Salivary sex hormone measurement in a national, population-based study of older adults

Journals of Gerontology - Series B Psychological Sciences and Social Sciences

Volumn 64, Issue SUPPL.1, 2009, Pages

  Gavrilova, Natalia ^a   Lindau, Stacy Tessler ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Aging; DHEA; Estradiol; Estrogen; Older; Progesterone; Saliva collection; Salivary; Sex hormone; Testosterone; Validity

Indexed keywords

ANDROGEN; ESTRADIOL; TESTOSTERONE;

AGED; AGING; ARTICLE; BLOOD; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESTROGEN THERAPY; FEMALE; HEALTH STATUS; HEALTH SURVEY; HORMONE SUBSTITUTION; HUMAN; MALE; MIDDLE AGED; OBESITY; OVARIECTOMY; PHYSIOLOGY; PSYCHOLOGICAL ASPECT; REFERENCE VALUE; SALIVA; SEX DIFFERENCE; SEXUAL BEHAVIOR; SOCIAL BEHAVIOR; STATISTICAL ANALYSIS; UNITED STATES;

AGED; AGED, 80 AND OVER; AGING; ANDROGENS; DATA INTERPRETATION, STATISTICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESTRADIOL; ESTROGEN REPLACEMENT THERAPY; FEMALE; HEALTH STATUS; HEALTH SURVEYS; HORMONE REPLACEMENT THERAPY; HUMANS; MALE; MIDDLE AGED; OBESITY; OVARIECTOMY; REFERENCE VALUES; SALIVA; SEX FACTORS; SEXUAL BEHAVIOR; SOCIAL BEHAVIOR; TESTOSTERONE; UNITED STATES;

EID: 70350721767     PISSN: 10795014     EISSN: None     Source Type: Journal    
DOI: 10.1093/geronb/gbn028     Document Type: Article

References (110)

1. Adashi, E. Y. (1994). The climacteric ovary as a functional gonadotropin- driven androgen-producing gland. Fertility and Sterility, 62, 20-27.
2. Ahn, R. S., Lee, Y. J., Choi, J. Y., Kwon, H. B., & Chun, S. I. (2007). Salivary cortisol and DHEA levels in the Korean population: Age-related differences, diurnal rhythm, and correlations with serum levels. Yonsei Medical Journal, 48, 379-388.
3. Akhmedkhanov, A., Zeleniuch-Jacquotte, A., & Toniolo, P. (2001). Role of exogenous and endogenous hormones in endometrial cancer - Review of the evidence and research perspectives. In C. Bulletti, D. de Ziegler, S. Guller & M. LevitzHuman (Eds.) Fertility and Reproduction: the Oocyte, the Embryo, and the Uterus, Annals of the New York Academy of Sciences, 943, 296-315.
4. Alexandersen, P., Haarbo, J., & Christiansen, C. (1996). The relationship of natural androgens to coronary heart disease in males: A review. Atherosclerosis, 125, 1-13.
5. Allan, C. A., Strauss, B. J., Burger, H. G., Forbes, E. A., & McLachlan, R. I. (2006). The association between obesity and the diagnosis of androgen defi ciency in symptomatic ageing men. Medical Journal of Australia, 185, 424-427.
6. Andersen, M. L., & Tufi k, S. (2006). Does male sexual behavior require progesterone? Brain Research. Brain Research Reviews, 51, 136-143.
7. Bachmann, G., & Leiblum, S. (2004). The impact of hormones on menopausal sexuality: A literature review. Menopause, 11, 120-130.
8. Barrett-Connor, E., Goodman-Gruen, D., & Patay, B. (1999). Endogenous sex hormones and cognitive function in older men. Journal of Clinical Endocrinology and Metabolism, 84, 3681-3685. (Pubitemid 30645225)
9. Bell, F. C., & Miller, M. L. (2002). Life tables for the United States Social Security area 1900-2100. Baltimore : Social Security Administration, Offi ce of Chief Actuary.
10. Binder, D. (1983). On the variances of asymptotically normal estimators from complex surveys. Statistical Review, 51, 279-292.
