An automated image capture and quantitation approach to identify proteins affecting tumor cell proliferation

Journal of Biomolecular Screening

Volumn 9, Issue 3, 2004, Pages 216-222

  Bhawe, Kaumudi M ^a,b   Blake, Robert A ^a   Clary, Douglas O ^a   Flanagan, Peter M ^a  

^a SUGEN INC   (United States)

^b EXELIXIS INC   (United States)

Author keywords

BrdU; Cellomics; Immunofluorescence; Proliferation

Indexed keywords

APHIDICOLIN; CYCLIN DEPENDENT KINASE INHIBITOR; PROTEIN P27; PROTEIN P53; PROTEIN SERINE THREONINE KINASE; TUMOR SUPPRESSOR PROTEIN; BROXURIDINE; TUMOR PROTEIN;

ALLELE; ARTICLE; AUTOMATION; CELL CYCLE S PHASE; CELL PROLIFERATION; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VITRO STUDY; LUNG CARCINOMA; MELANOMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; TECHNOLOGY; TUMOR CELL CULTURE; CARCINOMA; CELL CULTURE; CELL DIVISION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; LUNG TUMOR; METABOLISM; METHODOLOGY; NEOPLASM; PATHOLOGY; PHYSIOLOGY; SENSITIVITY AND SPECIFICITY;

AUTOMATION; BROMODEOXYURIDINE; CARCINOMA; CELL DIVISION; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; LUNG NEOPLASMS; MELANOMA; NEOPLASM PROTEINS; NEOPLASMS; SENSITIVITY AND SPECIFICITY; TUMOR CELLS, CULTURED;

EID: 7044231365     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103262842     Document Type: Article

References (7)

1. Cunningham BA: A growing issue: cell proliferation assays. The Scientist 2001;15(13):26.
2. Jian Y, Van Tine BA, Chien WM, Shaw GM, Broker TR, Chow LT: Concordant induction of cyclin E and p21cip1 in differentiated keratinocytes by the human papillomavirus E7 protein inhibits cellular and viral DNA synthesis. Cell Growth Differ 1999;10(2):101-111.
3. Ghosh RN, Chen YT, DeBiasio R, DeBiasio RL, Conway BR, Minor LK, et al: Cell-based, high-content screen for receptor internalization, recycling and intracellular trafficking. Biotechniques 2000;29(1):170-175.
4. Ding GJ, Fischer PA, Boltz RC, Schmidt JA, Colaianne JJ, Gough A, et al: Characterization and quantitation of NF-kappaB nuclear translocation induced by interleukin-1 and tumor necrosis factor-alpha: development and use of a high capacity fluorescence cytometric system. J Biol Chem 1998;273(44):28897-28905.
5. Craig C, Wersto R, Kim M, Ohri E, Li Z, Katayose D, et al: A recombinant adenovirus expressing p27Kip1 induces cell cycle arrest and loss of cyclin-Cdk activity in human breast cancer cells. Oncogene 1997;14(19):2283-2289.
6. Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, et al: A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J 1998;17:3052-3065.
7. Ma L, Bluyssen HA, De Raeymaeker M, Laurysens V, van der Beek N, Pavliska H, et al: Rapid determination of adenoviral vector titers by quantitative real-time PCR. J Virol Methods 2001;93:181-188.