Effect of Me2SO on membrane phase behavior and protein denaturation of human pulmonary endothelial cells studied by in situ FTIR spectroscopy

Journal of Biomechanical Engineering

Volumn 131, Issue 7, 2009, Pages

  Spindler, Ralf ^a   Wolkers, Willem F ^a   Glasmacher, Birgit ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Cryopreservation; Fourier transform infrared spectroscopy; Me2SO; Membrane phase behavior; Protein stability

Indexed keywords

CELLULAR ENVIRONMENT; CELLULAR PROTEINS; CONFORMATIONAL DISORDERS; CRYO-PRESERVATION; DESTABILIZING EFFECT; FTIR; IN-SITU FT-IR; ME2SO; MEMBRANE PHASE BEHAVIOR; ONSET TEMPERATURE; PHYSIOLOGICAL TEMPERATURE; PROTEIN DENATURATION; PROTEIN STABILITY; TEMPERATURE RANGE; TEMPERATURE-DEPENDENT CHANGES; TOXIC EFFECT; VIBRATIONAL BANDS;

BIOLOGICAL MATERIALS PRESERVATION; DENATURATION; DIMETHYL SULFOXIDE; ENDOTHELIAL CELLS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; HEATING; MEMBRANES; PHASE BEHAVIOR; PROTEINS; SELF ASSEMBLY;

FOURIER TRANSFORMS;

CELL PROTEIN; DIMETHYL SULFOXIDE; CRYOPROTECTIVE AGENT; PROTEIN;

ARTICLE; CELL CULTURE; CONCENTRATION (PARAMETERS); CRYOPRESERVATION; DOSE RESPONSE; HUMAN; HUMAN CELL; INFRARED SPECTROSCOPY; LUNG ALVEOLUS CELL; PROTEIN DENATURATION; PROTEIN STABILITY; STORAGE TEMPERATURE; CELL MEMBRANE; DRUG EFFECT; ENDOTHELIUM CELL; METABOLISM; METHODOLOGY; PHASE TRANSITION; PULMONARY ARTERY;

CELL MEMBRANE; CELLS, CULTURED; CRYOPROTECTIVE AGENTS; DIMETHYL SULFOXIDE; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIAL CELLS; HUMANS; PHASE TRANSITION; PROTEIN DENATURATION; PROTEINS; PULMONARY ARTERY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 70349904609     PISSN: 01480731     EISSN: 15288951     Source Type: Journal    
DOI: 10.1115/1.3156802     Document Type: Article

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