11. Brown, G. L., McGarvey, E. L., Shirtcliff, E. A., Keller, A., Granger, D. A., & Flavin, K. (2008). Salivary cortisol, dehydroepiandrosterone, and testosterone interrelationships in healthy young males: A pilot study with implications for studies of aggressive behavior. Psychiatry Research, 159, 67-76.
12. Cappola, A. R., Ratcliffe, S. J., Bhasin, S., Blackman, M. R., Cauley, J., Robbins, J., Zmuda, J. M., Harris, T., & Fried, L. P. (2007). Determinants of serum total and free testosterone levels in women over the age of 65 years. Journal of Clinical Endocrinology and Metabolism, 92 (2), 509-516.
13. Carlson, L. E., & Sherwin, B. B. (2000). Higher levels of plasma estradiol and testosterone in healthy elderly men compared with age-matched women may protect aspects of explicit memory. Menopause, 7, 168 - 177.
14. Carlson, M. C., Zandi, P. P., Plassman, B. L., Tschanz, J. T., Welsh-Bohmer, K. A., Steffens, D. C., Bastian, L. A., Mehta, K. M., & Breitner, J. C. (2001). Hormone replacement therapy and reduced cognitive decline in older women: The Cache County Study. Neurology , 57 (12), 2210-2216.
15. Celec, P., Ostatnikova, D., Hodosy, J., Skoknova, M., Putz, Z., & Kudela, M. (2006). Infradian rhythmic variations of salivary estradiol and progesterone in healthy men. Biological Rhythm Research, 37, 37-44.
16. Chlebowski, R. T., Hendrix, S. L., Langer, R. D., Stefanick, M. L., Gass, M., Lane, D., Rodabough, R. J., Gilligan, M. A., Cyr, M. G., Thomson, C. A. et al. (2003). Infl uence of estrogen plus progestin on breast cancer and mammography in healthy postmenopausal women: The Women's Health Initiative randomized trial. Journal of the American Medical Association, 289 (24), 3243-3253. (Pubitemid 37430077)
17. Choi, B. G., & McLaughlin, M. A. (2007). Why men's hearts break: Cardiovascular effects of sex steroids. Endocrinology and Metabolism Clinics of North America, 36, 365-377.
18. Christeff, N., Lortholary, O., Casassus, P., Thobie, N., Veyssier, P., Torri, O., Guillevin, L., & Nunez, E. A. (1996). Relationship between sex steroid hormone levels and CD4 lymphocytes in HIV infected men. Experimental and Clinical Endocrinology & Diabetes, 104, 130-136.
19. Clemons, M., & Goss, P. (2001). Estrogen and the risk of breast cancer. New England Journal of Medicine, 344, 276-285.
20. Davis, S., & Tran, J. (2001). What are " normal" testosterone levels for women? Journal of Clinical Endocrinology and Metabolism, 86, 1842-1844.
21. Dennerstein, L., Alexander, J. L., & Kotz, K. (2003). The menopause and sexual functioning: A review of the population-based studies. Annual Review of Sex Research, 14, 64-82.
22. Dennerstein, L., Gotts, G., Brown, J. B., Morse, C. A., Farley, T. M., & Pinol, A. (1994). The relationship between the menstrual cycle and female sexual interest in women with PMS complaints and volunteers . Psychoneuroendocrinology, 19, 293-304.
23. Dennerstein, L., Smith, A. M., Morse, C. A., & Burger, H. G. (1994). Sexuality and the menopause. Journal of Psychosomatic Obstetrics and Gynaecology, 15, 59-66.
24. Derby, C. A., Zilber, S., Brambilla, D., Morales, K. H., & McKinlay, J. B. (2006). Body mass index, waist circumference and waist to hip ratio and change in sex steroid hormones: The Massachusetts Male Ageing Study. Clinical Endocrinology, 65, 125-131.
25. Dorgan, J. F., Longcope, C., Franz, C., Stanczyk, F. Z., Chang, L. C., Stephenson, H. E., Falk, R. T., Kahle, L., Miller, R., Tangrea, J. A. et al. (2002). Endogenous sex hormones and breast cancer in postmenopausal women: Reanalysis of nine prospective studies. Journal of the National Cancer Institute, 94, 606-616.
