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Ezquerra TA, Garcia-Gutierrez M, Nogales A, Gomez M, editors. Applications of synchrotron light to scattering and diffraction in materials and life sciences. In: Lect. Notes Phys., 776. Berlin Heidelberg: Springer; 2009.
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Ezquerra TA, Garcia-Gutierrez M, Nogales A, Gomez M, editors. Applications of synchrotron light to scattering and diffraction in materials and life sciences. In: Lect. Notes Phys., vol. 776. Berlin Heidelberg: Springer; 2009.
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42449160636
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Dynamics of spontaneous vesicle formation in fluorocarbon and hydrocarbon surfactant mixtures
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With zwitterionic and anionic mixtures, disk-like transient mixed micelles of equimolar composition were observed which evolved by an exponential dynamics and eventually closed to form vesicles within a second. The driving force for this transition is the unstable rim region of the disk-like micelles that generates a line tension which above a critical size overcomes the bending energy and bends the disks to closed objects. In contrast, the corresponding cationic and anionic system did not display any observable intermediate states. The synergy and interaction between surfactant pairs strongly influence the pathways of self-assembly which in turn influences the long term stability of the final structure.
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Weiss T.M., Narayanan T., and Gradzielski M. Dynamics of spontaneous vesicle formation in fluorocarbon and hydrocarbon surfactant mixtures. Langmuir 24 (2008) 3759-3766. With zwitterionic and anionic mixtures, disk-like transient mixed micelles of equimolar composition were observed which evolved by an exponential dynamics and eventually closed to form vesicles within a second. The driving force for this transition is the unstable rim region of the disk-like micelles that generates a line tension which above a critical size overcomes the bending energy and bends the disks to closed objects. In contrast, the corresponding cationic and anionic system did not display any observable intermediate states. The synergy and interaction between surfactant pairs strongly influence the pathways of self-assembly which in turn influences the long term stability of the final structure.
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Langmuir
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Weiss, T.M.1
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A review of microbeam instrumentation and applications to polymer science.
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Nozue Y., Shinohara Y., and Amemiya Y. Application of Microbeam small- and wide-angle X-ray scattering to polymeric material characterization. Polym J 39 (2007) 1221-1237. A review of microbeam instrumentation and applications to polymer science.
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Fundaments of soft condensed matter scattering and diffraction with microfocus techniques
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Springer, Berlin Heidelberg. A review of micro diffraction and scattering instrumental developments and application to polymers and biopolymers.
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Riekel C., Burghammer M., Davies R., Gebhardt R., and Popov D. Fundaments of soft condensed matter scattering and diffraction with microfocus techniques. Lect. Notes Phys. vol. 776 (2009), Springer, Berlin Heidelberg 91-104. A review of micro diffraction and scattering instrumental developments and application to polymers and biopolymers.
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Tsuruta H., and Irving T.C. Experimental approaches for solution X-ray scattering and fiber diffraction. Curr Opin Struct Biol 18 (2008) 601-608
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A review of X-ray intensity fluctuation spectroscopy
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Sutton M. A review of X-ray intensity fluctuation spectroscopy. C R Physique 9 (2008) 657-667
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Gruebel G. X-ray photon correlation spectroscopy at the European X-ray free-electron laser (XFEL) facility. C R Physique 9 (2008) 668-680
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Three-dimensional visualization of a human chromosome using coherent X-ray diffraction
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Coherent X-ray diffraction imaging (CXDI) is a lens less phase-contrast microscopy technique with high image contrast. An unstained human chromosome was imaged using CXDI. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions by electron microscopy. In CXDI, the spatial resolution is often limited by the radiation damage and/or by the statistical precision of the coherent diffraction data at high angles. These limitations may be overcome or lowered dramatically with XFEL.
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Nishino Y., Takahashi Y., Imamoto N., Ishikawa T., and Maeshima K. Three-dimensional visualization of a human chromosome using coherent X-ray diffraction. Phys Rev Lett 102 (2009) 018101. Coherent X-ray diffraction imaging (CXDI) is a lens less phase-contrast microscopy technique with high image contrast. An unstained human chromosome was imaged using CXDI. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions by electron microscopy. In CXDI, the spatial resolution is often limited by the radiation damage and/or by the statistical precision of the coherent diffraction data at high angles. These limitations may be overcome or lowered dramatically with XFEL.
