Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata

International Journal of Developmental Biology

Volumn 53, Issue 7, 2009, Pages 1057-1062

  Cavaliere, Daniela ^a   Di Cara, Francesca ^b   Polito, Lino C ^a,c   Digilio, Filomena Anna ^a  

^a CNR   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Drosophila melanogaster; Intersex; Maruca vitrata; Sex determination

Indexed keywords

COMPLEMENTARY DNA; GENE PRODUCT; INTERSEX PROTEIN; MESSENGER RNA; UNCLASSIFIED DRUG; DROSOPHILA PROTEIN; INSECT PROTEIN; INTERSEX PROTEIN, DROSOPHILA; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; DNA ISOLATION; DNA SEQUENCE; DROSOPHILA MELANOGASTER; EXPRESSED SEQUENCE TAG; FEMALE; GENE EXPRESSION; LEPIDOPTERA; MALE; MARUCA VITRATA; MOLECULAR CLONING; MUTANT; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SOUTHERN BLOTTING; ALTERNATIVE RNA SPLICING; AMINO ACID SEQUENCE; ANIMAL; COMPARATIVE STUDY; GENE; GENE EXPRESSION PROFILING; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; MOTH; MUTATION; PHYSIOLOGY; SEX DIFFERENTIATION; SILKWORM; SPECIES DIFFERENCE; TRANSGENIC ANIMAL;

DROSOPHILA MELANOGASTER; MARUCA VITRATA;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; BOMBYX; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; FEMALE; GENE EXPRESSION PROFILING; GENES, INSECT; INSECT PROTEINS; MALE; MOLECULAR SEQUENCE DATA; MOTHS; MUTATION; PHENOTYPE; RECOMBINANT PROTEINS; SEX DIFFERENTIATION; SPECIES SPECIFICITY; TRANSCRIPTION FACTORS;

EID: 70350020908     PISSN: 02146282     EISSN: None     Source Type: Journal    
DOI: 10.1387/ijdb.082840dc     Document Type: Article

References (18)

1. BAKER, B.S. and K.A. RIDGE. (1980). Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics 94: 383-423.
2. BRAND, A.H. and N. PERRIMON. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118: 401-415.
3. CHASE, B.A. and B.S. BAKER. (1995). A genetic analysis of intersex, a gene regulating sexual differentiation in Drosophila melanogaster females. Genetics 139: 1649-1661.
4. COSCHIGANO, K.T. and P.C. WENSINK. (1993). Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila. Genes & Development 7: 42-54.
5. CARA, F., R. MORRA, D. CAVALIERE, A. SORRENTINO, A. DE SIMONE, C.L. POLITO, and A.F. DIGILIO. (2006). Structure and expression of a novel gene family showing male germline specific expression in Drosophila melanogaster. Insect molecular biology 15: 813-822.
6. FUJII, T. and T. SHIMADA. (2007). Sex determination in the silkworm, Bombyx mori: a female determinant on the W chromosome and the sex-determining gene cascade. Sem. Cell Dev. Biol.18: 379-388.
7. GARRETT-ENGELE, C.M., M.L. SIEGAL, D.S. MANOLI, B.C. WILLIAMS, H. LI, and B.S. BAKER. (2002). intersex, a gene required for female sexual development in Drosophila, is expressed in both sexes and functions together with doublesex to regulate terminal differentiation. Development 129: 4661-4675.
8. RORTH, P. (1998). Gal4 in the Drosophila female germline. Mech. Dev. 78:113-118.
9. RUBIN, G.M. and A.C. SPRADLING. (1982). Genetic transformation of Drosophila with transposable element vectors. Science 218: 348-353.
10. SACCONE, G., M. SALVEMINI, A. PANE, and L.C. POLITO. (2008). Masculinization of XX Drosophila transgenic flies expressing the Ceratitis capitata DoublesexM isoform. Int. J. Dev. Biol. 52: 1051-1057.
11. SAMBROOK, J., E.F. FRITSCH, and T. MANIATIS. (1989). Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
12. SANCHEZ, L. (2008). Sex-determining mechanisms in insects. Int. J. Dev. Biol. 52: 837-856.
13. SATO, S., C. TOMOMORI-SATO, C.A. BANKS, T.J. PARMELY, I. SOROKINA, C.S. BROWER, R.C. CONAWAY, and J.W. CONAWAY. (2003). A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. J. Biol. Chem. 278: 49671-49674.
14. SIEGAL, M.L. and B.S. BAKER. (2005). Functional conservation and divergence of intersex, a gene required for female differentiation in Drosophila melanogaster. Dev. Genes Evol. 215: 1-12.
15. TRAUT, W., K. SAHARA, and F. MAREC. (2007). Sex chromosomes and sex determination in Lepidoptera. Sex Dev 1: 332-346.
16. WANG, J., Q. XIA, X. HE, M. DAI, J. RUAN, J. CHEN, G. YU, H. YUAN, ET AL. (2005). SilkDB: a knowledgebase for silkworm biology and genomics. Nuc. Acids Res. 33: D399-402.
17. XIA, Q., Z. ZHOU, C. LU, D. CHENG, F. DAI, B. LI, P. ZHAO, X. ZHA, ET AL. (2004). A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306: 1937-1940.
18. YANG, Y., W. ZHANG, J.R. BAYRER, and M.A. WEISS. (2008). Doublesex and the regulation of sexual dimorphism in Drosophila melanogaster: structure, function, and mutagenesis of a female-specific domain. J. Biol. Chem. 283:7280-7292.