26. Drum, M. L., Shiovitz-Ezra, S., Gaumer, E., & Lindau, S. T. (2009). Assessment of health-related behaviors and health care utilization in the National Social Life, Health, and Aging Project (NSHAP). Journals of Gerontology: Social Sciences.
27. Ellison, P. T., Bribiescas, R. G., Bentley, G. R., Campbell, B. C., Lipson, S. F., Panter-Brick, C., & Hill, K. (2002). Population variation In age-related decline in male salivary testosterone. Human Reproduction , 17, 3251-3253.
28. Emaus, A., Veierod, M. B., Furberg, A. S., Espetvedt, S., Friedenreich, C., Ellison, P. T., et al. (2008). Physical activity, heart rate, metabolic profi le, and estradiol in premenopausal women. Medicine and Science in Sports and Exercise, 40, 1022-1030.
29. Feldman, H. A., Longcope, C., Derby, C. A., Johannes, C. B., Araujo, A. B., Coviello, A. D., Bremner, W. J., & McKinlay, J. B. (2002). Age trends in the level of serum testosterone and other hormones in middle-aged men: Longitudinal results from the Massachusetts Male Aging Study. Journal of Clinical Endocrinology and Metabolism, 87 (2), 589-598.
30. Flegr, J., Lindova, J., & Kodym, P. (2008). Sex-dependent toxoplasmosisassociated differences in testosterone concentration in humans. Parasitology, 135, 427-431.
31. Geerlings, M. I., Launer, L. J., de Jong, F. H., Ruitenberg, A., Stijnen, T., van Swieten, J. C., Hofman, A., Witteman, J. C. M., Pols, H. A. P., & Breteler, M. M. B. (2003). Endogenous estradiol and risk of dementia in women and men: The Rotterdam study. Annals of Neurology, 53 (5), 607-615.
32. Giagulli, V. A., Kaufman, J. M., & Vermeulen, A. (1994). Pathogenesis of the decreased androgen levels in obese men. Journal of Clinical Endocrinology and Metabolism, 79, 997-1000.
33. Granger, D. A., Kivlighan, K. T., Fortunato, C., Harmon, A. G., Hibel, L. C., Schwartz, E. B., & Whembolua, G.-L. (2007). Integration of salivary biomarkers into developmental and behaviorally-oriented research: Problems and solutions for collecting specimens. Physiology and Behavior, 92, 583-590.
34. Granger, D. A., Schwartz, E. B., Booth, A., Curran, M., & Zakaria, D. (1999). Assessing dehydroepiandrosterone in saliva: A simple radioimmunoassay for use in studies of children, adolescents and adults. Psychoneuroendocrinology, 24, 567-579.
35. Granger, D. A., Shirtcliff, E. A., Booth, A., Kivlighan, K. T., & Schwartz, E. B. (2004). The " trouble " with salivary testosterone. Psychoneuroendocrinology , 29, 1229-1240.
36. Greenblatt, R. B., Oettinger, M., & Bohler, C. S. S. (1976). Estrogenandrogen levels in aging men and women: Therapeutic considerations . Journal of the American Geriatrics Society, 24, 173-178.
37. Greenspan, F. S., & Gardner, D. G. (2001). Basic & clinical endocrinology (6th ed.). New York : Lange Medical Books/McGraw-Hill.
38. Grinspoon, S., Corcoran, C., Stanley, T., Baaj, A., Basgoz, N., & Klibanski , A. (2000). Effects of hypogonadism and testosterone administration on depression indices in HIV-infected men. Journal of Clinical Endocrinology and Metabolism, 85, 60-65.
39. Guyton, A. C. (1991). Textbook of medical physiology (8th ed.). Philadelphia : Saunders.
40. Hackbert, L., & Heiman, J. R. (2002). Acute dehydroepiandrosterone (DHEA) effects on sexual arousal in postmenopausal women. Journal of Womens Health Gender Based Medicine, 11, 155-162.
41. Hall, J. M., Couse, J. F., & Korach, K. S. (2001). The multifaceted mechanisms of estradiol and estrogen receptor signaling. Journal of Biological Chemistry, 276, 36869-36872.
42. Hankinson, S. E., & Eliassen, A. H. (2007). Endogenous estrogen, testosterone and progesterone levels in relation to breast cancer risk. Journal of Steroid Biochemistry and Molecular Biology, 106, 24-30.
