PCR-based cloning of the complete mouse mitochondrial genome and stable engineering in escherichia coli

Biotechnology Letters

Volumn 31, Issue 11, 2009, Pages 1671-1676

  Yoon, Young Geol ^a   Yang, Yi Wei ^b   Koob, Michael D ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

ori replication; Mitochondrial genome; mtDNA cloning; Sequential ligation

Indexed keywords

COPY NUMBER; DNA POLYMERASE; DNA REPLICATIONS; E. COLI; HIGH FIDELITY; MITOCHONDRIAL GENOME; MITOCHONDRIAL GENOMES; MTDNA CLONING; NUCLEOTIDE SEQUENCING; RESTRICTION ENZYMES; SEQUENTIAL LIGATION;

AMPLIFICATION; CELL MEMBRANES; DNA; ESCHERICHIA COLI; GENES; MAMMALS; NUCLEIC ACIDS;

CLONING;

MITOCHONDRIAL DNA;

ANIMAL; ARTICLE; CELL CLONE; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MITOCHONDRIAL GENOME; MOLECULAR CLONING; MOUSE; POLYMERASE CHAIN REACTION;

ANIMALS; CLONE CELLS; CLONING, MOLECULAR; DNA, MITOCHONDRIAL; ESCHERICHIA COLI; GENETIC ENGINEERING; GENOME, MITOCHONDRIAL; MICE; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI; MAMMALIA;

EID: 70350263429     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0063-9     Document Type: Article

References (12)

1. Chang DD, Clayton DA (1986) Identification of primary transcriptional start sites of mouse mitochondrial DNA: accurate in vitro initiation of both heavy- and light-strand transcripts. Mol Cell Biol 6:1446-1453.
2. DiMauro S, Schon EA (2008) Mitochondrial disorders in the nervous system. Annu Rev Neurosci 31:91-123.
3. Gilbert MT et al (2007) Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. Science 317:1927-1930.
4. Greener A, Filutowicz MS, McEachern MJ, Helinski DR (1990) N-terminal truncated forms of the bifunctional π initiation protein express negative activity on plasmid R6K replication. Mol Gen Genet 224:24-32.
5. Ioannou PA, Amemiya CT, Garnes J, Kroisel PM, Shizuya H, Chen C, Batzer MA, de Jong PJ (1994) A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet 6:84-89.
6. Kearsey SE, Flanagan JG, Craig IW (1980) Cloning of mouse mitochondrial DNA in E. coli affects bacterial viability. Gene 12:249-255.
7. Kim UJ, Birren BW, Slepak T, Mancino V, Boysen C, Kang HL, Simon MI, Shizuya H (1996) Construction and characterization of a human bacterial artificial chromosome library. Genomics 34:213-218.
8. Koob M, Shaw A, Cameron DC (1994) Minimizing the genome of Escherichia coli. Motivation and strategy. Ann N Y Acad Sci 745:1-3.
9. Krause J, Dear PH, Pollack JL, Slatkin M, Spriggs H, Barnes I, Lister AM, Ebersberger I, Pääbo S, Hofreiter M (2006) Multiplex amplification of the mammoth mitochondrial genome and the evolution of Elephantidae. Nature 439:724-727.
10. Thr gene: reversion of an 'uncloneable' phenotype. Mutat Res 199:183-190.
11. Pfanner N, Geissler A (2001) Versatility of the mitochondrial protein import machinery. Nat Rev Mol Cell Biol 2:339-349.
12. Yoon YG, Koob MD (2003) Efficient cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into transcriptionally active mitochondria. Nucleic Acids Res 31:1407-1415.