Conjunctival epithelial cells maintain stem cell properties after long-term culture and cryopreservation

Regenerative Medicine

Volumn 4, Issue 5, 2009, Pages 677-687

  Schrader, Stefan ^a,b   Notara, M ^a   Beaconsfield, M ^c   Tuft, S ^c   Geerling, G ^d   Daniels, J T ^a,c  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF LÜBECK   (Germany)

^c MOORFIELDS EYE HOSPITAL   (United Kingdom)

^d UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Cell therapy; Conjunctiva; In vitro culture; Stem cell; Tissue engineering

Indexed keywords

BREAST CANCER RESISTANCE PROTEIN; CYTOKERATIN 19; CYTOKERATIN 4; LIQUID NITROGEN; PROTEIN P63; PROTEIN P63 ALPHA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL AGING; CELL CULTURE; CELL GROWTH; CELL ISOLATION; CELL POPULATION; CELL PROLIFERATION; CELL STRAIN 3T3; CELL VIABILITY; COLONY FORMATION; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE TECHNIQUE; EPITHELIUM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL EXPANSION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; COCULTURE TECHNIQUES; CONJUNCTIVA; CRYOPRESERVATION; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; MIDDLE AGED; NIH 3T3 CELLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS; TIME FACTORS; TISSUE CULTURE TECHNIQUES; TISSUE ENGINEERING;

EID: 70350765265     PISSN: 17460751     EISSN: None     Source Type: Journal    
DOI: 10.2217/rme.09.39     Document Type: Article

References (22)

1. Lavker RM, Sun TT: Epithelial stem cells: the eye provides a vision. Eye 17, 937-942 (2003).
2. Ang LP, Tan DT, Cajucom-Uy H, Beuerman RW: Autologous cultivated conjunctival transplantation for pterygium surgery. Am. J. Ophthalmol. 139, 611-619 (2005).
3. Tan DT, Ang LP, Beuerman RW: Reconstruction of the ocular surface by transplantation of a serum-free derived cultivated conjunctival epithelial equivalent. Transplantation 77, 1729-1734 (2004).
4. De Bruyn C, Delforge A, Bernier M, Bron D: Ex vivo expansion of neutrophil precursor cells from fresh and cryopreserved cord blood cells. Cytotherapy 5, 87-98 (2003).
5. Polge C, Smith AU, Parkes AS: Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature 164, 666 (1949).
6. Oh JY, Kim MK, Shin KS et al.: Efficient cryopreservative conditions for cultivated limbal and conjunctival epithelial cells. Cornea 26, 840-846 (2007).
7. Yeh HJ, Yao CL, Chen HI, Cheng HC, Hwang SM: Cryopreservation of human limbal stem cells ex vivo expanded on amniotic membrane. Cornea 27, 327-333 (2008).
8. Kito K, Kagami H, Kobayashi C, Ueda M, Terasaki H: Effects of cryopreservation on histology and viability of cultured corneal epithelial cell sheets in rabbit. Cornea 24, 735-741 (2005).
9. Harkin DG, Barnard Z, Gillies P, Ainscough SL, Apel AJ: Analysis of p63 and cytokeratin expression in a cultivated limbal autograft used in the treatment of limbal stem cell deficiency. Br. J. Ophthalmol. 88, 1154-1158 (2004).
10. Rheinwald JG, Green H: Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6, 331-343 (1975).
11. Barrandon Y, Green H: Three clonal. types of keratinocyte with different capacities for multiplication. Proc. Natl Acad. Sci. USA 84, 2302-2306 (1987).
12. Freshney RI: Culture of Animal Cells (1st Edition). Wiley-Liss, NY, USA (2000).
13. Pellegrini G, Golisano O, Paterna P et al.: Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J. Cell Biol. 145, 769-782 (1999).
14. Kawasaki S, Tanioka H, Yamasaki K, Connon CJ, Kinoshita S: Expression and tissue distribution of p63 isoforms in human ocular surface epithelia. Exp. Eye Res. 82, 293-299 (2006).
15. Henderson HW, Collin JR: Mucous membrane grafting. Dev. Ophthalmol. 41, 230-242 (2008).
16. Armitage WJ: Cryopreservation of animal cells. Symp. Soc. Exp. Biol. 41, 379-393 (1987).
17. Taylor MJ, Hunt CJ: Tolerance of corneas to multimolar dimethyl sulfoxide at 0°C. Implications for cryopreservation. Invest. Ophthalmol. Vis. Sci. 30, 400-412 (1989).
18. Risse Marsh BC, Massaro-Giordano M, Marshall CM, Lavker RM, Jensen PJ: Initiation and characterization of keratinocyte cultures from biopsies of normal human conjunctiva. Exp. Eye Res. 74, 61-69 (2002).
19. Pellegrini G, Dellambra E, Golisano O et al.: p63 identifies keratinocyte stem cells. Proc. Natl Acad. Sci. USA 98, 3156-3161 (2001).
20. Budak MT, Alpdogan OS, Zhou M, Lavker RM, Akinci MA, Wolosin N: Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells. J Cell Sci. 118, 1715-1724 (2005).
21. Watanabe K, Nishida K, Yamato M et al.: Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG 2. FEBS Lett. 565, 6-10 (2004).
22. Tanioka H, Kawasaki S, Yamasaki K et al.: Establishment of a cultivated human conjunctival epithelium as an alternative tissue source for autologous corneal epithelial transplantation. Invest. Ophthalmol. Vis. Sci. 47, 3820-3827 (2006).