Pompei C., Pravettoni V., Farioli L., Calamari A.M., Scibilia J., Robino A.M., Conti A., Iametti S., Fortunato D., Bonomi S., and Ortolani C. Lipid-transfer protein is the major maize allergen maintaining IgE-binding activity after cooking at 100 degrees C, as demonstrated in anaphylactic patients and patients with positive double-blind, placebo-controlled food challenge results. J. Allergy Clin. Immunol. 112 (2003) 775-783
9. Pastorello E.A., Vieths S., Pravettoni V., Farioli L., Trambaioli C., Fortunato D., Lüttkopf D., Calamari M., Ansaloni R., Scibilia J., Ballmer-Weber B.K., Poulsen L.K., Wüthrich B., Hansen K.S., Robino A.M., Ortolani C., and Conti A. Identification of hazelnut major allergens in sensitive patients with positive double-blind, placebo-controlled food challenge results. J. Allergy Clin. Immunol. 109 (2002) 563-570
10. Niederberger V., and Valenta R. Recombinant allergens for immunotherapy. Where do we stand?. Curr. Opin. Allergy Clin. Immunol. 4 (2004) 549-554
11. Enrique E., Pineda F., Malek T., Bartra J., Basagana M., Tella R., Castello J.V., Alonso R., de Mateo J.A., Cerda-Trias T., Miguel-Moncin M.M., Monzon S., Garcia M., Palacios R., and Cistero-Bahima A. Sublingual immunotherapy for hazelnut food allergy: a randomized, double-blind, placebo-controlled study with a standardized hazelnut extract. J. Allergy Clin. Immunol. 116 (2005) 1073-1079
12. Enrique E., Malek T., Pineda F., Palacios R., Bartra J., Tella R., Basagana M., Alonso R., and Cistero-Bahima A. Sublingual immunotherapy for hazelnut food allergy: a follow-up study. Ann. Allergy Asthma Immunol. 100 (2008) 283-284
13. Spangfort M.D., and Larsen J.N. Standardization of allergen-specific immunotherapy vaccines. Immunol. Allergy Clin. North Am. 26 (2006) 191 1vi
14. Lauer I., Miguel-Moncin M.S., Abel T., Foetisch K., Hartz C., Fortunato D., Cistero-Bahima A., Vieths S., and Scheurer S. Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3. Clin. Exp. Allergy 37 (2007) 261-269
15. Rigby N.M., Marsh J., Sancho A.I., Wellner K., Akkerdaas J., van Ree R., Knulst A., Fernandez-Rivas M., Brettlova V., Schilte P.P., Summer C., Pumphrey R., Shewry P.R., and Mills E.N. The purification and characterisation of allergenic hazelnut seed proteins. Mol. Nutr. Food Res. 52 Suppl. 2 (2008) S251-S261
16. Duro G., Colombo P., Assunta C.M., Izzo V., Porcasi R., Di Fiore R., Locorotondo G., Cocchiara R., and Geraci D. Isolation and characterization of two cDNA clones coding for isoforms of the Parietaria judaica major allergen Par j 1.0101. Int. Arch. Allergy Immunol. 112 (1997) 348-355
17. Scheurer S., Pastorello E.A., Wangorsch A., Kastner M., Haustein D., and Vieths S. Recombinant allergens Pru av 1 and Pru av 4 and a newly identified lipid transfer protein in the in vitro diagnosis of cherry allergy. J. Allergy Clin. Immunol. 107 (2001) 724-731
18. Le L.Q., Lorenz Y., Scheurer S., Fotisch K., Enrique E., Bartra J., Biemelt S., Vieths S., and Sonnewald U. Design of tomato fruits with reduced allergenicity by dsRNAi-mediated inhibition of ns-LTP (Lyc e 3) expression. Plant Biotechnol. J. 4 (2006) 231-242
19. Lauber T., Marx U.C., Schulz A., Kreutzmann P., Rosch P., and Hoffmann S. Accurate disulfide formation in Escherichia coli: overexpression and characterization of the first domain (HF6478) of the multiple Kazal-type inhibitor LEKTI. Protein Expr. Purif. 22 (2001) 108-112
20. Yadava A., and Ockenhouse C.F. Effect of codon optimization on expression levels of a functionally folded malaria vaccine candidate in prokaryotic and eukaryotic expression systems. Infect. Immun. 71 (2003) 4961-4969
21. Schmidt M., and Hoffman D.R. Expression systems for production of recombinant allergens. Int. Arch. Allergy Immunol. 128 (2002) 264-270
