Development and validation of a recombinant nucleocapsid protein-based ELISA for detection of the antibody to porcine reproductive and respiratory syndrome virus

Journal of Microbiology

Volumn 47, Issue 5, 2009, Pages 582-588

  Chu, Jia Qi ^a   Hu, Xu Min ^a   Kim, Myung Cheol ^a   Park, Chang Sik ^a   Jun, Moo Hyung ^a  

^a Chungnam National University   (South Korea)

Author keywords

Indirect ELISA; Nucleocapsid protein; PRRSV

Indexed keywords

DIAGNOSTIC AGENT; NUCLEOCAPSID PROTEIN; VIRUS ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL; ARTERIVIRUS; ARTICLE; BLOOD; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PREDICTION AND FORECASTING; SENSITIVITY AND SPECIFICITY; SWINE; VALIDATION STUDY; VIROLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, VIRAL; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; MOLECULAR SEQUENCE DATA; NUCLEOCAPSID PROTEINS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SWINE;

ARTERIVIRUS; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE; SUS;

EID: 70350547586     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: 10.1007/s12275-009-0033-x     Document Type: Article

References (29)

1. Albina, E., Y. Leforban, T. Baron, J.P. Plana Duran, and P. Vannier. 1992. An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus. Ann. Rech. Vet. 23, 167-176.
2. Cavanaugh, D. 1997. Nidovirales: A new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 142, 629-633.
3. Cho, H.J., D. Deregt, and H.S. Joo. 1996. An ELISA for porcine reproductive and respiratory syndrome: Production of antigen of high quality. Can. J. Vet. Res. 60, 89-93.
4. Chu, J.Q., M.C. Kim, C.S. Park, M.J. You, and M.H. Jun. 2008. Genotypic diversity of the complete open-reading frame 7 sequences of porcine reproductive and respiratory syndrome viruses in Korea and coexistence of two genotypes. J. Vet. Clin. 25, 139-145.
5. Collins, J.E., D.A. Benfield, W.T. Christianson, L. Harris, J.C. Hennings, D.P. Shaw, S.M. Goyal, S. McCullough, R.B. Morrison, H.S. Joo, D. Gorcyca, and D. Chladek. 1992. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) from the disease in gnotobiotic pigs. J. Vet. Diagn. Invest. 4, 117-126.
6. Dea, S., C.A. Gagnon, H. Mardassi, B. Pirzadeh, and D. Rogan. 2000a. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: Comparison of the North American and European isolates. Arch. Virol. 145, 659-688.
7. Dea, S., L. Wilson, D. Therrien, and E. Cornaglia. 2000b. Competitive ELISA for detection of antibodies to porcine reproductive and respiratory syndrome virus using recombinant E. coli-expressed nucleocapsid protein as antigen. J. Virol. Methods 87, 109-122.
8. Denac, H., C. Moser, J.D. Tratschin, and M.A. Hofmann. 1997. An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen. J. Virol. Methods 65, 169-181.
9. Ferrin, N.H., Y. Fang, C.R. Johnson, M.P. Murtaugh, D.D. Polson, M. Torremorell, M.L. Gramer, and E.A. Nelson. 2004. Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 11, 503-514.
10. Greiner, M. 1995. Two-graph receiver operating characteristic (TG-ROC): A Microsoft-EXCEL template for the selection of cutoff values in diagnostic tests. J. Immunol. Methods 185, 145-146.
11. Greiner, M., D. Pfeiffer, and R.D. Smith. 2000. Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests. Prev. Vet. Med. 45, 23-41.
12. Greiner, M., D. Sohr, and P. Goebel. 1995. A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. J. Immunol. Methods 185, 123-132.
13. Jacobson, R.H. 1998. Validation of serological assay for diagnosis of infections disease. Rev. Sci. Tech. 17, 469-526.
14. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Livingston, R.S., D.G. Besselsen, E.K. Steffen, C.L. Besch-Williford, C.L. Franklin, and L.K. Riley. 2002. Serodiagnosis of mice minute virus and mouse parvovirus infections in mice by enzyme-linked immunosorbent assay with baculovirus-expressed recombinant VP2 proteins. Clin. Diagn. Lab. Immunol. 9, 1025-1031.
16. Mardassi, H., S. Mounir, and S. Dea. 1995. Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus, Quebec reference strain. Arch. Virol. 140, 1405-1418.
17. Meng, X.J., P.S. Paul, P.G. Halbur, and M.A. Lum. 1995. Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): Implication for the existence of two genotypes of PRRSV in the USA and Europe. Arch. Virol. 140, 745-755.
18. Mengeling, W.L. and K.M. Lager. 2000. A brief review of procedures and potential problems associated with the diagnosis of porcine reproductive and respiratory syndrome. Vet. Res. 31, 61-69.
19. Meulenberg, J.J., R.J. Bende, J.M.A. Pol, G. Wensvoort, and R.J.M. Moorman. 1995a. Nucleocapsid protein of Lelystad virus: Expression by recombinant baculovirus, immunological properties, and suitability for the detection of serum antibodies. Clin. Diagn. Lab. Immunol. 2, 652-656.
20. Meulenberg, J.J., A. Petersen-Den Besten, E.P. de Kluyer, R.J. Moormann, W.M. Schaaper, and G. Wensvoort. 1995b. Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206, 155-163.
21. Murtaugh, M.P., M.R. Elam, and L.T. Kakach. 1995. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch. Virol. 140, 1451-1460.
22. Nelsen, C.J., M.P. Murtaugh, and K.S. Faaberg. 1999. Porcine reproductive and respiratory syndrome virus comparison: Divergent evolution on two continents. J. Virol. 73, 99-118.
23. Oleksiewicz, M.B, T. Stadejek, Z. MaĆkiewicz, M. Porowski, and Z. Pejsak. 2005. Discriminating between serological responses to European-genotype live vaccine and European-genotype field strains of porcine reproductive and respiratory syndrome virus (PRRSV) by peptide ELISA. J. Virol. Methods 129, 134-144.
24. Plagemann, P.G.W. 2006. Peptide ELISA for measuring antibodies to N-protein of porcine reproductive and respiratory syndrome virus. J. Virol. Methods 134, 99-118.
25. Seuberlich, T., J.D. Tratschin, B. Thür, and M.A. Hofmann. 2002. Nucleocapsid protein-based enzyme-linked immunosorbent assay for detection and differentiation of antibodies against European and North American porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 9, 1183-1191.
26. Takikawa, N., S. Kobayashi, S. Ide, Y. Yamane, Y. Tanaka, and H. Yamagishi. 1996. Detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus in swine sera by enzyme-linked immunosorbent assay. J. Vet. Med. Sci. 58, 355-357.
27. Wensvoort, G., E.P. de Kluyver, J.M. Pol, F. Wagenaar, R.J. Moormann, M.M. Hulst, R. Bloemraad, A. den Besten, T. Zetstra, and C. Terpstra. 1992. Lelystad virus, the cause of porcine epidemic abortion and respiratory syndrome: A review of mystery swine disease research at Lelystad. Vet. Microbiol. 33, 185-193.
28. Wensvoort, G., C. Terpstra, J.M. Pol, E.A. Ter Laak, M. Bloemraad, E.P. De Kluyver, C. Kragten, L. van Buiten, A. Den Besten, F. Wagenaar, J.M. Broekhuijsen, P.L.J.M.T. Moonen, Zetstra, E.A. De Boer, H.J. Tibben, M.F. De Jong, P. Vant Veld, G.J.R. Groenland, J.A. van Gennep, M.T. Voets, J.H.M. Verheijden, and J. Braamskamp. 1991. Mystery swine disease in the Netherlands: The isolation of Lelystad virus. Vet. Q. 13, 121-130.
29. Yoon, I.J., H.S. Joo, W.T. Christianson, H.S. Kim, J.E. Collins, R.B. Morrison, and G.D. Dial. 1992. An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J. Vet. Diagn. Invest. 4, 144-147.