A simple method for purification of genomic DNA from whole blood using Fe3O4/Au composite particles as a carrier

Journal of Medical Colleges of PLA

Volumn 24, Issue 4, 2009, Pages 239-243

  Ming, Zhao ^a   Xianqing, Zhang ^b   Sen, Wang ^a   Chao, Chen ^a   Yali, Cui ^a  

^a NORTHWEST UNIVERSITY   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Fe3O4 Au composite particles; Genomic DNA; Purification; Whole blood

Indexed keywords

GENOMIC DNA; GOLD NANOPARTICLE; MAGNETITE; NANOCOMPOSITE; SODIUM CHLORIDE;

AGAR GEL ELECTROPHORESIS; ARTICLE; BINDING AFFINITY; BLOOD SAMPLING; COMPOSITE MATERIAL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; GENOME SIZE; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION;

EID: 70350588652     PISSN: 10001948     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1000-1948(09)60044-7     Document Type: Article

References (13)

1. Hawkins T.L., O'Connor-Morin T., Roy A., et al. DNA purification and isolation using a solid-phase. Nucleic Acids Res 22 21 (1994) 4543-4544
2. Berensmeier S. Magnetic particles for the separation and purification of nucleic acids. Appl Microbiol Biotechnol 73 3 (2006) 495-504
3. 4) for isolation of genomic DNA from mammalian cells. Anal Biochem 356 2 (2006) 306-308
4. Nakagawa T., Tanaka T., Niwa D., et al. Fabrication of amino silane-coated microchip for DNA extraction from whole blood. J Biotech 116 2 (2005) 105-111
5. Park H.Y., Schadt M.J., Wang L., et al. Fabrication of magnetic coreShell Fe Oxide@Au nanoparticles for interfacial bioactivity and bio-separation. Langmuir 23 17 (2007) 9050-9056
6. Mosier-Boss P.A., and Lieberman S.H. Surface-enhanced raman spectroscopy substrate composed of chemically modified gold colloid particles Iimmobilized on magnetic microparticles. Anal Chem 77 4 (2005) 1031-1037
7. 4/Au core/shell super-paramagnetic microspheres. Sci China Ser B 44 4 (2001) 404-410 (In Chinese)
8. Cui Y.L., Zhang L.Y., Su Q., et al. Synthesis of GoldMag particles with assembled structure and their applications in immunoassay. Sci China Ser B 49 6 (2006) 534-540 (In Chinese)
9. Taylor J.I., Hurst C.D., Davies M.J., et al. Application of magnetite and silica-magnetite composites to the isolation of genomic DNA. J Chrom A 890 1 (2000) 159-166
10. Prodelalova J., Rittich B., Spanova A., et al. Isolation of genomic DNA using magnetic cobalt ferrite and silica particles. J Chrom A 1056 1-2 (2004) 43-48
11. Xie X., Nie X., Yu B., et al. Rapid enrichment of leucocytes and genomic DNA from blood based on bifunctional core-shell magnetic nanoparticles. JMMM 311 1 (2007) 416-420
12. Corach D., Filqueira Risso L., Marino M., et al. Routine Y-STR typing in forensic casework. Forensic Sci Int 118 2-3 (2001) 131-135
13. Kayser M., and Sajantila A. Mutations at Y-STR loci: implications for paternity testing and forensic analysis. Forensic Sci Int 118 2 (2001) 116-121