Sequential peptide affinity purification system for the systematic isolation and identification of protein complexes from Escherichia coli.

Methods in molecular biology (Clifton, N.J.)

Volumn 564, Issue , 2009, Pages 373-400

  Babu, Mohan ^a   Butland, Gareth ^a   Pogoutse, Oxana ^a   Li, Joyce ^a   Greenblatt, Jack F ^a   Emili, Andrew ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; MULTIPROTEIN COMPLEX; PEPTIDE FRAGMENT; PROTEINASE; TEV PROTEASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CHEMOLUMINESCENCE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PEPTIDE MAPPING; PROTEOMICS; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; CHEMILUMINESCENT MEASUREMENTS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PROTEOMICS; RECOMBINANT FUSION PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 70349768564     PISSN: 10643745     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-1-60761-157-8_22     Document Type: Article

References (0)