Expression of P32 protein of goatpox virus in Pichia pastoris and its potential use as a diagnostic antigen in ELISA

Journal of Virological Methods

Volumn 162, Issue 1-2, 2009, Pages 251-257

  Bhanot, V ^a   Balamurugan, V ^a   Bhanuprakash, V ^a   Venkatesan, G ^a   Sen, A ^a   Yadav, V ^a   Yogisharadhya, R ^a   Singh, R K ^a  

^a INDIAN VETERINARY RESEARCH INSTITUTE   (India)

Author keywords

Capripox infections; ELISA; GTPV; P32 protein; Pichia pastoris; Serodiagnosis

Indexed keywords

ACETONE; MACROGOL; METHANOL; MONOCLONAL ANTIBODY; PROTEIN P32; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ANTIBODY SPECIFICITY; ARTICLE; CAPRIPOXVIRUS; CONTROLLED STUDY; ELECTROPORATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FUNGAL STRAIN; FUNGUS CULTURE; GENE AMPLIFICATION; GENE VECTOR; GENETIC SELECTION; GOAT DISEASE; INDIA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRECIPITATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; RECOMBINANT PLASMID; RESTRICTION MAPPING; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SEROEPIDEMIOLOGY; SHEEP DISEASE; TEMPERATURE MEASUREMENT; VIRUS INFECTION; VIRUS NEUTRALIZATION; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CAPRIPOXVIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; PICHIA; POXVIRIDAE INFECTIONS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SHEEP; SHEEP DISEASES; VIRAL PROTEINS;

CAPRA HIRCUS; GOATPOX VIRUS; OVIS ARIES; PICHIA; PICHIA PASTORIS;

EID: 70349828982     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.08.020     Document Type: Article

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