Rapid selection of peptide containing fractions in off-line 2-D HPLC in shotgun proteome analysis by screening with MALDI TOF MS

Proteomics

Volumn 9, Issue 19, 2009, Pages 4577-4581

  Lasaosa, Maria ^a   Jakoby, Thomas ^a,b   Delmotte, Nathanaël ^a   Huber, Christian G ^a,c   Heinzle, Elmar ^a   Tholey, Andreas ^b  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY OF KIEL   (Germany)

^c UNIVERSITY OF SALZBURG   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PEPTIDE; PROTEOME;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION PAIR CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SCREENING; SEPARATION TECHNIQUE;

BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; PEPTIDES; PROTEOME; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

BACTERIA (MICROORGANISMS);

EID: 70350228779     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200900274     Document Type: Article

References (8)

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5. Delmotte, N., Lasaosa, M., Tholey, A., Heinzle, E. et al., Repeatability of peptide identifications in shotgun proteome analysis employing off-line two-dimensional-chromato-graphic separations and ion-trap mass spectrometry. J. Sep. Sci. 2009, 32, 1156-1164.
6. Lasaosa, M., Delmotte, N., Huber, C. G. Melchior, K. et al., A 2D-reversed-phase x ion-pair reversed-phase HPLC MALDI TOF/TOF MS approach for shotgun proteome analysis. Anal. Bioanal. Chem. 2009, 393, 1245-1256.
7. Zabet-Moghaddam, M., Heinzle, E., Lasaosa, M., Tholey, A., Pyridinium based ionic liquid matrices can improve the identification of proteins by peptide mass fingerprint analysis with matrix assisted laser desorption/ionization mass spectrometry. Anal. Bioanal. Chem. 2006, 384, 215-224.
8. Kratzer, R., Eckerskorn, C., Karas, M., Lottspeich, F., Suppression effects in enzymatic peptide ladder sequencing using ultraviolet - matrix assisted laser desorption/ionization - mass spectrometry. Electrophoresis 1998, 19, 1910-1919. (Pubitemid 28392236)