A relatively simple and economical protocol for proteomic analyses of human 20S proteasome: Compatible with both scaled-up and scaled-down purifications

Electrophoresis

Volumn 30, Issue 14, 2009, Pages 2422-2430

  Chen, Guoqiang ^a   Luo, Yuanming ^b   Wang, Xinli ^a   Zhao, Zhiyun ^a   Liu, Hui ^a   Zhang, Haijing ^a   Li, Zhili ^a  

^a Postdoctoral Research Station Institute of Basic Medicine Sciences CAMS and PUMC   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Differential centrifugation; Direct 3 DE; Native gel electrophoresis; Proteasome purification

Indexed keywords

CENTRIFUGATION; ECONOMIC ANALYSIS; ELECTROPHORESIS; LIQUID CHROMATOGRAPHY;

CORE PARTICLES; DIFFERENTIAL CENTRIFUGATION; DIRECT 3-DE; HEPTAMERIC RINGS; NATIVE GEL ELECTROPHORESIS; PROTEASOME PURIFICATION; PROTEASOMES; PROTEOMIC ANALYSIS; SCALED-UP; SIMPLE++;

PURIFICATION;

PROTEASOME;

ARTICLE; CANCER CELL; CANCER CELL CULTURE; CENTRIFUGATION; CONTROLLED STUDY; DIFFUSION; ECONOMICS; ERYTHROCYTE; GEL ELECTROPHORESIS; HUMAN; PANCREAS CANCER; PARTICLE SIZE; PROTEIN PURIFICATION; PROTEOMICS; BLOOD; CHEMISTRY; METHODOLOGY; PANCREAS TUMOR; POLYACRYLAMIDE GEL ELECTROPHORESIS; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE;

CELL LINE, TUMOR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; HUMANS; PANCREATIC NEOPLASMS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEOMICS; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY;

EID: 70350442776     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800802     Document Type: Article

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