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Phys Rev Lett
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Nishino, Y.1
Takahashi, Y.2
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Maeshima, K.5
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65549154924
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Structural observation and kinetic pathway in the formation of polymeric micelles
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Quantitative modeling revealed that the formation and growth of micelles is well described by a nucleation and growth type process governed by the elemental unimer insertion or expulsion mechanism. The kinetic pathway including the initial aggregation as well as the kinetic stability and the growth rate is controlled by a system specific micellization potential. By appropriately estimating this potential for a given system, e.g., by means of structural investigations or theoretical models, the desired equilibrium or nonequilibrium properties may be predicted.
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Lund R., Willner L., Monkenbusch M., Panine P., Narayanan T., Colmenero J., et al. Structural observation and kinetic pathway in the formation of polymeric micelles. Phys Rev Lett 102 (2009) 188301. Quantitative modeling revealed that the formation and growth of micelles is well described by a nucleation and growth type process governed by the elemental unimer insertion or expulsion mechanism. The kinetic pathway including the initial aggregation as well as the kinetic stability and the growth rate is controlled by a system specific micellization potential. By appropriately estimating this potential for a given system, e.g., by means of structural investigations or theoretical models, the desired equilibrium or nonequilibrium properties may be predicted.
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Phys Rev Lett
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Lund, R.1
Willner, L.2
Monkenbusch, M.3
Panine, P.4
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Colmenero, J.6
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11
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44349098503
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2 phase transition monitored by combined rapid mixing and time-resolved synchrotron SAXS
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2 phase indicated an intermediate phase of a short lifetime (100-400 ms) with a bilayer structure possibly with Pn3 m symmetry.
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2 phase indicated an intermediate phase of a short lifetime (100-400 ms) with a bilayer structure possibly with Pn3 m symmetry.
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PLoS ONE
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Yaghmur, A.1
Laggner, P.2
Sartori, B.3
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X-ray kinematography of phase transformations of three-component lipid mixtures: a time-resolved synchrotron X-ray scattering study using the pressure-jump relaxation technique
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Jeworrek C., Puhse M., and Winter R. X-ray kinematography of phase transformations of three-component lipid mixtures: a time-resolved synchrotron X-ray scattering study using the pressure-jump relaxation technique. Langmuir 24 (2008) 11851-11859
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Jeworrek, C.1
Puhse, M.2
Winter, R.3
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Kinetics of phase transition from lamellar to hexagonally packed cylinders for a triblock copolymer in a selective solvent
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and ibid 9491-9502. The temperature jump induced lamellar to hexagonal transition of the triblock copolymer, polystyrene-b-poly (ethylene-co-butylene)-b-polystyrene (SEBS) in dibutyl phthalate (DBP), a selective solvent for polystyrene (PS), and the hexagonal to body centered cubic (bcc) transition of SEBS in mineral oil, a selective solvent for the middle ethylene-co-butylene (EB) block were studied. The kinetics follow nucleation and growth mechanism for shallow T-jumps and the spinodal decomposition with continuous ordering for deep T-jumps. In the former case, the hexagonally arranged cylinders become modulated along their axis and eventually breaking into spheres to form a bcc lattice and in the latter case, cylinders transform to disordered spheres via a transient bcc state.
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Liu Y., Li M., Bansil R., and Steinhart M. Kinetics of phase transition from lamellar to hexagonally packed cylinders for a triblock copolymer in a selective solvent. Macromolecules 40 (2007) 9482-9490 and ibid 9491-9502. The temperature jump induced lamellar to hexagonal transition of the triblock copolymer, polystyrene-b-poly (ethylene-co-butylene)-b-polystyrene (SEBS) in dibutyl phthalate (DBP), a selective solvent for polystyrene (PS), and the hexagonal to body centered cubic (bcc) transition of SEBS in mineral oil, a selective solvent for the middle ethylene-co-butylene (EB) block were studied. The kinetics follow nucleation and growth mechanism for shallow T-jumps and the spinodal decomposition with continuous ordering for deep T-jumps. In the former case, the hexagonally arranged cylinders become modulated along their axis and eventually breaking into spheres to form a bcc lattice and in the latter case, cylinders transform to disordered spheres via a transient bcc state.
