Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants

Biotechnology Letters

Volumn 31, Issue 9, 2009, Pages 1343-1351

  Srinivasan, T ^a   Kumar, K Raja Rajesh ^a   Meur, G ^a   Kirti, P B ^a  

^a UNIVERSITY OF HYDERABAD   (India)

Author keywords

AtNPR1; Methyl viologen; Oxidative stress tolerance; Transgenic tobacco

Indexed keywords

ARABIDOPSIS; ASCORBATE PEROXIDASE; ATNPR1; DISEASE RESISTANCE; EXPRESSOR; GLUCANASE; HETEROLOGOUS EXPRESSION; JASMONATES; METHYL VIOLOGEN; NOVEL FUNCTIONS; OVER-EXPRESSION; PATHOGENESIS-RELATED GENES; SALICYLIC ACIDS; SPODOPTERA LITURA; SUPEROXIDE DISMUTASES; SYSTEMIC ACQUIRED RESISTANCE; THAUMATIN-LIKE PROTEIN; TRANSGENIC PLANTS; TRANSGENIC TOBACCO; TRANSGENIC TOBACCO PLANTS; UP-REGULATION;

ACID RESISTANCE; OXIDATIVE STRESS; OXYGEN; SIMULATED ANNEALING; TOBACCO; ZINC;

PLANTS (BOTANY);

ARABIDOPSIS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; SPODOPTERA LITURA;

ARABIDOPSIS PROTEIN; NPR1 PROTEIN, ARABIDOPSIS; VEGETABLE PROTEIN;

ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; GENE EXPRESSION PROFILING; GENETICS; OXIDATIVE STRESS; PHYSIOLOGICAL STRESS; PHYSIOLOGY; TOBACCO; TRANSGENIC PLANT;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; GENE EXPRESSION PROFILING; OXIDATIVE STRESS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; STRESS, PHYSIOLOGICAL; TOBACCO;

EID: 70349570606     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0022-5     Document Type: Article

References (42)

