Comparison of PCR assay with serum and whole blood samples of experimental trials for detection and differentiation of brucella melitensis

Journal of Animal and Veterinary Advances

Volumn 8, Issue 8, 2009, Pages 1637-1640

  Takele, B Y ^a   Khairani Bejo, S ^a   Bahaman, A R ^a   Omar, A R ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Brucella; IS711; Mice; PCR; Serum; Whole blood

Indexed keywords

EID: 70350752741     PISSN: 16805593     EISSN: 1993601X     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Amin, A.S., H.S. Husseinen, G.S. Radwan, M.N. Shalaby andN. El-Danaf, 1995. The polymerase chain reaction assay as a rapid and sensitive test for detection of Brucella antigen in field samples. J. Energy Vet. Med. Assoc.,55: 761-767.
2. Bougnoux, M..E, C. Dupomt, J. Mateo, P. Saulnier, V. Faivre, D. Payene and M.H.N. Chanome, 1999. Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR. J. Clin. Microbiol., 37: 925-930.
3. Davidson, M., A. Shimshony, H. Adler, M. Banai, A. Cohen, 1990. Protection of Brucella free Areas from Re-Infection. In: Adams, L.G. (Ed.). Advances in Brucellosis Research. Texas A and M University Press, Texas, pp: 207-443.
4. Elfaki, M.G., T. Uz-Zaman, A.A. Al-Hokail, S.M. Nakeeb, 2005. Detection of Brucella DNA in sera from patients with brucellosis by polym erase chain reaction. Diagn. Microbiol. Infect. Dis., 53: 1-7.
5. Kawamura, S., S. Maesaki, T. Noda, Y. Hirakata, K. Tomono, T. Tashiro and S. Kohno, 1999. Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis. J. Clin. Microbiol., 37:218-200.
6. Leal-Klevezas, D.S., I.O. Martinez-Vazques, A. Lopez-Merino and J.P. Martinez Soriano, 1995. Single-step PCR for detection of Brucella sp. from blood and milk of infected animals. J. Clin. Microbiol., 33: 3087-3090.
7. Leyla, G., G. Kadri and O. Omran, 2003. Comparison of PCR and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet. Microbiol., 93: 53-61.
8. Morata, P., M.I. Queipo-Ortuno, J.D. Colmenero, 1998. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J. Clm. Microbiol., 6: 2443-2446.
9. Morris, T., B. Robertson and M. Gallagher, 1996. Rapid rivers transcription PCR detection of hepatitis C virus RNA in serum by using TaqMan flourogenic detection system. Journal of Clinical.
10. Queipo-Ortuno, M.I., J.D. Colmenero, M.J. Reguera, M.A. Garcia-Ordonez, M.E. Pachon, M. Gonzalez and P. Morata, 2004. Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. J. Clm. Microbiol. Infect., 11:713-718.
11. Redkar, R, S. Rose, B. J. Bncker and V. DelVecchio, 2001. Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis. Mol. Cell Probes, 15:43-52.
12. Sambrook, J.,E.F. Fritsh and T. Maniatis, 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Painview, New York.
13. Young, E.J., C.I. Gomez, D.H. Yawn and D.M. Musher, 1979. Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infect. Immun., 26 (2): 680-685.
14. Zerva, L., K. Bourantas, S. Mitka, A. Kansouzidou and N.J. Legakis, 2001. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J. Clm. Microbiol., 39: 1661-1664.