43. Harris, T. O., Borsanyi, S., Messari, S., Stanford, K., Cleary, S. E., Shiers, H. M., Brown, G. W., & Herbert, J. (2000). Morning cortisol as a risk factor for subsequent major depressive disorder in adult women. British Journal of Psychiatry, 177, 505-510.
44. Hendrix, S. L. (2005). Bilateral oophorectomy and premature menopause. American Journal of Medicine, 118 (Suppl. 12B), 131-135.
45. Hodgson, N., Freedman, V. A., Granger, D. A., & Erno, A. (2004). Biobehavioral correlates of relocation in the frail elderly: Salivary cortisol, affect, and cognitive function. Journal of the American Geriatrics Society, 52 (11), 1856-1862.
46. Hosmer, D. W., & Lemeshow, S. (1989). Applied logistic regression. New York : John Wiley & Sons.
47. Jankowska, E. A., Biel, B., Majda, J., Szklarska, A., Lopuszanska, M., Medras, M., Anker, S. D., Banasiak, W., Poole-Wilson, P. A., & Ponikowski, P. (2006). Anabolic defi ciency in men with chronic heart failure: Prevalence and detrimental impact on survival. Circulation, 114 (17), 1829-1837.
48. Jasienska, G., & Jasienski, M. (2008). Interpopulation, interindividual, intercycle, and intracycle natural variation in progesterone levels: A quantitative assessment and implications for population studies. American Journal of Human Biology, 20, 35-42.
49. Jassal, S. K., Barrett-Connor, E., & Edelstein, S. L. (1995). Low bioavailable testosterone levels predict future height loss in postmenopausal women. Journal of Bone and Mineral Research, 10, 650-654.
50. Johnson, M. D., Bebb, R. A., & Sirrs, S. M. (2002). Uses of DHEA in aging and other disease states. Ageing Research Reviews, 1, 29-41.
51. Kaufman, E., & Lamster, I. B. (2002). The diagnostic applications of saliva - a review. Critical Reviews in Oral Biology and Medicine, 13, 197-212.
52. Kaufman, J. M., & Vermeulen, A. (2005). The decline of androgen levels in elderly men and its clinical and therapeutic implications. Endocrine Reviews, 26, 833-876.
53. Keshavarz, H., Hillis, S. D., Kieke, B. A., & Marchbanks, P. A. (2002). Hysterectomy surveillance - United States, 1994 - 1999. Morbidity and Mortality Weekly Report. CDC Surveillance Summaries, 51 (SS05), 1-8.
54. Khaw, K. T., Dowsett, M., Folkerd, E., Bingham, S., Wareham, N., Luben, R., Welch, A., & Day, N. (2007). Endogenous testosterone and mortality due to all causes, cardiovascular disease, and cancer in men: European prospective investigation into cancer in Norfolk (EPIC-Norfolk) prospective population study. Circulation, 116, 2694-2701.
55. Krause, W., Mueller, U., & Mazur, A. (2002). Measurement of steroid levels in saliva in a population-based survey of lifestyle, medical conditions, marriage, sex life and hormone status in aging men: A feasibility study. The Aging Male, 5, 203-215.
56. Kuchuk, N., van Schoor, N., Pluijm, S., Smit, J., de Ronde, W., & Lips, P. (2007). The association of sex hormone levels with quantitative ultrasound, bone mineral density, bone turnover and osteoporotic fractures in older men and women. Clinical Endocrinology, 67, 295-303.
57. Labrie, F., Belanger, A., Cusan, L., Gomez, J. L., & Candas, B. (1997). Marked decline in serum concentrations of adrenal C19 sex steroid precursors and conjugated androgen metabolites during aging. Journal of Clinical Endocrinology and Metabolism, 82, 2396-2402.
58. Laughlin, G. A., Barrett-Connor, E., Kritz-Silverstein, D., & von Muhlen, D. (2000). Hysterectomy, oophorectomy, and endogenous sex hormone levels in older women: The Rancho Bernardo study. Journal of Clinical Endocrinology and Metabolism, 85, 645-651. (Pubitemid 32273648)
59. Lindau, S. T., & Gavrilova, N. S. (2008). Understanding overall and sexual health in older women using saliva sex hormone measurements, Population Association of America April 2008 Annual Meeting. Final Program and Abstracts (p. 226). Silver Spring, MD: Population Association of America.
