Purification of Schwann cells from adult rats by differential detachment

Biotechnology Letters

Volumn 31, Issue 11, 2009, Pages 1703-1708

  Wu, Wei ^a,b   Jin, Yu Qing ^a,b   Kretlow, James D ^c   Xu, Liang ^a,b   Duan, Hui Chuan ^a,b   Qi, Zuo Liang ^a  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b Shanghai Tissue Engineering Key Laboratory   (China)

^c Rice University   (United States)

Author keywords

Collagenase; Differential detachment; Nerve regeneration; Purification; Schwann cells

Indexed keywords

ADULT ANIMALS; BEFORE AND AFTER; CELL YIELDS; CLINICAL APPLICATION; COLLAGENASE; DIFFERENTIAL DETACHMENT; EFFICIENT METHOD; FLOW-CYTOMETRIC ANALYSIS; IMMUNOSTAINING; INDEPENDENT ANALYSIS; NERVE REGENERATION; SCHWANN CELLS; SYSTEMATIC BIAS;

ANIMALS; CELL MEMBRANES; RATS;

PURIFICATION;

AGING; ANIMAL; ARTICLE; CELL ADHESION; CELL CULTURE; CELL SEPARATION; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GROWTH, DEVELOPMENT AND AGING; MALE; METHODOLOGY; ORGAN SIZE; PHYSIOLOGY; RAT; SCHWANN CELL; SCIATIC NERVE; WISTAR RAT;

AGING; ANIMALS; CELL ADHESION; CELL SEPARATION; CELLS, CULTURED; FLUORESCENT ANTIBODY TECHNIQUE; MALE; ORGAN SIZE; RATS; RATS, WISTAR; SCHWANN CELLS; SCIATIC NERVE;

ANIMALIA; RATTUS;

EID: 70350255732     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0064-8     Document Type: Article

References (14)

1. Aszmann OC (2000) The life and work of Theodore Schwann. J Reconstr Microsurg 16:291-295.
2. Bhatheja K, Field J (2006) Schwann cells: origins and role in axonal maintenance and regeneration. Int J Biochem Cell Biol 38:1995-1999.
3. Brockes JP, Fields KL, Raff MC (1979) Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve. Brain Res 165:105-118.
4. Casella GT, Bunge RP, Wood PM (1996) Improved method for harvesting human Schwann cells from mature peripheral nerve and expansion in vitro. Glia 17:327-338.
5. Fu SY, Gordon T (1997) The cellular and molecular basis of peripheral nerve regeneration. Mol Neurobiol 14:67-116.
6. Jin YQ, Liu W, Hong TH et al (2008) Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment. J Neurosci Methods 170:140-148.
7. Keilhoff G, Fansa H, Schneider W et al (1999) In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits. J Neurosci Methods 89:17-24.
8. Komiyama T, Nakao Y, Toyama Y et al (2003) A novel technique to isolate adult Schwann cells for an artificial nerve conduit. J Neurosci Methods 122:195-200.
9. Mosahebi A, Woodward B, Wiberg M et al (2001) Retroviral labeling of Schwann cells: in vitro characterization and in vivo transplantation to improve peripheral nerve regeneration. Glia 34:8-17.
10. Oudega M, Xu XM (2006) Schwann cell transplantation for repair of the adult spinal cord. J Neurotrauma 23:453-467.
11. Pannunzio ME, Jou IM, Long A et al (2005) A new method of selecting Schwann cells from adult mouse sciatic nerve. J Neurosci Methods 149:74-81.
12. Tohill MP, Mann DJ, Mantovani CM et al (2004) Green fluorescent protein is a stable morphological marker for Schwann cell transplants in bioengineered nerve conduits. Tissue Eng 10:1359-1367.
13. Vroemen M, Weidner N (2003) Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve. J Neurosci Methods 124:135-143.
14. Woodhoo A, Sommer L (2008) Development of the Schwann cell lineage: from the neural crest to the myelinated nerve. Glia 56:1481-1490.