Probing the unfolding region of ribonuclease A by site-directed mutagenesis

European Journal of Biochemistry

Volumn 271, Issue 20, 2004, Pages 4147-4156

  Köditz, Jens ^a   Ulbrich Hofmann, Renate ^a   Arnold, Ulrich ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Limited proteolysis; Local unfolding; Protein engineering; Ribonuclease A; Stability

Indexed keywords

ALANINE; ARGININE; ASPARAGINE; ASPARTIC ACID; LEUCINE; LYSINE; PHENYLALANINE; PROLINE; PROTEINASE; RIBONUCLEASE A; SERINE; THERMOLYSIN; TRYPSIN; TYROSINE;

ARTICLE; BETA SHEET; CATALYSIS; ENZYME CONFORMATION; ENZYME STABILITY; HYDROGEN BOND; HYDROPHOBICITY; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

AMINO ACID SUBSTITUTION; AMINO ACIDS; CIRCULAR DICHROISM; ESCHERICHIA COLI; GUANIDINE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGONUCLEOTIDES; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RIBONUCLEASE, PANCREATIC; SOLVENTS; TEMPERATURE; THERMODYNAMICS; THERMOLYSIN; TRANSFORMATION, GENETIC;

EID: 7044223181     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04355.x     Document Type: Article

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