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(2007)
Macromolecules
, vol.40
, pp. 9482-9490
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Liu, Y.1
Li, M.2
Bansil, R.3
Steinhart, M.4
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15
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33947387852
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Scaling behavior of the reorientation kinetics of block copolymers exposed to electric fields
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From the observed time dependence of orientation order parameter, the rotational time constant was deduced which was found to scale with macroscopic viscosity. Slightly above the ODT, the characteristic time constants follow a power law indicating that the reorientation is driven by a decrease in electrostatic energy. The observed exponent suggests an activated process reminiscent of nucleation and growth. The power law dependence of the rate of reorientation derived from computer simulations based on dynamic density functional theory agrees well with the experimental observations.
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Schmidt K., Schoberth H.G., Schubert F., Hänsel F., Fischer F., Weiss T., et al. Scaling behavior of the reorientation kinetics of block copolymers exposed to electric fields. Soft Matt 3 (2007) 448-453. From the observed time dependence of orientation order parameter, the rotational time constant was deduced which was found to scale with macroscopic viscosity. Slightly above the ODT, the characteristic time constants follow a power law indicating that the reorientation is driven by a decrease in electrostatic energy. The observed exponent suggests an activated process reminiscent of nucleation and growth. The power law dependence of the rate of reorientation derived from computer simulations based on dynamic density functional theory agrees well with the experimental observations.
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(2007)
Soft Matt
, vol.3
, pp. 448-453
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Schmidt, K.1
Schoberth, H.G.2
Schubert, F.3
Hänsel, F.4
Fischer, F.5
Weiss, T.6
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16
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38849161677
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Reversible tuning of a block-copolymer nanostructure via electric fields
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Schmidt K., Schoberth H.G., Ruppel M., Zettl H., Hänsel H., Weiss T., et al. Reversible tuning of a block-copolymer nanostructure via electric fields. Nat Mater 7 (2008) 142-145
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Nat Mater
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Schmidt, K.1
Schoberth, H.G.2
Ruppel, M.3
Zettl, H.4
Hänsel, H.5
Weiss, T.6
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18
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42249099208
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Directed motion of proteins along tethered polyelectrolytes
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The time-resolved SAXS intensities were analyzed in terms of the screened Coulomb interaction between the SPB obtained from a reference interaction site integral equation theory and the radial electron density profile of SPB which yielded the amount of adsorbed protein per brush as a function of time.
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Henzler K., Rosenfeldt S., Wittemann A., Harnau L., Finet S., Narayanan T., et al. Directed motion of proteins along tethered polyelectrolytes. Phys Rev Lett 100 (2008) 158301. The time-resolved SAXS intensities were analyzed in terms of the screened Coulomb interaction between the SPB obtained from a reference interaction site integral equation theory and the radial electron density profile of SPB which yielded the amount of adsorbed protein per brush as a function of time.
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Phys Rev Lett
, vol.100
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Henzler, K.1
Rosenfeldt, S.2
Wittemann, A.3
Harnau, L.4
Finet, S.5
Narayanan, T.6
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19
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34547372192
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Probing in situ the nucleation and growth of gold nanoparticles by small-angle X-ray scattering
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The chemical changes prior to the onset of nucleation was probed by means of UV/visible spectroscopy. SAXS revealed that with an alkanoic acid ligand, the nucleation phase was about 1 s followed by growth whose rate is limited by the reaction of the monomers resulting in a particle radius of about 3.7 nm. With an alkylamine, the nucleation rate was increased by an order of magnitude, thus quenching the growth by lack of monomers and yielding a smaller particle radius of about 1 nm.
