ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, a widespread trait in Burkholderia species, and its growth-promoting effect on tomato plants

Applied and Environmental Microbiology

Volumn 75, Issue 20, 2009, Pages 6581-6590

  Onofre Lemus, Janette ^a   Hernández Lucas, Ismael ^b   Girard, Lourdes ^a   Caballero Mellado, Jesús ^a  

^a INSTITUTO DE CIENCIAS FÍSICAS   (Mexico)

^b INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

1 AMINOCYCLOPROPANE 1 CARBOXYLATES; ACC DEAMINASE; BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; ETHYLENE LEVELS; GENE SEQUENCES; GROWTH-PROMOTING EFFECTS; KNOCKOUT MUTANT; NATURAL CONDITIONS; PHYLOGENETIC ANALYSIS; PLANT GROWTH; RHIZOBACTERIA; TIGHT CLUSTERING; TOMATO PLANTS;

ANIMALS; CARBOXYLATION; ETHYLENE; FRUITS; PLANT LIFE EXTENSION; PLANTS (BOTANY); STRAIN;

GENES;

1 AMINOCYCLOPROPANECARBOXYLIC ACID; 1 AMINOCYCLOPROPANECARBOXYLIC ACID DEAMINASE; 2 OXOBUTYRIC ACID; DEAMINASE; ETHYLENE; UNCLASSIFIED DRUG;

ENZYME ACTIVITY; FRUIT; GENE EXPRESSION; GROWTH RATE; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; PATHOGENICITY; PLANT COMMUNITY; SPECIES DIVERSITY;

ACDS GENE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BURKHOLDERIA; BURKHOLDERIA AMBIFARIA; BURKHOLDERIA CALEDONICA; BURKHOLDERIA CARIBENSIS; BURKHOLDERIA CARYOPHYLLI; BURKHOLDERIA CENOCEPACIA; BURKHOLDERIA CEPACIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA FUNGORUM; BURKHOLDERIA GRAMINIS; BURKHOLDERIA KURURIENSIS; BURKHOLDERIA PHENOLIRUPTRIX; BURKHOLDERIA PHYMATUM; BURKHOLDERIA PHYTOFIRMANS; BURKHOLDERIA SILVATLANTICA; BURKHOLDERIA STABILIS; BURKHOLDERIA TERRICOLA; BURKHOLDERIA TROPICA; BURKHOLDERIA TUBERUM; BURKHOLDERIA UNAMAE; BURKHOLDERIA VIETNAMIENSIS; BURKHOLDERIA XENOVORANS; CONTROLLED STUDY; ENDOPHYTE; ENZYME ACTIVITY; FUSION GENE; GENE CLUSTER; GENE SEQUENCE; GENETIC CONSERVATION; GENETIC TRANSCRIPTION; GUSA GENE; MUTANT; NITROGEN FIXATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLANT GROWTH; PROTEIN EXPRESSION; RHIZOSPHERE; TOMATO;

AMINO ACIDS, CYCLIC; ANIMALS; BASE SEQUENCE; BURKHOLDERIA; CARBON-CARBON LYASES; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; HUMANS; LYCOPERSICON ESCULENTUM; MOLECULAR SEQUENCE DATA; MUTATION; NITROGEN; PHYLOGENY; PLANT GROWTH REGULATORS; SPECIES SPECIFICITY; SYMBIOSIS;

ANIMALIA; BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA UNAMAE; LYCOPERSICON ESCULENTUM; RHIZOBIALES;

EID: 70349932973     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01240-09     Document Type: Article

References (75)