1. Akiyama T, Pillai AM (2001) Molecular cloning, characterization and in vitro expression of a novel endo-1,3-glucanase up-regulated by ABA and drought stress in rice (Oryza sativa L.). Plant Sci 161:1089-1098
2. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann Rev Plant Biol 55:373-379
3. Arnon DI (1949) Copper enzymes in isolated chloroplasts: poly-phenol oxidase in Beta vulgaris. Plant Physiol 24:1-15
4. Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. J Exp Bot 55:2331-2341
5. Cao H, Li X, Dong X (1998) Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc Natl Acad Sci USA 95:6531-6536
6. Chen MS, Fitzgerald HA, Yadav RC, Canlas PE, Dong X, Ronald PC (2001) Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J 27:101-113
7. Cheng C, Yun KY, Ressom HW, Mohanty B, Bajic VB, Jia Y, Yun SJ, de los Reyes BG (2007) An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice. BMC Genomics 8:175
8. Cheong YH, Chang HS, Gupta R, Wang X, Zhu T, Luan S (2002) Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis. Plant Physiol 129:661-677
9. Despre's C, DeLong C, Glaze S, Liu E, Fobert PR (2000) The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. Plant Cell 12:279-290
10. Dong X (2004) NPR1, all things considered. Curr Opin Plant Biol 7:547-552
11. Doyle JJ, Doyle LJ (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15
12. Friedrich L, Lawton K, Dietrich R, Willits M, Cade R, Ryals J (2001) NIM1 overexpression in Arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides. Mol Plant Microbe Interact 14:1114-1124
13. Fu BY, Xiong JH, Zhu LH, Zhao XQ, Xu HX, Gao YM, Li YS, Xu JL, Li ZK (2007) Identification of functional candidate genes for drought tolerance in rice. Mol Genet Genomics 278:599-609
14. Gorantla M, Babu PR, Reddy LVB, Feltus FA, Paterson AH, Reddy AR (2006) Functional genomics of drought stress response in rice: transcript mapping of annotated unigenes of an indica rice (Oryza sativa L. cv. Nagina 22). Curr Sci 89:496-513
15. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189-198
16. Hong JK, Hwang BK (2005) Induction of enhanced disease resistance and oxidative stress tolerance by overexpression of pepper basic PR-1 gene in Arabidopsis. Physiol Plant 124:267-277
17. Johnson DW, Smith SE, Dobrenz AK (1992) Genetic and phenotypic relationships in response to NaCl at different developmental stages in alfalfa. Theor Appl Genet 83:833-838
18. Kwon SY, Jeong YJ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant Cell Environ 25:873-882
19. Lauchli A, Epstein E (1990) Plant responses to saline and sodic conditions. In: Tanji KK (ed) Agricultural salinity assessment and management. American Society of Civil Engineering, New York, pp 113-137
20. Lee SC, Hwang BK (2006) CASAR8.2A, a pathogen-induced pepper SAR8.2, exhibits an antifungal activity and its overexpression enhances disease resistance and stress tolerance. Plant Mol Biol 61:95-109
21. Lee SC, Kim SH, An SH, Yi SY, Hwang BK (2006) Identification and functional expression of the pepper pathogen-induced gene, CAPIP2, involved in disease resistance and drought and salt stress tolerance. Plant Mol Biol 62:151-164
22. Lee SH, Ahsan N, Lee KW, Kim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH (2007) Simultaneous overexpression of both Cu/Zn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol 164:1626-1638
23. Lin WC, Lu CF, Wu JW, Cheng ML, Lin YM, Yang NS (2004) Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases. Transgenic Res 13:567-581
24. Meur G, Budatha M, Srinivasan T, Kumar KRR, Datta Gupta A, Kirti PB (2008) Overexpression of ArabidopsisNPR1 in tobacco confers enhanced resistance to the early instars of Spodoptera litura. Physiol Plant 133:765-775
25. Mitter R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405-410
26. Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
27. Mylona PV, Polidoros AN, Scandalios JG (2007) Antioxidant gene responses to ROS-generating xenobiotics in developing and germinated scutella of maize. J Exp Bot 58:1-12
28. Niggeweg R, Thurow C, Weigel R, Pfitzner U, Gatz C (2000) Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties. Plant Mol Biol 42:775-788
29. Quilis J, Penas G, Messequer J, Bruqidou C, San Sequndo B (2008) The ArabidopsisAtNPR1 inversely modulates defense responses against fungal, bacterial, or viral pathogens while conferring hypersensitivity to abiotic stresses in transgenic rice. Mol Plant Microbe Interact 21:1215-1231
30. Rajam MV, Chandola N, Goud PS, Singh D, Kashyap V, Choudhary ML, Sihachakr D (2007) Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants. Biol Plant 51:135-141
31. Rayapuram C, Baldwin IT (2007) Increased SA in NPR1-silenced plants antagonizes JA and JA-dependent direct and indirect defenses in herbivore-attacked Nicotiana attenuata in nature. Plant J 52:700-715
32. Sarowar S, Kim YJ, Kim EN, Kim KD, Hwang BK, Islam R, Shin JS (2005a) Overexpression of a pepper basic pathogenesis-related protein-1 gene in tobacco plants enhances resistance to heavy metals and pathogen stress. Plant Cell Rep 24:216-224
33. Sarowar S, Kim EN, Kim YJ, Ok SH, Kim KD, Hwang BK, Shin JS (2005b) Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. Plant Sci 169:55-63
34. Sasaki K, Yuichi O, Hiraga S, Gotoh Y, Seo S, Mitsuhara I, Ito H, Matsui H, Ohashi Y (2007) Characterization of two rice peroxidase promoters that respond to blast fungus-infection. Mol Genet Genomics 278:709-722
35. Sharma P, Kumar S (2005) Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze]. J Biosci 30:231-235
36. Shinozaki K, Dennis ES (2003) Cell signaling and gene regulation. Global analyses of signal transduction and gene expression profiles. Curr Opin Plant Biol 6:405-409
37. Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410-417
38. Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance. Plant Mol Biol 61:6897-6915
39. Spoel SH, Johnson JS, Dong X (2007) Regulation of tradeoffs between plant defences against pathogens with different lifestyles. Proc Natl Acad Sci USA 104:18842-18847
40. Sunkar R, Kapoor A, Zhu JK (2006) Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of mir398 and important for oxidative stress tolerance. Plant Cell 18:2051-2065
41. Zhang YL, Fan WH, Kinkema M, Li X, Dong X (1999) Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci USA 96:6523-6528
42. Zhou JM, Trifa Y, Silva H, Pontier D, Lam E, Shah J, Klessig DF (2000) NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol Plant Microbe Interact 13:191-202