22. Huecas S., Villalba M., Gonzalez E., Martinez-Ruiz A., and Rodriguez R. Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris. Eur. J. Biochem. 261 (1999) 539-546
23. Hiller K.M., Lubahn B.C., and Klapper D.G. Cloning and expression of ragweed allergen Amb a 6. Scand. J. Immunol. 48 (1998) 26-36
24. Ahrazem O., Ibanez M.D., Lopez-Torrejon G., Sanchez-Monge R., Sastre J., Lombardero M., Barber D., and Salcedo G. Lipid transfer proteins and allergy to oranges. Int. Arch. Allergy Immunol. 137 (2005) 201
25. Zuidmeer L., Salentijn E., Rivas M.F., Mancebo E.G., Asero R., Matos C.I., Pelgrom K.T., Gilissen L.J., and Van Ree R. The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. Clin. Exp. Allergy 36 (2006) 666-675
26. Diaz-Perales A., Garcia-Casado G., Sanchez-Monge R., Garcia-Selles F.J., Barber D., and Salcedo G. CDNA cloning and heterologous expression of the major allergens from peach and apple belonging to the lipid-transfer protein family. Clin. Exp. Allergy 32 (2002) 87-92
27. Klein C., Lamotte-Guery F., Gautier F., Moulin G., Boze H., Joudrier P., and Gautier M.F. High-level secretion of a wheat lipid transfer protein in Pichia pastoris. Protein Expr. Purif. 13 (1998) 73-82
28. Asero R., Mistrello G., Roncarolo D., de Vries S.C., Gautier M.F., Ciurana C.L., Verbeek E., Mohammadi T., Knul-Brettlova V., Akkerdaas J.H., Bulder I., Aalberse R.C., and van Ree R. Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. Int. Arch. Allergy Immunol. 122 (2000) 20-32
29. Lauer I., Alessandri S., Pokoj S., Reuter A., Conti A., Vieths S., and Scheurer S. Expression and characterization of three important panallergens from hazelnut. Mol. Nutr. Food Res. 52 Suppl. 2 (2008) S262-S271
30. Dreborg S., and Frew A. Position paper: allergen standardization and skin tests. The European academy of allergology and clinical immunology. Allergy 48 (1993) 48-82
31. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
32. Marknell-DeWitt D.A., Niederberger V., Lehtonen P., Spitzauer S., Sperr W.R., Valent P., Valenta R., and Lidholm J. Molecular and immunological characterization of a novel timothy grass (Phleum pratense) pollen allergen, Phl p 11. Clin. Exp. Allergy 32 (2002) 1329-1340
33. Kleine-Budde B., I, de Heer P.G., van der Zee J.S., and Aalberse R.C. The stripped basophil histamine release bioassay as a tool for the detection of allergen-specific IgE in serum. Int. Arch. Allergy Immunol. 126 (2001) 277-285
34. Foetisch K., Westphal S., Lauer I., Retzek M., Altmann F., Kolarich D., Scheurer S., and Vieths S. Biological activity of IgE specific for cross-reactive carbohydrate determinants. J. Allergy Clin. Immunol. 111 (2003) 889-896
35. Lauer I., Foetisch K., Kolarich D., Ballmer-Weber B.K., Conti A., Altmann F., Vieths S., and Scheurer S. Hazelnut (Corylus avellana) vicilin Cor a 11: molecular characterization of a glycoprotein and its allergenic activity. Biochem. J. 383 (2004) 327-334
36. Cregg J.M., Madden K.R., Barringer K.J., Thill G.P., and Stillman C.A. Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol. Cell Biol. 9 (1989) 1316-1323
37. Breiteneder H., and Radauer C. A classification of plant food allergens. J. Allergy Clin. Immunol. 113 (2004) 821-830
38. Vailes L.D., Kinter M.T., Arruda L.K., and Chapman M.D. High-level expression of cockroach allergen, Bla g 4, in Pichia pastoris. J. Allergy Clin. Immunol. 101 (1998) 274-280
39. Hartz C., Miguel-Moncin M.M., Cistero-Bahima A., Fotisch K., Metzner K.J., Fortunato D., Lidholm J., Vieths S., and Scheurer S. Molecular characterisation of Lac s 1, the major allergen from lettuce (Lactuca sativa). Mol. Immunol. 44 (2007) 2820-2830
40. Pasquato N., Berni R., Folli C., Folloni S., Cianci M., Pantano S., Helliwell J.R., and Zanotti G. Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens. J. Mol. Biol. 356 (2006) 684-694