Evaluation of DNA colony hybridization and real-time PCR for detection of Vibrio parahaemolyticus and Vibrio vulnificus in postharvest-processed oysters

Journal of Food Protection

Volumn 72, Issue 10, 2009, Pages 2106-2109

  Jones, Jessica L ^a   Noe, Kathy E ^a   Byars, Robin ^a   Depaola, Angelo ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OSTREIDAE; VIBRIO; VIBRIO PARAHAEMOLYTICUS; VIBRIO VULNIFICUS;

EID: 70350300641     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-72.10.2106     Document Type: Article

References (7)

1. Blackstone, G. M., J. L. Nordstrom, M. C. Vickery, M. D. Bowen, R. F. Meyer, and A. DePaola. 2003. Detection of pathogenic Vibrio parahaemolyticus in oyster enrichments by real time PCR. J. Microbiol. Methods 53: 149-155. (Pubitemid 36332349)
2. Campbell, M. S., and A. C. Wright. 2003. Real-time PCR analysis of Vibrio vulnificus from oysters. Appl. Environ. Microbiol. 69: 7137-7144. (Pubitemid 38585362)
3. DePaola, A., J. L. Jones, K. E. Noe, R. H. Byars, and J. C. Bowers. 2009. Survey of postharvest-processed oysters in the United States for levels of Vibrio vulnificus and Vibrio parahaemolytkus. J. Food Prot. 72: 2110-2113.
4. Kaysner, C. A., and A. DePaola. 2004. Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp., chap. 9. In Bacteriological analytical manual, 9th ed. Association of Official Analytical Chemists, Arlington, VA.
5. McCarthy, S. A., A. DePaola, D. W. Cook, C. A. Kaysner, and W. E. Hill. 1999. Evaluation of alkaline phosphatase- and digoxigenin-labelled probes for detection of the thermolabile hemolysin (tlh) gene of Vibrio parahaemolyticus. Lett. Appl. Microbiol. 28: 66-70. (Pubitemid 29051841)
6. Nordstrom, J. L., M. C. L. Vickery, G. M. Blackstone, S. L. Murray, and A. DePaola. 2007. Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters. Appl. Environ. Microbiol. 73: 5840-5847. (Pubitemid 47462996)
7. Wright, A. C., G. A. Miceli, W. L. Landry, J. B. Christy, W. D. Watkins, and J. G. Morris, Jr. 1993. Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphataselabeled oligonucleotide probe. Appl. Environ. Microbiol. 59: 541-546.