Development of a PCR assay for typing and subtyping of Brucella species

International Journal of Medical Microbiology

Volumn 299, Issue 8, 2009, Pages 563-573

  Huber, Birgit ^a   Scholz, Holger C ^b   Lucero, Nidia ^c   Busse, Hans Jürgen ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^b BUNDESWEHR INSTITUTE OF MICROBIOLOGY   (Germany)

^c Administracion Nacional de Laboratorios E Institutos de Salud Dr Carlos G Malbran   (Argentina)

Author keywords

Brucella; Detection; Genetic fingerprinting; Identification; Molecular diagnostics; Multiplex PCR

Indexed keywords

ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BIOTYPE; BRUCELLA; BRUCELLA ABORTUS; BRUCELLA CANIS; BRUCELLA SUIS; CONTROLLED STUDY; GENE DELETION; GENE INSERTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; OCHROBACTRUM; RANDOM AMPLIFIED POLYMORPHIC DNA;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; BRUCELLA; BRUCELLOSIS; DNA FINGERPRINTING; DNA PRIMERS; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 70350574665     PISSN: 14384221     EISSN: 16180607     Source Type: Journal    
DOI: 10.1016/j.ijmm.2009.05.002     Document Type: Article

References (35)

1. Alton G.G., Jones L.M., Angus R.D., and Verger J.M. Techniques for the Brucellosis Laboratory (1988), INRA, Paris pp. 13-61
2. Bricker B.J., and Halling S.M. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32 (1994) 2660-2666
3. Cloeckaert A., Grayon M., and Grepinet O. An IS711 element downstream of the bp26 gene is a specific marker of Brucella spp. isolated from marine mammals. Clin. Diagn. Lab. Immunol. 7 (2000) 835-839
4. Cloeckaert A., Grayon M., Grepinet O., and Bourmedine K.S. Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests. Microbes Infect. 5 (2003) 593-602
5. Corbel M.J. Brucellosis: an overview. Emerg. Infect. Dis. 3 (1997) 213-221
6. Corbel M.J., and Banai M. Genus I. Brucella Meyer and Shaw 1920, 173AL. In: Brenner D.J., Krieg N.R., Staley J.T., and Garrity G.M. (Eds). Bergey's Manual of Systematic Bacteriology Vol. 2 (2005), Springer, New York 370-386
7. Fekete A., Bantle J.A., Halling S.M., and Stich R.W. Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers. J. Bacteriol. 174 (1992) 7778-7783
8. Ferrao-Beck L., Cardoso R., Munoz P.M., de Miguel M.J., Albert D., Ferreira A.C., Marin C.M., Thiebaud M., Jacques I., Grayon M., Zygmunt M.S., Garin-Bastuji B., Blasco J.M., and Inacia Sa M. Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine. Vet. Microbiol. 115 (2006) 269-277
9. Foster G., Osterman B.S., Godfroid J., Jacques I., and Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 57 (2007) 2688-2693
10. Foster J.T., Okinaka R.T., Svensson R., Shaw K., De B.K., Robison R.A., Probert W.S., Kenefic L.J., Brown W.D., and Keim P. Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades. J. Clin. Microbiol. 46 (2008) 296-301
11. Fretin D., Whatmore A.M., Al Dahouk S., Neubauer H., Garin-Bastuji B., Albert D., Van Hessche M., Menart M., Godfroid J., Walravens K., and Wattiau P. Brucella suis identification and biovar typing by real-time PCR. Vet. Microbiol. 131 (2008) 376-385
12. Gandara B., Merino A.L., Rogel M.A., and Martinez-Romero E. Limited genetic diversity of Brucella spp. J. Clin. Microbiol. 39 (2001) 235-240
13. Garcia-Yoldi D., Marin C.M., de Miguel M.J., Munoz P.M., Vizmanos J.L., and Lopez-Goni I. Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin. Chem. 52 (2006) 779-781
14. Gee J.E., De B.K., Levett P.N., Whitney A.M., Novak R.T., and Popovic T. Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. J. Clin. Microbiol. 42 (2004) 3649-3654
15. Groussaud P., Shankster S.J., Koylass M.S., and Whatmore A.M. Molecular typing divides marine mammal strains of Brucella into at least three groups with distinct host preferences. J. Med. Microbiol. 56 (2007) 1512-1518
16. Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., and Olsen S.C. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J. Bacteriol. 187 (2005) 2715-2726
17. Hinic V., Brodard I., Thomann A., Cvetnic Z., Frey J., and Abril C. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J. Microbiol. Meth. 75 (2008) 375-378
18. Jacques I., Grayon M., and Verger J.-M. Oxidative metabolic profiles of Brucella strains isolated from marine mammals: contribution to their species classification. FEMS Microbiol. Lett. 270 (2007) 245-249
19. Lane D.J. 16S/23S sequencing. In: Stackebrandt E., and Goodfellow M. (Eds). Nucleic Acid Techniques in Bacterial Systematics (1991), Wiley, Chichester 115-175
20. Ocampo-Sosa A.A., Agüero-Balbin J., and Garcia-Lobo J.M. Development of a new PCR assay to identify Brucella abortus biovars 5, 6 and 9 and the new subgroup 3b of biovar 3. Vet. Microbiol. 110 (2005) 41-51
21. Osterman B., and Moriyon I. International Committee on Systematics of Prokaryotes. Subcommittee on the taxonomy of Brucella. Minutes of the meeting, 17 September 2003, Pamplona, Spain. Int. J. Syst. Evol. Microbiol. 56 (2006) 1173-1175
22. Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L., Brinkac L.M., Beanan M.J., Daugherty S.C., Deboy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., and Fraser C.M. The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. USA 99 (2002) 13148-13153
23. Rajashekara G., Glasner J.D., Glover D.A., and Splitter G.A. Comparative whole-genome hybridization reveals genomic islands in Brucella species. J. Bacteriol. 186 (2004) 5040-5051
24. Ratushna V.G., Sturgill D.M., Ramamoorthy S., Reichow S.A., He Y., Lathigra R., Sriranganathan N., Halling S.M., Boyle S.M., and Gibas C.J. Molecular targets for rapid identification of Brucella spp. BMC Microbiol. 6 (2006) 13
25. Richards G.M. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal. Biochem. 57 (1974) 369-376
26. Scholz H.C., Pfeffer M., Witte A., Neubauer H., Al Dahouk S., Wernery U., and Tomaso H. Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene. J. Med. Microbiol. 57 (2008) 64-71
27. Scholz H.C., Hubalek Z., Sedlácek I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kämpfer P., Neubauer H., Cloeckaert A., Maquart M., Zygmunt M.S., Whatmore A.M., Falsen E., Bahn P., Göllner C., Pfeffer M., Huber B., Busse H.-J., and Nöckler K. Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol. 58 (2008) 375-382
28. Scholz H.C., Hubalek Z., Nesvadbova J., Tomaso H., Vergnaud G., Le Fleche P., Whatmore A.M., Dahouk S., Krüger M., Lodri C., and Pfeffer M. Isolation of Brucella microti from soil. Emerg. Infect. Dis. 14 (2008) 1316-1317
29. Scholz, H.C., Hofer, E. Vergnaud, G., Le Fleche, P., Whatmore, A.M., Al Dahouk, S., Pfeffer, M., Krüger, M., Cloeckaert, A., Tomaso, H., 2009. Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Vector-Borne Zoonotic Dis., in press (2008, Oct. 30; epub ahead of print), doi:10.1089/vbz.2008.0036.
30. Tcherneva E., Rijpens N., Jersek B., and Herman L.M.F. Differentiation of Brucella species by random amplified polymorphic DNA analysis. J. Appl. Microbiol. 88 (2000) 69-80
31. Velasco J., Romero C., Lopez-Goni I., Leiva J., Diaz R., and Moriyon I. Evaluation of the relatedness of Brucella ssp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella ssp. Int. J. Syst. Bacteriol. 48 (1998) 759-768
32. Verger J.-M., Grimont F., Grimont P.A.D., and Grayon M. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 35 (1985) 292-295
33. Whatmore A.M., Shankster S.J., Perrett L.L., Murphy T.J., Brew S.D., Thirlwall R.E., Cutler S.J., and MacMillan A.P. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J. Clin. Microbiol. 44 (2006) 1982-1993
34. Whatmore A.M., Perrett L.L., and MacMillan A.P. Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiol. 7 (2007) 34
35. Wieser M., and Busse H.-J. Rapid identification of Staphylococcus epidermidis. Int. J. Syst. Evol. Microbiol. 50 (2000) 1087-1093