Development of a simple and low-cost real-time PCR method for the identification of commonly encountered mycobacteria in a high throughput laboratory

Journal of Applied Microbiology

Volumn 107, Issue 5, 2009, Pages 1433-1439

  Leung, K L ^a   Yip, C W ^a   Cheung, W F ^a   Lo, A C T ^a   Ko, W M ^a   Kam, K M ^a  

^a DEPARTMENT OF HEALTH   (Hong Kong)

Author keywords

Molecular identification; Mycobacteria; Real time PCR

Indexed keywords

COST EFFECTIVENESS; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROBES; THROUGHPUT;

HIGH-THROUGHPUT; LOW-COSTS; MOLECULAR IDENTIFICATION; MYCOBACTERIA; MYCOBACTERIAL; MYCOBACTERIUM TUBERCULOSIS; REAL-TIME PCR; REAL-TIME PCR ASSAY; REAL-TIME PCR METHOD; SIMPLE++;

LABORATORIES;

DNA 16S;

BACTERIUM; DNA; GENE EXPRESSION; IDENTIFICATION METHOD; LABORATORY METHOD; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIUM IDENTIFICATION; COST; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM CHELONEI; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM GORDONAE; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM GORDONAE; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM KANSASII; MYCOBACTERIUM TUBERCULOSIS;

EID: 70349949011     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04324.x     Document Type: Article

References (22)

1. Arnold, L.J., Hammond, P.W., Wiese, W.A. Nelson, N.C. (1989) Assay formats involving acridinium-ester-labeled DNA probes. Clin Chem 35, 1588 1594.
2. Brenner, D.J., Krieg, N.R., Staley, J.T. Garrity, G.M. (2005) Bergey's Manual of Systematic Bacteriology. New York : Springer.
3. Brown-Elliott, B.A. Wallace, R.J. Jr (2002) Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria. Clin Microbiol Rev 15, 716 746.
4. Butler, W.R. Guthertz, L.S. (2001) Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14, 704 726.
5. Clarridge, J.E. III (2004) Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 17, 840 862.
6. Cleary, T.J., Roudel, G., Casillas, O. Miller, N. (2003) Rapid and specific detection of Mycobacterium tuberculosis by using the smart cycler instrument and a specific fluorogenic probe. J Clin Microbiol 41, 4783 4786.
7. Dostal, S., Richter, E. Harmsen, D. (2003) Concise Guide to Mycobacteria and Their Molecular Differentiation. Wurzburg, Germany : Ridom Press.
8. Glassroth, J. (2008) Pulmonary disease due to nontuberculous mycobacteria. Chest 133, 243 251 Erratum in Chest 2008; 133, 1291 20, 198 203.
9. Hanna, B.A., Ebrahimzadeh, A., Elliott, L.B., Morgan, M.A., Novak, S.M., Rusch-Gerdes, S., Acio, M., Dunbar, D.F. et al. (1999) Multicenter evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria. J Clin Microbiol 37, 748 752.
10. Harmsen, D., Rothgänger, J., Singer, C., Albert, J. Frosch, M. (1999) Intuitive hypertext-based molecular identification of micro-organisms. Lancet 353, 291.
11. Kent, P.T. Kubica, G.P. (1985) A Guide for the Level III Laboratory. Atlanta, GA : Centers for Disease Control.
12. Kirschner, P. Bottger, E.C. (2000) Species identification of mycobacteria using rDNA sequencing. In Methods in Molecular Biology, Vol. 101 ed. Parish, T. Stoker, N.G 349 361 Totowa, NJ : Humana Press Inc.
13. Lachnik, J., Ackermann, B., Bohrssen, A., Maass, S., Diephaus, C., Puncken, A., Stermann, M. Bange, F.C. (2002) Rapid-cycle PCR and fluorimetry for detection of mycobacteria. J Clin Microbiol 40, 3364 3373.
14. Lim, S.Y., Kim, B.J., Lee, M.K. Kim, J. (2008) Development of a real-time PCR-based method for rapid differential identification of Mycobacterium species. Lett Appl Microbiol 46, 101 106.
15. Müller, K.D., Schmid, E.N. Kroppenstedt, R.M. (1998) Improved identification of mycobacteria by using the microbial identification system in combination with additional trimethylsulfonium hydroxide pyrolysis. J Clin Microbiol 36, 2477 2480.
16. Padilla, E., González, V., Manterola, J.M., Pérez, A., Quesada, M.D., Gordillo, S. et al. (2004) Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with mycobacterial strains. J Clin Microbiol 42, 3083 3088.
17. Shrestha, N.K., Tuohy, M.J., Hall, G.S., Reischl, U., Gordon, S.M. Procop, G.W. (2003) Detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacterial isolates by real-time PCR. J Clin Microbiol 41, 5121 5126.
18. Springer, B., Stockman, L., Teschner, K., Roberts, G.D. Böttger, E.C. (1996) Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34, 296 303.
19. Tortoli, E. (2003) Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16, 319 354.
20. Tortoli, E., Nanetti, A., Piersimoni, C., Cichero, P., Farina, C., Mucignat, G. et al. (2001) Performance assessment of new multiplex probe assay for identification of mycobacteria. J Clin Microbiol 39, 1079 1084.
21. Williams, K.J., Ling, C.L., Jenkins, C., Gillespie, S.H. McHugh, T.D. (2007) A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory. J Med Microbiol 56, 598 602.
22. Wong, D.A., Yip, P.C., Cheung, D.T. Kam, K.M. (2001) Simple and rational approach to the identification of Mycobacterium tuberculosis, Mycobacterium avium complex species, and other commonly isolated mycobacteria. J Clin Microbiol 39, 3768 3771.