Flow cytometric analysis of age-related changes in intestine intraepithelial lymphocyte subsets and their functional preservation after feeding mice on spirulina

Journal of Medicinal Food

Volumn 12, Issue 5, 2009, Pages 982-989

  Hayashi, Osamu ^a   Katayanagi, Yuki ^a   Ishii, Kyoko ^a   Kato, Toshimitsu ^b  

^a KAGAWA NUTRITION UNIVERSITY   (Japan)

^b DIC CORPORATION   (Japan)

Author keywords

Aging; Cyanobacteria; Flow cytometric assay; Intraepithelial lymphocytes

Indexed keywords

CD45 ANTIGEN; CD8 ANTIGEN; CELL MARKER; HOT WATER; MEMBRANE ANTIGEN; PROBIOTIC AGENT;

ADULT ANIMAL; AGED; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL COUNT; CONTROLLED STUDY; FEEDING; FEMALE; FLOW CYTOMETRY; INTESTINE EPITHELIUM; LYMPHOCYTE SUBPOPULATION; MOUSE; NONHUMAN; PRESERVATION; PRIORITY JOURNAL; SPIRULINA;

AGING; ANIMALS; ANTIGENS, CD45; BIOLOGICAL PRODUCTS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; FEMALE; FLOW CYTOMETRY; INTESTINAL MUCOSA; INTESTINE, SMALL; MICE; MICE, INBRED BALB C; PROTECTIVE AGENTS; RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; SPIRULINA; T-LYMPHOCYTE SUBSETS;

CYANOBACTERIA; MUS; SPIRULINA;

EID: 70350507940     PISSN: 1096620X     EISSN: None     Source Type: Journal    
DOI: 10.1089/jmf.2008.1260     Document Type: Article

References (31)

