Fast, quantitative in situ hybridization of rare mRNAs using 14C-standards and phosphorus imaging

Journal of Neuroscience Methods

Volumn 185, Issue 1, 2009, Pages 56-61

  Cremer, Christian M ^a,b   Cremer, Markus ^a   Escobar, Jennifer Lopez ^a   Speckmann, Erwin J ^c   Zilles, Karl ^a,b  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Autoradiography; Dimethyl arsenic acid; Microscales; Neurotransmitter receptors; Phosphorus imaging; Quantitative in situ hybridization; Standardization

Indexed keywords

AMPA RECEPTOR; CACODYLIC ACID; CARBON 14; GLUTAMATE RECEPTOR 1; GLUTAMATE RECEPTOR 2; MESSENGER RNA; NEUROTRANSMITTER RECEPTOR; PHOSPHORUS 33;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; CONTROLLED STUDY; DOSIMETRY; GENE EXPRESSION; HIPPOCAMPUS; IMAGE ANALYSIS; IMAGING SYSTEM; IN SITU HYBRIDIZATION; ISOTOPE LABELING; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RADIOACTIVITY; RAT; RNA HYBRIDIZATION;

ANIMALS; CACODYLIC ACID; CARBON RADIOISOTOPES; HIPPOCAMPUS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION; MALE; NEUROCHEMISTRY; OLIGONUCLEOTIDES; PHOSPHORUS RADIOISOTOPES; RATS; RATS, WISTAR; RECEPTORS, AMPA; REFERENCE STANDARDS; RNA, MESSENGER; TIME FACTORS;

EID: 70350710010     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2009.09.010     Document Type: Article

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