1. Achouak, W., R. Christen, M. Barakat, M.-H. Martel, and T. Heulin. 1999. Burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in Martinique. Int. J. Syst. Bacteriol. 49:787-794.
2. Belimov, A. A., V. I. Safronova, T. A. Sergeyeva, T. N. Egorova, V. A. Matveyeva, V. E. Tsyganov, A. Y. Borisov, I. A. Tikhonovich, C. Kluge, A. Preisfeld, K. J. Dietz, and V. V. Stepanok. 2001. Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase. Can. J. Microbiol. 47:642-652.
3. Blaha, D., C. Pringet-Combaret, M. S. Mirza, and Y. Moënne-Loccoz. 2006. Phylogeny of the 1-aminocyclopropane-1-carboxylic acid deaminase-encoding gene acdS in phytobeneficial and pathogenic proteobacteria and relation with strain biogeography. FEMS Microbiol. Ecol. 56:455-470.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Burkholder, W. 1950. Sour skin, a bacterial rot of onion bulbs. Phytopathology 40:115-118.
6. 2-fixing rhizospheric and endophytic species. Int. J. Syst. Evol. Microbiol. 54:1165-1172.
7. Caballero-Mellado J., J. Onofre-Lemus, P. Estrada-de los Santos, and L. Martínez-Aguilar. 2007. The tomato rhizosphere, an environment rich in nitrogen-fixing Burkholderia species with capabilities of interest for agriculture and bioremediation. Appl. Environ. Microbiol. 73:5308-5319.
8. Campbell, B. G., and J. A. Thomson. 1996. 1-Aminocyclopropane-1- carboxylate deaminase genes from Pseudomonas strains. FEMS Microbiol. Lett. 138:207-210.
9. Cayuela, E., M. T. Estañ, M. Parra, M. Caro, and M. C. Bolarin. 2001. NaCl pre-treatment at the seedling stage enhances fruit yield of tomato plants irrigated with salt water. Plant Soil 230:231-238. (Pubitemid 32368320)
10. Chang, A. C. Y., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
11. Charles, T. C., and T. M. Finan. 1990. Genetic map of Rhizobium meliloti megaplasmid pRmeSU47B. J. Bacteriol. 172:2469-2476.
12. Chen, W.-M., S. M. de Faria, E. James, G. N. Elliott, K.-Y. Lin, J.-H. Chou, S.-Y. Sheu, M. Cnockaert, J. I. Sprent, and P. Vandamme. 2007. Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella. Int. J. Syst. Evol. Microbiol. 57:1055-1059.
13. Chen, W.-M., E. K. James, T. Coenye, J.-H. Chou, E. Barrios, S. M. de Faria, G. N. Elliott, S.-Y. Sheu, J. I. Sprent, and P. Vandamme. 2006. Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int. J. Syst. Evol. Microbiol. 56:1847-1851.
14. Coenye, T., D. Henry, D. P. Speert, and P. Vandamme. 2004. Burkholderia phenoliruptrix sp. nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100. Syst. Appl. Microbiol. 27:623-627.
15. Coenye, T., S. Laevens, A. Willems, M. Ohlén, W. Hannant, J. R. W. Govan, M. Gillis, E. Falsen, and P. Vandamme. 2001. Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int. J. Syst. Evol. Microbiol. 51:1099-1107.
16. Coenye, T., E. Mahenthiralingam, D. Henry, J. J. LiPuma, S. Laevens, M. Gillis, D. P. Speert, and P. Vandamme. 2001. Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int. J. Syst. Evol. Microbiol. 51:1481-1490.
17. Coenye, T., and P. Vandamme. 2003. Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ. Microbiol. 5:719-729.
18. Compant, S., J. Nowak, T. Coenye, C. Clement, and E. A. Barka. 2008. Diversity and occurrence of Burkholderia spp. in the natural environment. FEMS Microbiol. Rev. 32:607-626.
19. Compant, S., B. Reiter, A. Sessitsch, J. Nowak, C. Clément, and E. Ait Barka. 2005. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN. Appl. Environ. Microbiol. 71:1685-1693.