60. Lindau, S. T., Hoffmann, J. N., Lundeen, K., Jaszczak, A., McClintock, M. K., & Jordan, J. A. (2009). Vaginal self-swab specimen collection In a home-based survey of older women: Methods and applications. Journals of Gerontology: Social Sciences, this issue.
61. Lindau, S. T., & McDade, T. W. (2007). Minimally invasive and innovative methods for biomeasure collection in population-based research. In Maxine Weinstein, James W. Vaupel, and Kenneth W. Wachter (Eds.), Biosocial Surveys (pp. 251 - 277). Washington, DC: The National Academies Press.
62. Lindau, S. T., Schumm, P., Laumann, E. O., Levinson, W., O'Muircheartaigh, C., & Waite, L. (2007). A national study of sexuality and health among older adults in the U.S. New England Journal of Medicine , 357, 22-34.
63. Lobo, R. A. (2001). Androgens in postmenopausal women: Production, possible role, and replacement options. Obstetrical & Gynecological Survey, 56, 361-376.
64. Lu, Y., Bentley, G. R., Gann, P. H., Hodges, K. R., & Chatterton, R. T. (1999). Salivary estradiol and progesterone levels in conception and nonconception cycles in women: Evaluation of a new assay for salivary estradiol. Fertility and Sterility, 71, 863-868.
65. Lukanova, A., Lundin, E., Zeleniuch-Jacquotte, A., Muti, P., Mure, A., Rinaldi, S., Dossus, L., Micheli, A., Arslan, A., Lenner, P. et al. (2004). Body mass index, circulating levels of sex-steroid hormones, IGF-I and IGF-binding protein-3: A cross-sectional study In healthy women. European Journal of Endocrinology, 150, 161-171.
66. Maggio, M., Lauretani, F., Ceda, G. P., Bandinelli, S., Ling, S. M., Metter, E. J., Artoni, A., Carassale, L., Cazzato, A., Ceresini, G. et al. (2007). Relationship between low levels of anabolic hormones and 6-year mortality in older men The Aging in the Chianti Area (InCHIANTI) study. Archives of Internal Medicine, 167 (20), 2249-2254.
67. Maki, P. M., & Resnick, S. M. (2000). Longitudinal effects of estrogen replacement therapy on PET cerebral blood fl ow and cognition. Neurobiology of Aging, 21, 373-383.
68. Manly, J. J., Merchant, C. A., Jacobs, D. M., Small, S. A., Bell, K., Ferin, M., & Mayeux, R. (2000). Endogenous estrogen levels and Alzheimer's disease among postmenopausal women. Neurology, 54, 833-837.
69. Manson, J. E., Hsia, J., Johnson, K. C., Rossouw, J. E., Assaf, A. R., Lasser, N. L., Trevisan, M., Black, H. R., Heckbert, S. R., Detrano, R. et al. (2003). Estrogen plus progestin and the risk of coronary heart disease. New England Journal of Medicine, 349, 523-534.
70. Masi, A. T. (1995). Sex-hormones and rheumatoid-arthritis: Cause or effect relationships in a complex pathophysiology. Clinical and Experimental Rheumatology, 13, 227-240.
71. McQuillan, G., Pan, Q., & Porter, K. (2006). Consent for genetic research in a general population: An update on the National Health and Nutrition Examination Survey experience. Genetics in Medicine, 8, 354-360.
72. Mendoza, K., Curran, M. J., & Lindau, S. T. (2007a). Salivary DHEA measurement in Wave I of the National Social Life, Health & Aging Project (NSHAP). Chicago : NORC and the University of Chicago.
73. Mendoza, K., Curran, M. J., & Lindau, S. T. (2007b). Salivary estradiol measurement in Wave I of the National Social Life, Health & Aging Project (NSHAP). Chicago : NORC and the University of Chicago.
74. Mendoza, K., Curran, M. J., & Lindau, S. T. (2007c). Salivary testosterone measurement in Wave I of the National Social Life, Health & Aging Project (NSHAP). Chicago : NORC and the University of Chicago.
