Investigation of the carboligase activity of thiamine diphosphate-dependent enzymes using kinetic modeling and NMR spectroscopy

Journal of Molecular Catalysis B: Enzymatic

Volumn 61, Issue 1-2, 2009, Pages 73-79

  Kokova, Mariya ^a   Zavrel, Michael ^b   Tittmann, Kai ^c   Spiess, Antje C ^b   Pohl, Martina ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Carboligation; Intermediates; Kinetic modeling; NMR spectroscopy; Reaction mechanism; Thiamine diphosphate dependent enzymes

Indexed keywords

CARBOLIGATION; INTERMEDIATES; KINETIC MODELING; NMR SPECTROSCOPY; REACTION MECHANISM; THIAMINE DIPHOSPHATE-DEPENDENT ENZYMES;

ALDEHYDES; BIOLOGICAL MATERIALS PRESERVATION; CATALYSTS; ENZYMES; IGNEOUS ROCKS; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; RATE CONSTANTS; SPECTRUM ANALYSIS;

CONDENSATION REACTIONS;

BENZALDEHYDE LYASE; BENZOIN; BENZOYLFORMATE DECARBOXYLASE; COCARBOXYLASE; LIGASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYMERIZATION; PSEUDOMONAS PUTIDA;

EID: 70349485843     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2009.02.021     Document Type: Article

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