20. Contesto, C., G. Desbrosses, C. Lefoulon, G. Béna, F. Borel, M. Galland, L. Gamet, F. Varoquaux, and B. Touraine. 2008. Effects of rhizobacterial ACC deaminase activity on Arabidopsis indicate that ethylene mediates local root responses to plant growth-promoting rhizobacteria. Plant Sci. 175:178-189.
21. Cuartero, J., M. C. Bolarín, M. J. Asíns, and V. Moreno. 2006. Increasing salt tolerance in the tomato. J. Exp. Bot. 57:1045-1058.
22. Cui, Y., Q. Wang, G. D. Stormo, and J. M. Calvo. 1995. A consensus sequence for binding of Lrp to DNA. J. Bacteriol. 177:4872-4880.
23. Curl, E. A., and B. Truelove. 1986. The rhizosphere. Springer-Verlag, Berlin, Germany.
24. Dalmastri, C., L. Chiarini, C. Cantale, A. Bevivino, and S. Tabacchioni. 1999. Soil type and maize cultivar affect the genetic diversity of maize root-associated Burkholderia cepacia populations. Microb. Ecol. 38:273-284.
25. Dell'Amico, E., L. Cavalca, and V. Andreoni. 2008. Improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biol. Biochem. 40:74-84.
26. Dobbelaere, S., A. Croonenborghs, A. Thys, D. Ptacek, J. Vanderleyden, P. Dutto, C. Labandera-Gonzalez, J. Caballero-Mellado, J. F. Aguirre, Y. Kapulnik, S. Brener, S. Burdman, D. Kadouri, S. Sarig, and Y. Okon. 2001. Responses of agronomically important crops to inoculation with Azospirillum. Aust. J. Plant Physiol. 28:871-879. (Pubitemid 32965061)
27. Dombrecht, B., J. Vanderleyden, and J. Michiels. 2001. Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria. Mol. Plant-Microbe Interact. 14:426-430.
28. Duan, J., K. M. Müller, T. C. Charles, S. Vesely, and B. R. Glick. 2009. 1-Aminocyclopropane-1-carboxylate (ACC) deaminase genes in rhizobia from southern Saskatchewan. Microb. Ecol. 57:423-436.
29. Egener, T., T. Hurek, and B. Reinhold-Hurek. 1999. Endophytic expression of nif genes of Azoarcus sp. strain BH72 in rice roots. Mol. Plant-Microbe Interact. 12:813-819.
30. Elliott, G. N., W. M. Chen, C. Bontemps, J. H. Chou, J. P. W. Young, J. I. Sprent, and E. K. James. 2007. Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum. Ann. Bot. 100:1403-1411.
31. 2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico. Can. J. Microbiol. 48:285-294.
32. Estrada-de los Santos, P., R. Bustillos-Cristales, and J. Caballero-Mellado. 2001. Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl. Environ. Microbiol. 67:2790-2798.
33. Fahraeus, G. 1957. The infection of clover root hairs by nodule bacteria studied by a simple slide technique. J. Gen. Microbiol. 16:379-381.
34. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52:147-154.
35. Finan, T. M., B. Kunkel, G. F. de Vos, and E. R. Signer. 1986. Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J. Bacteriol. 167:66-72.
36. 2-fixing isolates from rice in Vietnam. Int. J. Syst. Bacteriol. 45:274-289.
37. Glick, B. R., C. B. Jacobson, M. M. K. Schwarse, and J. J. Pasternak. 1994. 1-Aminocyclopropane-1-carboxylic acid deaminase mutants of the plant growth promoting rhizobacterium Pseudomonas putida GR 12-2 do not stimulate canola root elongation. Can. J. Microbiol. 40:911-915.
38. Glick, B. R., D. M. Karaturovic, and P. C. Newell. 1995. A novel procedure for rapid isolation of plant growth promoting pseudomonads. Can. J. Microbiol. 41:533-536.
39. Glick, B. R., D. M. Penrose, and J. Li. 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting rhizobacteria. J. Theor. Biol. 190:63-68.
40. Goris, J., W. Dejonghe, E. Falsen, E. De Clerck, B. Geeraertz, A. Willems, E. M. Top, P. Vandamme, and P. De Vos. 2002. Diversity of transconjugants that acquired plasmid pJP4 or pMET1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp. nov. and Burkholderia terricola sp. nov. Syst. Appl. Microbiol. 25:340-352. (Pubitemid 35204119)