75. Meston, C. M., & Frohlich, P. F. (2000). The neurobiology of sexual function . Archives of General Psychiatry, 57, 1012-1030.
76. Morsink, L. F. J., Vogelzangs, N., Nicklas, B. J., Beekman, A. T. F., Satterfield, S., Rubin, S. M., Yaffe, K., Simonsick, E., Newman, A. B., Kritchevsky, S. B. et al. (2007). Associations between sex steroid hormone levels and depressive symptoms in elderly men and women: Results from the Health ABC study. Psychoneuroendocrinology , 32 (8-10), 874-883.
77. Muller, M., van der Schouw, Y. T., Thijssen, J. H. H., & Grobbee, D. E. (2003). Endogenous sex hormones and cardiovascular disease in men. Journal of Clinical Endocrinology and Metabolism, 88, 5076-5086. (Pubitemid 37452697)
78. Nallanathan, B., Mendoza, K. M., Curran, M. J., & Lindau, S. T. (2007). Salivary progesterone measurement in wave I of the National Social Life, Health & Aging Project (NSHAP). Chicago : NORC and the University of Chicago.
79. Newton, K. M., Buist, D. S. M., Yu, O., Hartsfi eld, C. L., Andrade, S. E., Wei, F., Connelly, M. T., & Chan, K. A. (2008). Hormone therapy initiation after the women's health initiative. Menopause: The Journal of the North American Menopause Society, 15 (3), 487-493.
80. Nichols, H. B., Trentham-Dietz, A., Hampton, J. M., Titus-Ernstoff, L., Egan, K. M., Willett, W. C., & Newcomb, P. A. (2006). From menarche to menopause: Trends among US women born from 1912 to 1969. American Journal of Epidemiology, 164 (10), 1003-1011.
81. Nunez-De La Mora, A., Bentley, G. R., Choudhury, O. A., Napolitano, D. A., & Chatterton, R. T. (2008). The impact of developmental conditions on adult salivary estradiol levels: Why this differs from progesterone? American Journal of Human Biology, 20, 2-14.
82. O'Muircheartaigh, C., Eckman, S., & Smith, S. (2009). Statistical design and estimation for the National Social Life, Health, and Aging Project (NSHAP). Journal of Gerontology: Social Sciences, this issue.
83. Panjari, M., & Davis, S. (2007). DHEA therapy for women: Effect on sexual function and wellbeing. Human Reproduction Update, 13, 239-248.
84. Rantala, M. J., Enksson, C. J. P., Vainikka, A., & Kortet, R. (2006). Male steroid hormones and female preference for male body odor. Evolution and Human Behavior, 27, 259-269.
85. Ravaglia, G., Forti, P., Maioli, F., Bastagli, L., Montesi, F., Pisacane, N., Chiappelli, M., Licastro, F., & Patterson, C. (2007). Endogenous sex hormones as risk factors for dementia in elderly men and women. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 62 (9), 1035-1041.
86. Ravaglia, G., Forti, P., Maioli, F., Boschi, F., Bernardi, M., Pratelli, L., Pizzoferrato, A., & Gasbarrini, G. (1996). The relationship of dehydroepiandrosterone sulfate (DHEAS) to endocrine-metabolic parameters and functional status in the oldest-old. Results from an Italian study on healthy free-living over-ninety-year-olds. Journal of Clinical Endocrinology and Metabolism, 81 (3), 1173-1178.
87. Regelson, W., Loria, R., & Kalimi, M. (1994). Dehydroepiandrosterone (Dhea) - the mother steroid. 1. Immunological action. Aging Clock, 719, 553-563.
88. Rehman, H. U., & Masson, E. A. (2005). Neuroendocrinology of female aging. Gender Medicine, 2, 41-56.
89. Rehman, K. S., & Carr, B. R. (2004). Sex differences in adrenal androgens. Seminars in Reproductive Medicine, 22, 349-360.
90. Rossouw, J. E., Anderson, G. L., Prentice, R. L., LaCroix, A. Z., Kooperberg, C., Stefanick, M. L., Jackson, R. D., Beresford, S. A. A., Howard, B. V., Johnson, K. et al. (2002). Risks and benefi ts of estrogen plus progestin in healthy postmenopausal women: Principal results from the women's health initiative randomized controlled trial. Journal of the American Medical Association, 288 (3), 321-333.
