2-D difference in gel electrophoresis combined with Pro-Q Diamond staining: A successful approach for the identification of kinase/phosphatase targets

Electrophoresis

Volumn 30, Issue 14, 2009, Pages 2469-2476

  Orsatti, Laura ^a   Forte, Eleonora ^b   Tomei, Licia ^a   Caterino, Marianna ^c   Pessi, Antonello ^a   Talamo, Fabio ^a  

^a IRBM   (Italy)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CEINGE BIOTECNOLOGIE AVANZATE   (Italy)

Author keywords

2 D difference in gel electrophoresis; Elongation factor 2; Ezrin; Phosphorylation; PRL 3

Indexed keywords

AMINO ACIDS; CANCER CELLS; DIAMONDS; ELECTROPHORESIS; ENDOTHELIAL CELLS; PATHOLOGY; PHOSPHATASES; PHOSPHORYLATION;

2-D DIFFERENCE IN GEL ELECTROPHORESIS; ELONGATION FACTOR; ELONGATION FACTOR 2; EZRIN; GEL ELECTROPHORESIS; MODELLING SYSTEMS; PRL-3; PROTEIN TARGETS; PROTEIN-TYROSINE PHOSPHATASE; TUMORIGENESIS;

DISEASES;

EZRIN; PROTEIN TYROSINE PHOSPHATASE; CYTOSKELETON PROTEIN; ELONGATION FACTOR 2; ORGANOMETALLIC COMPOUND; PHOSPHOPROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN KINASE; PTP4A3 PROTEIN, HUMAN; SYPRO RUBY; TUMOR PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CANCER CELL; CANCER GROWTH; CARCINOGENESIS; CELL STRAIN HCT116; COLON CANCER; CONTROLLED STUDY; GEL ELECTROPHORESIS; GENE OVEREXPRESSION; GENE SEQUENCE; HUMAN; HUMAN CELL; MASS SPECTROMETRY; METASTASIS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; PROTEIN ANALYSIS; PROTEIN TARGETING; SEQUENCE ANALYSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS; VASCULAR ENDOTHELIUM; METABOLISM; METHODOLOGY; STAINING; TUMOR CELL LINE;

CELL LINE, TUMOR; CYTOSKELETAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HCT116 CELLS; HUMANS; NEOPLASM PROTEINS; ORGANOMETALLIC COMPOUNDS; PEPTIDE ELONGATION FACTOR 2; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN KINASES; PROTEIN TYROSINE PHOSPHATASES; STAINING AND LABELING;

EID: 70350442773     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800780     Document Type: Article

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