41. Goris, J., P. De Vos, J. Caballero-Mellado, J. Park, E. Falsen, J. F. Quensen III, J. M. Tiedje, and P. Vandamme. 2004. Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400T and relatives as Burkholderia xenovorans sp. nov. Int. J. Syst. Evol. Microbiol. 54:1677-1681.
42. Grincko, V. P., and B. R. Glick. 2001. Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria. Plant Physiol. Biochem. 39:11-17.
43. Honma, M., and T. Shimomura. 1978. Metabolism of 1-aminocyclopropane- 1-carboxylic acid. Agric. Biol. Chem. 42:1825-1831.
44. Hontzeas, N., A. O. Richardson, A. Belimov, V. Safranova, M. M. Abu-Omar, and B. R. Glick. 2005. Evidence for horizontal transfer of 1-aminocyclopropane- 1-carboxylate deaminase genes. Appl. Environ. Microbiol. 71:7556-7558.
45. James, E. K., V. M. Reis, F. L. Olivares, J. I. Baldandi, and J. Döbereiner. 1994. Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus. J. Exp. Bot. 45:757-766.
46. Jefferson, R. A., T. A. Kavanagh, and M. W. Bevan. 1987. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901-3907.
47. Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: integrated software for molecular evolutionary genetic analysis and sequence alignment. Brief. Bioinform. 5:150-163.
48. Larkin, M. A., G. Blackshields, N. P. Brown, R. Chenna, P. A. McGettigan, H. McWilliam, F. Valentin, I. M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson, and D. G. Higgins. 2007. ClustalW2 and ClustalX version 2. Bioinformatics 23:2947-2948.
49. Li, J., D. H. Ovakim, T. C. Charles, and B. R. Glick. 2000. An ACC deaminase minus mutant of Enterobacter cloacae UW4 no longer promotes root elongation. Curr. Microbiol. 41:101-105.
50. Ma, W., T. Charles, and B. R. Glick. 2004. Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in Sinorhizobium meliloti increases its ability to nodulate alfalfa. Appl. Environ. Microbiol. 70:5891-5897.
51. Ma, W., F. C. Guinel, and B. R. Glick. 2003. Rhizobium leguminosarum bv. viciae 1-aminocyclopropane-1-carboxylate deaminase promotes nodulation of pea plants. Appl. Environ. Microbiol. 69:4396-4402.
52. Ma, W., S. B. Sebestianova, J. Sebestian, G. I. Burd, F. C. Guinel, and B. R. Glick. 2003. Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp. Antonie van Leeuwenhoek 83:285-291.
53. Martínez-Aguilar, L., R. Díaz, J. J. Peña-Cabriales, P. Estrada-de los Santos, M. F. Dunn, and J. Caballero-Mellado. 2008. Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species. Appl. Environ. Microbiol. 74:4574-4579.
54. Mayak, S., T. Tirosh, and B. R. Glick. 2004. Plant growth promoting bacteria that confer resistance in tomato to salt stress. Plant Physiol. Biochem. 42:565-572.
55. Mayak, S., T. Tirosh, and B. R. Glick. 2004. Plant growth promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci. 166:525-530. (Pubitemid 38214945)
56. Nogales, B., E. R. Moore, E. Llobet-Brossa, R. Rosello-Mora, R. Amann, and K. N. Timmis. 2001. Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil. Appl. Environ. Microbiol. 67:1874-1884.
57. Oke, V., and S. R. Long. 1999. Bacterial genes induced within the nodule during the Rhizobium-legume symbiosis. Mol. Microbiol. 32:837-849.
58. Penrose, D. M., and B. R. Glick. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol. Plant. 118:10-15.
59. Perin, L., L. Martínez-Aguilar, R. Castro-González, P. Estrada-de los Santos, T. Cabellos-Avelar, H. V. Guedes, V. M. Reis, and J. Caballero-Mellado. 2006. Diazotrophic Burkholderia species associated with field-grown maize and sugarcane. Appl. Environ. Microbiol. 72:3103-3110.
