Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy

Biochemical and Biophysical Research Communications

Volumn 390, Issue 1, 2009, Pages 16-20

  Ohara Imaizumi, Mica ^a   Aoyagi, Kyota ^a   Akimoto, Yoshihiro ^a   Nakamichi, Yoko ^a   Nishiwaki, Chiyono ^a   Kawakami, Hayato ^a   Nagamatsu, Shinya ^a  

^a KYORIN UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Exocytosis; GLP 1; STC 1 cell; Total internal reflection fluorescence (TIRF) microscopy

Indexed keywords

GLUCAGON LIKE PEPTIDE 1; GLUCOSE; INSULIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL FUSION; CELL LINE; CONTROLLED STUDY; ENDOCRINE CELL; ENTEROENDOCRINE CELL; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; GRANULE CELL; HORMONE RELEASE; MURINAE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL;

ANIMALS; BACTERIAL PROTEINS; CELL LINE; EXOCYTOSIS; GLUCAGON-LIKE PEPTIDE 1; HUMAN GROWTH HORMONE; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; SECRETORY VESICLES;

MURINAE;

EID: 70349954766     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.09.043     Document Type: Article

References (20)

1. Holst J.J. The physiology of glucagon-like peptide 1. Physiol. Rev. 87 (2007) 1409-1439
2. Elliott R.M., Morgan L.M., Tredger J.A., Deacon S., Wright J., and Marks V. Glucagon-like peptide-1 (7-36) amide and glucose-dependent insulinotropic polypeptide secretion in response to nutrient ingestion in man: acute post-prandial and 24-h secretion patterns. J. Endocrinol. 138 (1993) 159-166
3. Nauck M.A., Heimesaat M.M., Orskov C., Holst J.J., Ebert R., and Creutzfeldt W. Preserved incretin activity of glucagon-like peptide 1 [7-36 amide] but not of synthetic human gastric inhibitory polypeptide in patients with type-2 diabetes mellitus. J. Clin. Invest. 91 (1993) 301-307
4. Herrmann C., Goke R., Richter G., Fehmann H.C., Arnold R., and Goke B. Glucagon-like peptide-1 and glucose-dependent insulin-releasing polypeptide plasma levels in response to nutrients. Digestion 56 (1995) 117-126
5. Tolhurst G., Reimann F., and Gribble F.M. Nutritional regulation of glucagon-like peptide-1 secretion. J. Physiol. 587 (2009) 27-32
6. Reimann F., Ward P.S., and Gribble F.M. Signaling mechanisms underlying the release of glucagon-like peptide 1. Diabetes 55 Suppl. 2 (2006) S78-S85
7. Reimann F., Habib A.M., Tolhurst G., Parker H.E., Rogers G.J., and Gribble F.M. Glucose sensing in L cells: a primary cell study. Cell Metab. 8 (2008) 532-539
8. Axelrod D. Total internal reflection fluorescence microscopy. Methods Cell Biol. 30 (1989) 245-270
9. Ohara-Imaizumi M., Nishiwaki C., Kikuta T., Nagai S., Nakamichi Y., and Nagamatsu S. TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells. Biochem. J. 381 (2004) 13-18
10. Lim G.E., and Brubaker P.L. Glucagon-like peptide 1 secretion by the L-cell. Diabetes 55 Suppl. 2 (2006) S70-S77
11. Matsumura K., Miki T., Jhomori T., Gonoi T., and Seino S. Possible role of PEPT1 in gastrointestinal hormone secretion. Biochem. Biophys. Res. Commun. 336 (2005) 1028-1032
12. Wick P.F., Senter R.A., Parsels L.A., Uhler M.D., and Holz R.W. Transient transfection studies of secretion in bovine chromaffin cells and PC12 cells. Generation of kainate-sensitive chromaffin cells. J. Biol. Chem. 268 (1993) 10983-10989
13. Rindi G., Grant S.G., Yiangou Y., Ghatei M.A., Bloom S.R., Bautch V.L., Solcia E., and Polak J.M. Development of neuroendocrine tumors in the gastrointestinal tract of transgenic mice. Heterogeneity of hormone expression. Am. J. Pathol. 136 (1990) 1349-1363
14. Ohara-Imaizumi M., Fujiwara T., Nakamichi Y., Okamura T., Akimoto Y., Kawai J., Matsushima S., Kawakami H., Watanabe T., Akagawa K., and Nagamatsu S. Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis. J. Cell Biol. 177 (2007) 695-705
15. Zenisek D., Steyer J.A., and Almers W. Transport, capture and exocytosis of single synaptic vesicles at active zones. Nature 406 (2000) 849-854
16. Akimoto Y., Hart G.W., Wells L., Vosseller K., Yamamoto K., Munetomo E., Ohara-Imaizumi M., Nishiwaki C., Nagamatsu S., Hirano H., and Kawakami H. Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki rats. Glycobiology 17 (2007) 127-140
17. Eiki J., Saeki K., Nagano N., Iino T., Yonemoto M., Takayenoki-Iino Y., Ito S., Nishimura T., Sato Y., Bamba M., Watanabe H., Sasaki K., Ohyama S., Kanatani A., Nagase T., and Yada T. A selective small molecule glucagon-like peptide-1 secretagogue acting via depolarization-coupled Ca(2+) influx. J. Endocrinol. 201 (2009) 361-367
18. 2+ concentration determines type of fusing insulin granules in pancreatic beta cells. Biochem. Biophys. Res. Commun. 385 (2009) 291-295
19. Mangel A.W., Prpic V., Snow N.D., Basavappa S., Hurst L.J., Sharara A.I., and Liddle R.A. Regulation of cholecystokinin secretion by ATP-sensitive potassium channels. Am. J. Physiol. 267 (1994) G595-G600
20. Pedersen M.G., and Sherman A. Newcomer insulin secretory granules as a highly calcium-sensitive pool. Proc. Natl. Acad. Sci. USA 106 (2009) 7432-7436