Crystal Structures of A. acidocaldarius Endoglucanase Cel9A in Complex with Cello-Oligosaccharides: Strong - 1 and - 2 Subsites Mimic Cellobiohydrolase Activity

Journal of Molecular Biology

Volumn 394, Issue 1, 2009, Pages 61-70

  Eckert, Kelvin ^a   Vigouroux, Armelle ^a   Lo Leggio, Leila ^b   Moréra, Solange ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

cellobiose complex; cellotetraose complex; glycosylase GH9; X ray crystallography

Indexed keywords

CELLOBIOSE; CELLULASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; GLUCAN SYNTHASE; GLUCOSE; GLYCOSIDASE; LIGAND; OLIGOSACCHARIDE; POLYMER;

ALICYCLOBACILLUS ACIDOCALDARIUS; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ALICYCLOBACILLUS ACIDOCALDARIUS;

ARCHAEA; ARCHAEAL PROTEINS; BINDING SITES; BIOCATALYSIS; CELLOBIOSE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CRYSTALLOGRAPHY, X-RAY; LIGANDS; METALS; MOLECULAR MIMICRY; PROTEIN STRUCTURE, SECONDARY; STATIC ELECTRICITY; SUBSTRATE SPECIFICITY; TETROSES;

EID: 70350156419     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2009.08.060     Document Type: Article

References (24)

1. Cantarel B.L., Coutinho P.M., Rancurel C., Bernard T., Lombard V., and Henrissat B. The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 37 (2009) D233-D238
2. Schmidt A., Gubitz G.M., and Kratky C. Xylan binding subsite mapping in the xylanase from Penicillium simplicissimum using xylooligosaccharides as cryo-protectant. Biochemistry 38 (1999) 2403-2412
3. Koshland D.E. Stereochemistry and the mechanism of enzymatic reactions. Biol. Rev. 28 (1953) 416-436
4. Sinnott M.L. Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90 (1991) 1170-1202
5. Davies G.J., Wilson K.S., and Henrissat B. Nomenclature for sugar-binding subsites in glycosyl hydrolases. Biochem. J. 321 (1997) 557-559
6. Bayer E.A., Shoham Y., and Lamed R. Cellulose-decomposing prokaryotes and their enzyme systems. In: Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., and Stackebrandt E. (Eds). The Prokaryotes: An Involving Electronic Resource for the Microbial Community (2000), Springer Verlag, New York, NY
7. Parsiegla G., Belaich A., Belaich J.P., and Haser R. Crystal structure of the cellulase Cel9M enlightens structure/function relationships of the variable catalytic modules in glycoside hydrolases. Biochemistry 41 (2002) 11134-11142
8. Sakon J., Irwin D., Wilson D.B., and Karplus P.A. Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nat. Struct. Biol. 4 (1997) 810-818
9. Mandelman D., Belaich A., Belaich J.P., Aghajari N., Driguez H., and Haser R. X-ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides. J. Bacteriol. 185 (2003) 4127-4135
10. Juy M., Amit A.G., Alzari P.M., Poljak R.J., Claeyssens M., Beguin P., and Aubert J.P. Three-dimensional structure of a thermostable bacterial cellulose. Nature 357 (1992) 89-91
11. Schubot F.D., Kataeva I.A., Chang J., Shah A.K., Ljungdahl L.G., Rose J.P., and Wang B.C. Structural basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum. Biochemistry 43 (2004) 1163-1170
12. Khademi S., Guarino L.A., Watanabe H., Tokuda G., and Meyer E.F. Structure of an endoglucanase from termite, Nasutitermes takasagoensis. Acta Crystallogr., Sect. D: Biol. Crystallogr. 58 (2002) 653-659
13. Beguin P. Molecular biology of cellulose degradation. Annu. Rev. Microbiol. 44 (1990) 219-248
14. Eckert K., Zielinski F., Lo Leggio L., and Schneider E. Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009. Appl. Microbiol. Biotechnol. 60 (2002) 428-436
15. Zheng H., Chruszcz M., Lasota P., Lebioda L., and Minor W. Data mining of metal ion environments present in protein structures. J. Inorg. Biochem. 102 (2008) 1765-1776
16. Schmidt A., Schlacher A., Steiner W., Schwab H., and Kratky C. Structure of the xylanase from Penicillium simplicissimum. Protein Sci. 7 (1998) 2081-2088
17. Rouvinen J., Bergfors T., Teeri T., Knowles J.K., and Jones T.A. Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249 (1990) 380-386
18. Miller J. Experiments in Molecular Genetics (1972), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
19. Eckert K., Ernst H.A., Schneider E., Larsen S., and Lo Leggio L. Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Crystallogr., Sect. D: Biol. Crystallogr. 59 (2003) 139-141
20. Kabsch W. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J. Appl. Crystallogr. 26 (1993) 795-800
21. Collaborative Computational Project, Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr., Sect. D: Biol. Crystallogr. 50 (1994) 760-763
22. McCoy A.J. Solving structures of protein complexes by molecular replacement with Phaser. Acta Crystallogr., Sect. D: Biol. Crystallogr. 63 (2007) 32-41
23. Vagin A.A., Steiner R.A., Lebedev A.A., Potterton L., McNicholas S., Long F., and Murshudov G.N. REFMAC5 dictionary: organization of prior chemical knowledge and guidelines for its use. Acta Crystallogr., Sect. D: Biol. Crystallogr. 60 (2004) 2184-2195
24. Emsley P., and Cowtan K. Coot: model-building tools for molecular graphics. Acta Crystallogr., Sect. D: Biol. Crystallogr. 60 (2004) 2126-2132