91. Salimetrics. (2006). High sensitivity salivary estradiol enzyme immunoassay kit. State College, PA : Salimetrics LLC.
92. Schilling, C., Gallicchio, L., Miller, S. R., Langenberg, P., Zacur, H., & Flaws, J. A. (2007). Relation of body mass and sex steroid hormone levels to hot fl ushes in a sample of mid-life women. Climacteric, 10, 27-37.
93. Schultheiss, O. C., Dargel, A., & Rohde, W. (2003). Implicit motives and gonadal steroid hormones: Effects of menstrual cycle phase, oral contraceptive use, and relationship status. Hormones and Behaviour, 43, 293-301.
94. Shirtcliff, E. A., Granger, D. A., & Likos, A. (2002). Gender differences In the validity of testosterone measured in saliva by immunoassay. Hormones and Behaviour, 42, 62-69.
95. Shores, M. M., Matsumoto, A. M., Sloan, K. L., & Kivlahan, D. R. (2006). Low serum testosterone and mortality in male veterans. Archives of Internal Medicine, 166, 1660-1665.
96. Spark, R. F. (2002). Dehydroepiandrosterone: A springboard hormone for female sexuality. Fertility and Sterility, 77 (Suppl. 4), S19-S25.
97. Speroff, L., Glass, R. H., & Kase, N. G. (1999). Clinical gynecologic endocrinology and infertility (6th ed.). Philadelphia : Lippincott Williams & Wilkins.
98. StataCorp. (2005). Stata statistical software: Release 9. College Station, TX : StataCorp LP.
99. Tenover, J. L. (1997). Testosterone and the aging male. Journal of Andrology , 18, 103-106.
100. Tivis, L. J., Richardson, M. D., Peddi, E., & Arjmandi, B. (2005). Saliva versus serum estradiol: Implications for research studies using postmenopausal women. Progess in Neuro-Psychopharmacology & Biological Psychiatry, 29, 727-732.
101. U.S. Department of Health and Human Services, National Institutes of Health(2000). Clinical guidelines on the identifi cation, evaluation, and treatment of overweight and obesity in adults: The evidence report [document 98 - 4083]. Bethesda, MD : NHLBI.
102. van Niekerk, J. K., Huppert, F. A., & Herbert, J. (2001). Salivary cortisol and DHEA: Association with measures of cognition and well-being in normal older men, and effects of three months of DHEA supplementation . Psychoneuroendocrinology, 26, 591-612.
103. Villareal, D. T., & Holloszy, J. O. (2004). Effect of DHEA on abdominal fat and insulin action in elderly women and men: A randomized controlled trial. Journal of the American Medical Association, 292, 2243-2248.
104. Whembolua, G.-L., Granger, D. A., Kivlighan, K. T., Marguin, J. A., & Singer, S. (2006). Bacteria in the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva. Hormones and Behavior, 49, 478-483.
105. Williams, R. H., & Wilson, J. D. (1998). Williams textbook of endocrinology (9th ed.). Philadelphia : Saunders.
106. Williams, S. R., & McDade, T. W. (2009). The use of dried blood spot sampling in the National Social Life, Health, and Aging Project. Journal of Gerontology: Social Sciences, this issue.
107. Wirth, M. M., Meier, E. A., Fredrickson, B. L., & Schultheiss, O. C. (2007). Relationship between salivary cortisol and progesterone levels in humans . Biological Psychology, 74, 104-107.
108. Wirth, M. M., & Schultheiss, O. C. (2006). Effects of affi liation arousal (hope of closeness) and affi liation stress (fear of rejection) on progesterone and cortisol. Hormones and Behavior, 50, 786-795.
109. Worthman, C. M., Stallings, J. F., & Hofman, L. F. (1990). Sensitive salivary estradiol assay for monitoring ovarian function. Clinical Chemistry , 36, 1769-1773.
110. Wysowski, D. K., Golden, L., & Burke, L. (1995). Use of menopausal estrogens and medroxyprogesterone in the United States, 1982 - 1992. Obstetrics & Gynecology, 85, 6-10.