High-level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation

Applied and Environmental Microbiology

Volumn 75, Issue 21, 2009, Pages 6886-6889

  Fenoy, S ^a   Rueda, C ^a   Higes, M ^b   Martin Hernandez R ^b   Del Aguila, C ^a  

^a UNIVERSIDAD SAN PABLO CEU   (Spain)

^b Centro Apicola Regional   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EXPOSURE CONDITIONS; PARASITE-; THERMOTOLERANCE;

4',6 DIAMIDINO 2 PHENYLINDOLE;

DESICCATION; FREEZING; HONEYBEE; PARASITE RESISTANCE; SPORE; TEMPERATURE EFFECT;

ARTICLE; DEGENERATION; DESICCATION; FREEZING; HEAT TOLERANCE; HONEYBEE; NONHUMAN; NOSEMA; NOSEMA CERANAE;

ANIMALS; BEES; DESICCATION; FREEZING; HOT TEMPERATURE; INDOLES; MICROBIAL VIABILITY; NOSEMA; ORGANIC CHEMICALS; SPORES, FUNGAL; STAINING AND LABELING; STRESS, PHYSIOLOGICAL;

APIS MELLIFERA; NOSEMA CERANAE;

EID: 70350513610     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01025-09     Document Type: Article

References (18)

1. Amigo, J. M., M. P. Gracia, M. Rius, H. Salvado, P. A. Maillo, and C. P. Vivares. 1996. Longevity and effects of temperature on the viability and polar-tube extrusion of spores of Glugea stephani, a microsporidian parasite of commercial flatfish. Parasitol. Res. 82:211-214.
2. Cantwell, G. E., and H. Shimanuki. 1970. The use of heat to control Nosema and increase production for the commercial beekeeper. Am. Bee J. 110:263.
3. Chen, Y., J. D. Evans, I. B. Smith, and J. S. Pettis. 2008. Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. J. Invertebr. Pathol. 97: 186-188.
4. Fries, I., R. Martín, A. Meana, P. García-Palencia, and M. Higes. 1996. Nosema ceranae n. sp. (Microspora, Nosematidae). Morphological and molecular characterization of microsporidian parasite of the Asian honey bees Apis cerana (Hymenoptera, Apidae). Eur. J. Protistol. 32:356-365. (Pubitemid 26380633)
5. Green, L. C., P. J. LeBlanc, and E. S. Didier. 2000. Discrimination between viable and dead Encephalitozoon cuniculi (microsporidian) spores by dual staining with Sytox Green and Calcofluor White M2R. J. Clin. Microbiol. 38:3811-3814.
6. Higes, M., P. Garcia-Palencia, R. Martin-Hernandez, and A. Meana. 2007. Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia). J. Invertebr. Pathol. 94:211-217. (Pubitemid 46240071)
7. Higes, M., R. Martin-Hernandez, C. Botias, E. G. Bailon, A. V. Gonzalez- Porto, L. Barrios, M. J. Del Nozal, J. L. Bernal, J. J. Jimenez, P. G. Palencia, and A. Meana. 2008. How natural infection by Nosema ceranae causes honeybee colony collapse. Environ. Microbiol. 10:2659-2669.
8. Higes, M., R. Martin-Hernandez, E. Garrido-Bailon, C. Botias, P. Garcia- Palencia, and A. Meana. 2008. Regurgitated pellets of Merops apiaster as fomites of infective Nosema ceranae (Microsporidia) spores. Environ. Microbiol. 10:1374-1379.
9. Higes, M., R. Martin, and A. Meana. 2006. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol. 92:93-95.
10. Koudela, B., S. Kucerova, and T. Hudcovic. 1999. Effect of low and high temperatures on infectivity of Encephalitozoon cuniculi spores suspended in water. Folia Parasitol. (Praha) 46:171-174.
11. Malone, L. A., H. S. Gatehouse, and E. L. Tregidga. 2001. Effects of time, temperature, and honey on Nosema apis (Microsporidia: Nosematidae), a parasite of the honeybee, Apis mellifera (Hymenoptera: Apidae). J. Invertebr. Pathol. 77:258-268.
12. Martín-Hernández, R., A. Meana, L. Prieto, A. M. Salvador, E. Garrido- Bailon, and M. Higes. 2007. Outcome of colonization of Apis mellifera by Nosema ceranae. Appl. Environ. Microbiol. 73:6331-6338.
13. Martín-Hernández, R., A. Meana, P. García-Palenci, P. Marín, C. Botías, E. Garrido-Bailón, L. Barrios, and M. Higes. 2009. Effect of temperature on the biotic potential of honeybee microsporidia. Appl. Environ. Microbiol. 75:2554-2557.
14. Oldroyd, B. P. 2007. What's killing American honey bees? PLoS Biol. 5:1195-1199.
15. Paxton, R. J. K., J. S. Korpela, and I. Fries. 2007. Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 38:1-9.
16. Santillana-Hayat, M., C. Sarfati, S. Fournier, F. Chau, R. Porcher, J. M. Molina, and F. Derouin. 2002. Effects of chemical and physical agents on viability and infectivity of Encephalitozoon intestinalis determined by cell culture and flow cytometry. Antimicrob. Agents Chemother. 46:2049-2051.
17. Shadduck, J. A., and M. B. Polley. 1978. Some factors influencing the in vitro infectivity and replication of Encephalitozoon cuniculi. J. Protozool. 25:491-496.
18. Zander, E. 1909. Tierische Parasiten als Krankheitserreger bei der Biene. Leipziger Bienenztg. 24:147-150, 163-166.