A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma

Biomedical Chromatography

Volumn 23, Issue 9, 2009, Pages 935-939

  Ichinose, Saori ^a,b   Nakamura, Mayumi ^a   Maeda, Mayu ^a   Ikeda, Rie ^a   Wada, Mitsuhiro ^a   Nakazato, Mio ^a   Ohba, Yoshihito ^b   Takamura, Noboru ^a   Maeda, Takahiro ^a   Aoyagi, Kiyoshi ^a   Nakashima, Kenichiro ^a  

^a NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES   (Japan)

^b NAGASAKI INTERNATIONAL UNIVERSITY   (Japan)

Author keywords

Cysteamine; Cysteine; Homocysteine; HPLC FL detection; SBD F

Indexed keywords

AMINO ACIDS; FLUORESCENCE; PLASMA (HUMAN);

CYSTEAMINE; CYSTEINE; FLUORESCENCE METHOD; HOMOCYSTEINES; HPLC-FL DETECTION; HUMAN PLASMAS; MICRO-COLUMNS; SBD-F; SULPHONATES; THIOL DERIVATIVES;

SIGNAL TO NOISE RATIO;

CYSTEINE; HOMOCYSTEINE; MERCAPTAMINE; THIOL DERIVATIVE;

ACCURACY; AMINO ACID ANALYSIS; ARTICLE; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO; TANDEM MASS SPECTROMETRY;

EID: 70349480989     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1205     Document Type: Article

References (22)

1. Bald E, Chwatko G, Gtowacki R and Kusmierek K. Analysis of plasma thiols by high-performance liquid chromatography with ultraviolet detection. Journal of Chromatography A 2004; 1032: 109-115.
2. Benkova B, Lozanov V, Ivanov IP, Todorova A, Milanov I and Mitev V. Determination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl) maleimide. Journal of Chromatography B 2008; 870: 103-108.
3. Boushey CJ, Beresford SAA, Omenn GS and Motulsky AG. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Journal of the American Medical Association 1995; 274: 1049-1057.
4. Fermo I, Arcelloni C, Mazzola G, Angelo AD and Paroni R. Highperformance liquid chromatographic method for measuring total plasma homocysteine levels. Journal of Chromatography B 1998; 719: 31-36.
5. Frick B, Schröcksnadel K, Neurauter G, Wirleitner B, Artner-Dworzak E and Fuchs D. Rapid measurement of total plasma homocysteine by HPLC. Clinical Chimica Acta 2003; 331: 19-23.
6. Garcia AJ and Apitz-Castro R. Plasma total homocysteine quantification: an improvement of the classical high-performance liquid chromatographic method with fluorescence detection of the thiol-SBD derivatives. Journal of Chromatography B 2002; 779: 359-363.
7. Himmelfarb J, McMenamin E and McMonagle E. Plasma aminothiol oxidation in chronic hemodialysis patients. Kidney International 2002; 61: 705-716.
8. Hortin GL, Sullivan P and Csako G. Relationships among plasma homocysteine, cysteine, and albumin concentrations: potential utility of assessing the cysteine/homocysteine ratio. Clinical Chemistry 2001; 47: 1121-1124.
9. Inamoto N, Katsuya T, Kokubo Y, Mannami T, Asai T, Baba S, Ogata J, Tomoike H and Ogihara T. Association of methylenetetrahydrofolate reductase gene polymorphism with carotid atherosclerosis depending on smoking status in Japanese general population. Stroke 2003; 34: 1628-1633.
10. Jacobsen DW, Gatautis VJ, Green R, Robinson K, Savon SR, Secic M, Ji J, Otto JM and Taylor Jr LM. Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: sex differences and correlation with cobalamin and folate concentrations in healthy subjects. Clinical Chemistry 1994; 40: 873-881.
11. Kadota K, Takamura N, Aoyagi K, Yamasaki H, Usa T, Nakazato M, Maeda T, Wada M, Nakashima K, Abe K, Takeshita F and Ozono Y. Circulation Journal 2008; 72: 304-308.
12. Kumar A, John L, Alam MM, Gupta A, Sharma G, Pillai B and Sengupta S. Homocysteine- and cysteine mediated growth defect is not associated with induction of oxidative stress response genes in yeast. Biochemical Journal 2006; 396: 61-69.
13. Mukai Y, Togawa T, Suzuki T, Ohta K and Tanabe S. Determination of homocysteine thiolactone and homocysteine in cell cultures using high-performance liquid chromatography with fluorescence detection. Journal of Chromatography B 2002; 767: 263-268.
14. Nolin TD, McMenamin ME and Himmelfarb J. Simultaneous determination of total homocysteine, cysteine, cysteinylglycine, and glutathione in human plasma by high-performance liquid chromatography: application to studies of oxidative stress. Journal of Chromatography B 2007; 852: 554-561.
15. Özkan Y, Özkan E and Šimšek B. Plasma total homocysteine and cysteine levels as cardiovascular risk factors in coronary heart disease. International Journal of Cardiology 2002; 82: 269-277.
16. Rafii M, Elango R, Courtney-Martin G, House JD, Fisher L and Pencherz PB. High-throughput and simultaneous measurement of homocysteine and cysteine in human plasma and urine by liquid chromatography-electrospray tandem mass spectrometry. Analytical Biochemistry 2007; 371: 71-81.
17. Santa T, Aoyama C, Fukushima T, Imai K and Funatsu T. Suppression of thiol exchange reaction in the determination of reduced form thiols by high-performance liquid chromatography with fluorescence detection after derivatization with fluorogenic benzofurazan reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2, 1,3-benzoxadiazole. Biomedical Chromatography 2006; 20: 656-661.
18. Stachowicz M, Lehmann B, Tibi A, Prognon P, Daurat V and Pradeau D. Determination of total cysteamine in human serum by a high-performance liquid chromatography with fluorescence detection. Journal of Pharmaceutical and Biomedical Analysis 1998; 17: 767-773.
19. Takamura N, Abe Y, Nakazato M, Maeda T, Wada M, Nakashima K, Kusano Y and Aoyagi K. Determinants of plasma homocysteine levels and carotid intima-media thickness in Japanese. Asia Pacific Journal of Clinical Nutrition 2007; 16: 698-703.
20. Wood PL, Khan MA and Moskal JR. Cellular thiol pools are responsible for sequestration of cytotoxic reactive aldehydes: central role of free cysteine and cysteamine. Brain Research 2007; 1158: 158-163.
21. Wronska-Nofer T, Norfer JR, Stetkiewicz J, Wierzbicka M, Bolinska H, Fobker M, Schulte H, Assmann G and Eckardstein A. Evidence for oxidative stress at elevated plasma thiol levels in chronic exposure to carbon disulfide (CS2) and coronary heart disease. Nutrition, Metabolism and Cardiovascular Diseases 2007; 17: 546-553.
22. Yardim-Akaydin S, Özkan Y, Özkan E, Torum M and im ek B. The role of plasma thiol compounds and antioxidant vitamins in patients with cardiovascular diseases. Clinical Chimica Acta 2003; 338: 99-105.