Cell-free protein synthesis using multiply-primed rolling circle amplification products

BioTechniques

Volumn 47, Issue 1, 2009, Pages 637-639

  Kumar, Gyanendra ^a   Chernaya, Galina ^a  

^a GE Healthcare   (United States)

Author keywords

29 DNA polymerase; In vitro transcription and translation; Multiply primed rolling circle amplification

Indexed keywords

DNA POLYMERASE; CIRCULAR DNA; DNA DIRECTED DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; HISTIDINE; VIRUS DNA;

ARTICLE; DNA STRAND; DNA STRUCTURE; DNA TEMPLATE; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; IN VITRO STUDY; PLASMID; PROTEIN SYNTHESIS; BACILLUS PHAGE; CELL FREE SYSTEM; CHEMISTRY; DNA REPLICATION; ENZYMOLOGY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; PROMOTER REGION; TEMPERATURE; TIME;

BACILLUS PHAGES; CELL-FREE SYSTEM; DNA REPLICATION; DNA, CIRCULAR; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; GREEN FLUORESCENT PROTEINS; HISTIDINE; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PLASMIDS; PROMOTER REGIONS, GENETIC; PROTEIN BIOSYNTHESIS; TEMPERATURE; TEMPLATES, GENETIC; TIME FACTORS;

EID: 70350720040     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113171     Document Type: Article

References (24)

1. Katzen, F., G. Chang, and W. Kudlicki. 2005. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23:150-153.
2. Woodrow, K.A., I.O. Airen, and J.A. Swartz. 2006. Rapid expression of functional genomic libraries. J. Proteome Res. 5:3288-3300.
3. Goerke, A.R. and J.R. Swartz. 2008. Development of cell-free protein synthesis platforms for disulfide bonded proteins. Biotechnol. Bioeng. 99:351-367.
4. Yokoyama, S. 2003. Protein expression systems for structural genomics and proteomics. Curr. Opin. Chem. Biol. 7:39-43.
5. Sawasaki, T., R. Morishita, M.D. Gouda, and Y. Endo. 2007. Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system. Methods Mol. Biol. 375:95-106.
6. Endo, Y. and T. Sawasaki. 2003. High throughput, genome-scale protein production method based on the wheat germ cell-free expression system. Biotechnol. Adv. 21:695-713.
7. Spirin, A.S. and J.R. Swartz. 2007. Cell-free Protein Synthesis: Methods and Protocols. John Wiley and Sons, NY.
8. Calhoun, K.A. and J.A. Swartz. 2006. Total amino acid stabilization during cell-free protein synthesis reactions. J. Biotechnol. 123:193-203.
9. Calhoun, K.A. and J.A. Swartz. 2005. An economical method for cell-free protein synthesis using glucose and nucleoside monophosphates. Biotechnol. Prog. 21:1146-1153.
10. Calhoun, K.A. and J.A. Swartz. 2005. Energizing cell-free protein synthesis with glucose metabolism. Biotechnol. Bioeng. 90:606-613.
11. Jewett, M.C. and J.R. Swartz. 2004. Substrate replishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm. Biotechnol. Bioeng. 87:465-472.
12. Kigawa, T. and S. Yokoyama. 1991. A continuous cell-free protein synthesis system for coupled transcription-translation. J. Biochem (Japan) 110:166-168.
13. Shimizu, Y., A. Inoue, Y. Tomari, T. Suzuki, T. Yokogawa, K. Nishikawa, and T. Ueda. 2001. Cell-free translation reconstituted with purified components. Nat. Biotechnol. 19:751-755.
14. Mei, Q., C.K. Fredrickson, A. Simon, R. Khnouf, and Z.H. Fan. 2007. Cell-free protein synthesis in microfluidic array devices. Biotechnol. Prog. 23:1305-1311.
15. Andreadis, J.D. and L.A. Chrisey. 2000. Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions. Nucleic Acids Res. 28:e5.
16. DiTursi, M.K.W., J. Cha, M.R. Newman, and J.S. Dordick. 2004. Simultaneous in vitro protein synthesis using solid-phase DNA templates. Biotechnol. Prog. 20:1705-1709.
17. Sawasaki, T., T. Ogasawara, R. Morishita, and Y. Endo. 2002. A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl. Acad. Sci. USA 99:14652-14657.
18. Bardóczy, V., V. Géczi, T. Sawasaki, Y. Endo, and T. Mészáros. 2008. A set of ligation-independent in vitro translation vectors for eukaryotic protein production. BMC Biotechnol. 8:32. (Pubitemid 351546709)
19. Dean, F.B., J.R. Nelson, T.L. Gisler, and R.L. Lasken. 2001. Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multi-primed rolling circle amplification. Genome Res. 11:1095-1099.
20. Rothe, A., R.N. Surjadi, and B.E. Power. 2006. Novel proteins in emulsions using in vitro compartmentalization. Trends Biotechnol. 24:587-592.
21. Kumar, G., G. Garnova, M. Reagin, and A. Vidali. 2008. Improved multiple displacement amplification with Phi29 DNA polymerase for genotyping of single human cells. BioTechniques 44:879-890.
22. Lustig, K.D., P.T. Stukenberg, T.J. McGary, R.W. King, V.L. Cyrus, and P.E. Mead. 1997. Small pool expression screening: identification of genes involved in cell cycle control, apoptosis, and early development. Methods Enzymol. 283:83-99.
23. Murthy, T.V.S., W. Wu, Q.Q. Qiu, Z. Shi, J. LaBaer, and L. Brizuela. 2004. Bacterial cell-free system for high-throughput protein expression and comparative analysis of Escherichia coli cell-free and whole cell expression systems. Protein Expr. Purif. 36:217-225.
24. Hutchison, C.A., III, H.O. Smith, C. Pfannkoch, and J.C. Venter. 2005. Cell-free cloning using Phi29 DNA polymerase. Proc. Natl. Acad. Sci. USA 102:17332-17336.