Hepatocyte differentiation from human ES cells using the simple embryoid body formation method and the staged-additional cocktail

TheScientificWorldJournal

Volumn 9, Issue , 2009, Pages 884-890

  Sasaki, Katsunori ^a   Ichikawa, Hinako ^a   Takei, Shunsuke ^a   No, Hee Sung ^a   Tomotsune, Daihachiro ^a   Kano, Yoshiya ^a   Yokoyama, Tadayuki ^b   Sirasawa, Sakiko ^b   Mogi, Akimi ^a   Yoshie, Susumu ^a   Sakaki, Shujiro ^c   Yamada, Satoshi ^c   Matsumoto, Ken ^d   Mizuguchi, Masahiro ^d   Yue, Fengming ^a   Tanaka, Yoshiki ^a  

^a SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b BOURBON Corporation   (Japan)

^c NOF Corporation   (Japan)

^d Nissui Pharmaceutical Co Ltd   (Japan)

Author keywords

EB formation; Hepatocyte differentiation; hES cells; Lanford medium; Microscopy; Suspension culture

Indexed keywords

2 METHACRYLOYLOXYETHYLPHOSPHORYLCHOLINE; ALBUMIN; BONE MORPHOGENETIC PROTEIN 4; CYTOKINE; GROWTH FACTOR; INDOCYANINE GREEN; PRIMER DNA;

ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYONIC STEM CELL; EXTRACELLULAR MATRIX; GERM LAYER; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; INTRAHEPATIC BILE DUCT; LANFORD MEDIUM; LIVER CELL; MARKER GENE; MATURATION; MESENCHYME; MESODERM; MORPHOLOGY; REACTION ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROUGH ENDOPLASMIC RETICULUM; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; CELL LINE; CYTOLOGY; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CELL LINE; DNA PRIMERS; EMBRYONIC STEM CELLS; HEPATOCYTES; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 70350749548     PISSN: None     EISSN: 1537744X     Source Type: Journal    
DOI: 10.1100/tsw.2009.97     Document Type: Article

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