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Abecassis B., Testard F., Spalla O., and Barboux P. Probing in situ the nucleation and growth of gold nanoparticles by small-angle X-ray scattering. Nano Lett 7 (2007) 1723-1727. The chemical changes prior to the onset of nucleation was probed by means of UV/visible spectroscopy. SAXS revealed that with an alkanoic acid ligand, the nucleation phase was about 1 s followed by growth whose rate is limited by the reaction of the monomers resulting in a particle radius of about 3.7 nm. With an alkylamine, the nucleation rate was increased by an order of magnitude, thus quenching the growth by lack of monomers and yielding a smaller particle radius of about 1 nm.
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(2007)
Nano Lett
, vol.7
, pp. 1723-1727
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Abecassis, B.1
Testard, F.2
Spalla, O.3
Barboux, P.4
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20
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34249930146
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Branching, aggregation, and phase separation during the gelation of tetraethoxysilane
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Gommes C.J., Goderis B., Pirard J.P., and Blacher S. Branching, aggregation, and phase separation during the gelation of tetraethoxysilane. J Non-Cryst Solids 353 (2007) 2495-2499
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Gommes, C.J.1
Goderis, B.2
Pirard, J.P.3
Blacher, S.4
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21
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34250717489
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In situ high-energy synchrotron radiation study of sol-gel nanoparticle formation in supercritical fluids
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Jensen H., Bremholm M., Nielsen R.P., Joensen K.D., Pedersen J.S., Birkedal H., et al. In situ high-energy synchrotron radiation study of sol-gel nanoparticle formation in supercritical fluids. Angew Chem, Int Ed 46 (2007) 1113-1116
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Jensen, H.1
Bremholm, M.2
Nielsen, R.P.3
Joensen, K.D.4
Pedersen, J.S.5
Birkedal, H.6
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41149137733
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Gold nanoparticle superlattice crystallization probed in situ
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Abecassis B., Testard F., and Spalla O. Gold nanoparticle superlattice crystallization probed in situ. Phys Rev Lett 100 (2008) 115504
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23
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54549102230
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Delineating the first few seconds of supramolecular self-assembly of mesostructured titanium oxide thin films through time-resolved small angle X-ray scattering
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Luca V., Bertram W.K., Sizgek G.D., Yang B., and Cookson D. Delineating the first few seconds of supramolecular self-assembly of mesostructured titanium oxide thin films through time-resolved small angle X-ray scattering. Langmuir 24 (2008) 10737-10745
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Luca, V.1
Bertram, W.K.2
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24
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38049187646
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Skeletal muscle resists stretch by rapid actin binding of the second myosin motor domain
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Muscle contraction takes place by the sliding movement of the thick filament (myosin) over the thin filament (actin) within the sarcomere (unit cell). The force is generated by the cyclical interaction between the globular part of myosin and the actin filament. Owing to the symmetry and bipolar arrangement of the force generating molecules (globular or head part of myosin on either side of the sarcomere), the muscle sarcomere acts like an interferometer with X-rays.
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Brunello E., Reconditi M., Elangovan R., Linari M., Sun Y.B., Narayanan T., et al. Skeletal muscle resists stretch by rapid actin binding of the second myosin motor domain. PNAS 104 (2007) 20114. Muscle contraction takes place by the sliding movement of the thick filament (myosin) over the thin filament (actin) within the sarcomere (unit cell). The force is generated by the cyclical interaction between the globular part of myosin and the actin filament. Owing to the symmetry and bipolar arrangement of the force generating molecules (globular or head part of myosin on either side of the sarcomere), the muscle sarcomere acts like an interferometer with X-rays.
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(2007)
PNAS
, vol.104
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Brunello, E.1
Reconditi, M.2
Elangovan, R.3
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Sun, Y.B.5
Narayanan, T.6
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25
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61749088805
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Visualizing solution-phase reaction dynamics with time-resolved X-ray liquidography
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9 photons/pulse. The required statistics in scattered intensity is obtained by averaging over thousands of X-ray pulses with the same time delay from the pump laser flash.
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9 photons/pulse. The required statistics in scattered intensity is obtained by averaging over thousands of X-ray pulses with the same time delay from the pump laser flash.