1. Prelog M: Aging of the immune system: a risk factor for autoimmunity? Autoimmun Rev 2006;5:136-139.
2. Solana R, Pawelec G, Tarazona R: Aging and innate immunity. Immunity 2006;24:491-494.
3. Hayashi O, Murakami N, Ishii K, Miyaji N: Influence of astaxanthin ingestion on the mucosal immune responses in adult and aged mice. J Phys Fit Nutr Immunol 2007;17:12-24.
4. Miquel J: Nutrition and ageing. Public Health Nutr 2001;4:1385-1388.
5. De la Fuente M: Effects of antioxidants on immune system ageing. Eur J Clin Nutr 2002;56:55-58.
6. Koga T, McGhee JR, Kato H, et al.: Evidence for early aging in the mucosal immune system. J Immunol 2000;165:5352-5359.
7. Cunningham-Rundles S: The effect of aging on mucosal host defense. J Nutr Health Aging 2004;8:20-25.
8. Krause D, Mastro AM, Handte G, Smiciklas-Wright H, Miles MP, Ahluwalia N: Immune function did not decline with aging in apparently healthy, well-nourished women. Mech Ageing Dev 1999;112:43-57.
9. Schmucker DL, Owen RL, Outenreath R, Thoreux K: Basis for the age-related decline in intestinal mucosal immunity. Clin Dev Immunol 2003;10:167-172.
10. Hayday A, Theodoridis E, Ramsburg E, Shires J: Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol 2001;2:997-1003.
11. Belay A: Spirulina (Arthrospira): production and quality assurance. In: Spirulina in Human Nutrition and Health (Gershwin ME, Belay A, eds.). CRC Press Taylor & Francis Group, Boca Raton, FL, 2007 pp. 1-25.
12. Nemoto-Kawamura C, Hirahashi T, Nagai T, Yamada H, Katoh T, Hayashi O: Phycocyanin enhances secretory IgA antibody responses and suppresses allergic IgE antibody response in mice immunized with antigen-entrapped biodegradable microparticles. J Nutr Sci Vitaminol (Tokyo) 2004;50:129-136.
13. Hayashi O, Ono S, Ishii K, et al.: Enhancement of proliferation and differentiation in bone marrow hematopoietic cells by Spirulina (Arthrospira) platensis in mice. J Appl Phycol 2006;18:47-56.
14. Hayashi O, Hirahashi T, Katoh T, Miyajima H, Hirano T, Okuwaki Y: Class specific influence of dietary Spirulina platensis on antibody production in mice. J Nutr Sci Vitaminol (Tokyo) 1998; 44:841-851.
15. Mosely RL, Klein JR: A rapid method for isolating murine intestine intraepithelial lymphocytes with high yield and purity. J Immunol Methods 1992;156:19-26.
16. Montufar-Solis D, Klein JR: An improved method for isolating intraepithelial lymphocytes (IELs) from the murine small intestine with consistently high purity. J Immunol Methods 2006; 308:251-254.
17. Lydyard P, Grossi C: Lymphoid system. In: Immunology, 5th ed. (Roitt I, Brostoff J, Male D, eds.). Mosby International Ltd., London, 1998, pp. 31-41.
18. von Ewijk W, Hayday A, Merkenschlager M, et al.: The thymus and the development of T lymphocytes. In: Immunobiology-The Immune System in Health and Disease, 4th ed. (Janeway CA, Travers P, Walport M, Capra JD, eds.). Grand Publishing, New York, 1999, pp. 227-260.
19. Nanno M, Onai N, Ishikawa H: [Development and physiological functions of intraepithelial lymphocytes in the intestinal mucosa]. Tanpakushitsu Kakusan Koso 2002;47(16 Suppl):2318-2324.
20. Rocha B, Vassalli P, Guy-Grand D: Thymic and extrathymic origins of gut intraepithelial lymphocyte populations in mice. J Exp Med 1994;180:681-686.
21. Nanno M: Development and physiological functions of intestinal intraepithelial lymphocytes. Cell 2004;36:365-368.
22. Lefrancois L: Phenotypic complexity of intraepithelial lymphocytes of small intestine. J Immunol 1991;147:1746-1751.
23. Komuro T, Sano K, Asano Y, Tada T: Analysis of aged-related degeneracy of T-cell repertoire: localized functional failure in CD8+ T cells. Scand J Immunol 1990;32:545-553.
24. Takimoto H, Nakamura T, Takeuchi M, et al.: Age-associated increase in number of CD4+CD8+ intestinal intraepithelial lymphocytes in rats. Eur J Immunol 1992;22:159-164.
25. Cruse JM, Lewis RE, eds.: Illustrated Dictionary of Immunology. CRC Press, Boca Raton, FL, 1995.
26. Komano H, Fujiura Y, Kawaguchi M, et al.: Homeostatic regulation of intestinal epithelia by intraepithelial gamma delta T cells. Proc Natl Acad Sci U S A 1995;92:6147-6151.
27. Zhang C-W: Effect of polysaccharide and phycocyanin from Spirulina on peripheral blood and hematopoietic system of bone marrow in mice. In: Proceeding of 2nd Asia Pacific Conference on Algal Biotechnology, China. Bioline International, Centro de Referência em Informação Ambiental CRIA, Barão Geraldo, Brazil, 1994, p. 58.
28. González R, Rodríguez S, Romay C, et al.: Anti-inflammatory activity of phycocyanin extract in acetic acid-induced colitis in rats. Pharmacol Res 1999;39:55-59.
29. Vila J, Gemma C, Bachstetter A, et al.: Spirulina, aging, and neurobiology. In: Spirulina in Human Nutrition and Health (Gershwin ME, Belay A, eds.). CRC Press Taylor & Francis Group, Boca Raton, FL, 2007, pp. 271-291.
30. Park EJ, Takahashi I, Ikeda J, et al.: Clonal expansion of doublepositive intraepithelial lymphocytes by MHC class I-related chain A expressed in mouse small intestinal epithelium. J Immunol 2003;171:4131-4139.
31. Kim JK, Takahashi I, Kai Y, Kiyono H: Influence of enterotoxin on mucosal intranet: selective inhibition of extrathymic T cell development in intestinal intraepithelial lymphocytes by oral exposure to heat-labile toxin. Eur J Immunol 2001;31:2960-2969.