Optimization and comparison of bacterial load and sampling time for bioaerosol detection systems in a poultry layer house

Journal of Applied Poultry Research

Volumn 13, Issue 3, 2004, Pages 433-442

  Woodward, C L ^a   Park, S Y ^a   Jackson, D R ^a   Li, X ^a   Birkhold, S G ^a   Pillai, S D ^a   Ricke, S C ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Aerosol detection; Airborne bacteria; Poultry aerosols

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 7044247568     PISSN: 10566171     EISSN: None     Source Type: Journal    
DOI: 10.1093/japr/13.3.433     Document Type: Article

References (51)

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25. 3)/min.
26. Puralator Inc., Henderson, NV.
27. Uniek, Waunakee, WI.
28. Lakewood Eng and Mfg., Chicago, IL.
29. Extech Instrument Corp., Walthman, MA.
30. For total bacterial colony-forming units, the samples were brought to the laboratory within an hour after sampling. To each bioaerosol sample, 20 mL of 2% peptone (time frame analysis of airborne samplers) or 0.1% peptone (comparison of airborne samplers, except for the drag-swab samples, which were 40 mL of 0.1%) was added and stomached for 30 s. A 0.5-mL aliquot of the original dilution was serially diluted in peptone for plating. Tryptic soy agar [31] was used to enumerate total aerobic bacteria. Plates were incubated for 48 h at 37°C.
31. Tryptic Soy Agar, TSA; Difco, Detroit, MI.
32. 3)/min.
33. AGI-30, Ace Glass Inc., Vineland, NJ.
34. 3)/min.
35. Anderson Instr., Smyrna, Georgia.
36. Lakewood Eng and Mfg., Chicago, IL.
37. 3)/min.
38. Drag-swab assemblies were made according to previously published methods with minor alterations in materials [51, 14, 17]. Drag-swab assemblies were made from 2, 4 x 4 in (10.2 x 10.2 cm) sterile cotton cheese cloth [39] and 6-ft (182. 9 cm) length of size 18 cotton twine [40]. The gauze pads were securely tied at 5 ft (152.4 cm) and 6 ft (182. 9 cm) on the cotton twine. Each assembly was placed in a glass bottle and autoclaved. Each assembly was wetted in 20 mL of 0.1% peptone and dragged the entire length of the poultry layer house 4 times in separate vertical lines.
39. American Fiber and Finishing Inc., Burlington, MA.
40. Ace Hardware, Oakbrook, IL.
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43. SPSS for Windows, standard version 10.0, SPSS, Inc., Chicago, IL.
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