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Acc Chem Res
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, pp. 356-366
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Ihee, H.1
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Flame aerosol synthesis of smart nanostructured materials
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A comprehensive review of pyrolytic nanoparticle technology, present status, challenges, and opportunities.
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Strobel R., and Pratsinis S.E. Flame aerosol synthesis of smart nanostructured materials. J Mater Chem 17 (2007) 4743-4756. A comprehensive review of pyrolytic nanoparticle technology, present status, challenges, and opportunities.
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Strobel, R.1
Pratsinis, S.E.2
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34250630982
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In-situ study of aggregation of soot particles in an acetylene flame by small angle X-ray scattering
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Sztucki M., Narayanan T., and Beaucage G. In-situ study of aggregation of soot particles in an acetylene flame by small angle X-ray scattering. J Appl Phys 101 (2007) 114304
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Nanomaterial growth dynamics in jet flames
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Unpublished
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Jossen, R., Beaucage, G., Heine, M. C., Narayanan, T., Pratsinis, S. E., Nanomaterial growth dynamics in jet flames, Unpublished.
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High energy X-ray micro-optics
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This article provides an overview of the state-of-the-art in micro and nano-focusing optics and methods for hard X-rays (4-100 keV). Focusing methods fall into three broad categories: reflective (Kirkpatrick-Baez mirror system or multilayers, capillaries and waveguides ), refractive (compound refractive lenses), diffractive (Fresnel zone plates) optics. The fundamental limits in spot size decreases from several tens of nanometers to a few nanometer from reflective to refractive to diffractive optics.
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Snigirev A., and Snigireva I. High energy X-ray micro-optics. C R Physique 9 (2008) 507-516. This article provides an overview of the state-of-the-art in micro and nano-focusing optics and methods for hard X-rays (4-100 keV). Focusing methods fall into three broad categories: reflective (Kirkpatrick-Baez mirror system or multilayers, capillaries and waveguides ), refractive (compound refractive lenses), diffractive (Fresnel zone plates) optics. The fundamental limits in spot size decreases from several tens of nanometers to a few nanometer from reflective to refractive to diffractive optics.
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C R Physique
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Snigirev, A.1
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A new experimental station for simultaneous X-ray microbeam scanning for small- and wide-angle scattering and fluorescence at BESSY II
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Paris O., Li C., Siegel S., Weseloh G., Emmerling F., Riesemeier H., et al. A new experimental station for simultaneous X-ray microbeam scanning for small- and wide-angle scattering and fluorescence at BESSY II. J Appl Crystallogr 40 (2007) s466-s470
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34548507256
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Flight muscle myofibrillogenesis in the pupal stage of Drosophila as examined by X-ray microdiffraction and conventional diffraction
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In the asynchronous flight muscles of higher insects, the lattice planes of contractile filaments are strictly preserved along the length of each myofibril, making the myofibril a millimeter-long giant single multi-protein crystal. To evaluate the extent of long-range myofilament lattice order, end-on myofibrillar microdiffraction patterns were recorded from isolated quick-frozen dorsal longitudinal flight muscle fibres. To assess the extent of development of flight musculature, conventional whole-thorax diffraction patterns were recorded from live pupae which indicated weak hexagonal fluctuations of scattering intensity 15 h after myoblast fusion, and clear hexagonal reflection spots in the following 30 h.
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Iwamoto H., Inoue K., Matsuo T., and Yagi N. Flight muscle myofibrillogenesis in the pupal stage of Drosophila as examined by X-ray microdiffraction and conventional diffraction. Proc R Soc, B 274 (2007) 2297-2305. In the asynchronous flight muscles of higher insects, the lattice planes of contractile filaments are strictly preserved along the length of each myofibril, making the myofibril a millimeter-long giant single multi-protein crystal. To evaluate the extent of long-range myofilament lattice order, end-on myofibrillar microdiffraction patterns were recorded from isolated quick-frozen dorsal longitudinal flight muscle fibres. To assess the extent of development of flight musculature, conventional whole-thorax diffraction patterns were recorded from live pupae which indicated weak hexagonal fluctuations of scattering intensity 15 h after myoblast fusion, and clear hexagonal reflection spots in the following 30 h.