60. Perin, L., L. Martínez-Aguilar, G. Paredes, J. I. Baldani, P. Estrada-de los Santos, V. M. Reis, and J. Caballero-Mellado. 2006. Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize. Int. J. Syst. Evol. Microbiol. 56:1931-1937.
61. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127:15-21. (Pubitemid 23140440)
62. Reis, V. M., P. Estrada-de los Santos, S. Tenorio-Salgado, J. Vogel, M. Stoffels, S. Guyon, P. Mavingui, V. L. D. Baldani, M. Schmid, J. I. Baldani, J. Balandreau, A. Hartmann, and J. Caballero-Mellado. 2004. Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium. Int. J. Syst. Evol. Microbiol. 54:2155-2162.
63. Salles, J. F., J. A. van Veen, and J. D. van Elsas. 2004. Multivariate analyses of Burkholderia species in soil: effect of crop and land use history. Appl. Environ. Microbiol. 70:4012-4020.
64. Saravanakumar, D., and R. Samiyappan. 2007. ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants. J. Appl. Microbiol. 102:1283-1292.
65. Sessitsch, A., T. Coenye, A. V. Sturz, P. Vandamme, E. Ait Barka, J. F. Salles, J. D. Van Elsas, D. Faure, B. Reiter, and B. R. Glick. 2005. Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-benefi- cial properties. Int. J. Syst. Evol. Microbiol. 55:1187-1192.
66. Sheehy, R. E., M. Honma, M. Yamada, T. Sasaki, B. Martineau, and W. R. Hiatt. 1991. Isolation, sequence, and expression in Escherichia coli of the Pseudomonas sp. strain ACP gene encoding 1-aminocyclopropane-1-carboxylate deaminase. J. Bacteriol. 173:5260-5265.
67. Uchiumi, T., T. Ohwada, M. Itakura, H. Mitsui, N. Nukui, P. Dawadi, T. Kaneko, S. Tabata, T. Yokoyama, K. Tejima, K. Saeki, H. Omori, M. Hayashi, T. Maekawa, R. Sriprang, Y. Murooka, S. Tajima, K. Simomura, M. Nomura, A. Suzuki, Y. Shimoda, K. Sioya, M. Abe, and K. Minamisawa. 2004. Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome. J. Bacteriol. 186:2439-2448.
68. Vandamme, P., J. Goris, W. M. Chen, P. de Vos, and A. Willems. 2002. Burkholderia tuberum sp. nov. and Burkholderia phymatum sp. nov. nodulate the roots of tropical legumes. Syst. Appl. Microbiol. 25:507-512.
69. Vandamme, P., E. Mahenthiralingam, B. Holmes, T. Coenye, B. Hoste, P. de Vos, D. Henry, and D. P. Speert. 2000. Identification and population structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia genomovar IV). J. Clin. Microbiol. 38:1042-1047.
70. Viallard, V., I. Poirier, B. Cournoyer, J. Haurat, S. Wiebkin, K. Ophel- Keller, and J. Balandreau. 1998. Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of (Pseudomonas) phenazinium, (Pseudomonas) pyrrocinia, and (Pseudomonas) glathei as Burkholderia. Int. J. Syst. Bacteriol. 48:549-563. (Pubitemid 28272413)
71. Vieira, V., and J. Messing. 1991. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100:189-194.
72. Wellburn, A. R. 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers. J. Plant Physiol. 144:307-313.
73. Wilson, K. J., S. G. Huges, and R. A. Jefferson. 1992. The Escherichia coli gus operon, induction and expression of the gus operon in E. coli and the occurrence and use of GUS in other bacteria, p. 7-23. In S. R. Gallagher (ed.), Gus protocols, using the gus gene as a reporter of gene expression, vol.1. Academic Press, San Diego, CA.
74. Yabuuchi, E., Y. Kosako, H. Oyaizu, I. Yano, H. Hotta, Y. Hashimoto, T. Ezaki, and M. Arakawa. 1992. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol. 36:1251-1275.
75. Zhang, H., S. Hanada, T. Shigematsu, K. Shibuya, Y. Kamagata, T. Kanagawa, and R. Kurane. 2000. Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE. Int. J. Syst. Evol. Microbiol. 50:743-749.