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Proc R Soc, B
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, pp. 2297-2305
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Iwamoto, H.1
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35
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40849100491
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On-axis microbeam wide- and small-angle scattering experiments of a sectioned poly (p-phenylene terephthalamide) fiber
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®) fiber sections of 15 μm length were prepared by laser micro-dissection. On-axis mesh scans by combined SAXS and WAXS using a micron-sized beam confirmed a radial texture of crystalline domains with orientational order differing for skin, central core and intermediate layers which can be correlated with fiber mechanical properties. This study revealed a gradual transition to skin-core region than a sharp interface often assumed in models. A skin-core variation in small-angle scattering demonstrates the evolution of fiber structure due to the heat-treatment process.
-
®) fiber sections of 15 μm length were prepared by laser micro-dissection. On-axis mesh scans by combined SAXS and WAXS using a micron-sized beam confirmed a radial texture of crystalline domains with orientational order differing for skin, central core and intermediate layers which can be correlated with fiber mechanical properties. This study revealed a gradual transition to skin-core region than a sharp interface often assumed in models. A skin-core variation in small-angle scattering demonstrates the evolution of fiber structure due to the heat-treatment process.
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Appl Phys Lett
, vol.92
, pp. 101903
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Davies, R.J.1
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Scanning microbeam X-ray scattering of fibers analyzed by one-dimensional tomography
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Stribeck N., Nöchel U., and Camarillo A.A. Scanning microbeam X-ray scattering of fibers analyzed by one-dimensional tomography. Macromol Chem Phys 209 (2008) 1976-1982
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Scanning X-ray imaging with small-angle scattering contrast
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Gourrier A., Wagermaier W., Burghammer M., Lammie D., Gupta H.S., Fratzl P., et al. Scanning X-ray imaging with small-angle scattering contrast. J Appl Crystallogr 40 (2007) s78-s82
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Ueno S., Nishida T., and Sato K. Synchrotron radiation microbeam X-ray analysis of microstructures and the polymorphic transformation of spherulite crystals of trilaurin. Cryst Growth Des 8 (2008) 751-754
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61549124071
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Microbeam X-ray diffraction analysis of interfacial heterogeneous nucleation of n-hexadecane inside oil-in-water emulsion droplets
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The influence of different types of hydrophobic moieties of surfactants on the crystallization mechanism was investigated using oil (n-hexadecane, C16) within oil-in-water emulsion droplets. The molecular-shape matching between the hydrophobic moiety of surfactant and C16 chains (TW40 of palmitic and TW60 of stearic) induced a rotator phase of C16 at the initial stage of crystallization and the long-chain axes of C16 crystals (with triclinic structure) aligned normal to the membrane interface. These results provided the first evidence of interfacial heterogeneous nucleation caused by molecular interactions at the emulsion membrane, which is thought to be related to the interfacial freezing observed at the oil-water interface with surfactants in bulk.
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Shinohara Y., Takamizawa T., Ueno S., Sato K., Kobayashi I., Nakajima M., et al. Microbeam X-ray diffraction analysis of interfacial heterogeneous nucleation of n-hexadecane inside oil-in-water emulsion droplets. Cryst Growth Des 8 (2008) 3123-3126. The influence of different types of hydrophobic moieties of surfactants on the crystallization mechanism was investigated using oil (n-hexadecane, C16) within oil-in-water emulsion droplets. The molecular-shape matching between the hydrophobic moiety of surfactant and C16 chains (TW40 of palmitic and TW60 of stearic) induced a rotator phase of C16 at the initial stage of crystallization and the long-chain axes of C16 crystals (with triclinic structure) aligned normal to the membrane interface. These results provided the first evidence of interfacial heterogeneous nucleation caused by molecular interactions at the emulsion membrane, which is thought to be related to the interfacial freezing observed at the oil-water interface with surfactants in bulk.
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(2008)
Cryst Growth Des
, vol.8
, pp. 3123-3126
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Shinohara, Y.1
Takamizawa, T.2
Ueno, S.3
Sato, K.4
Kobayashi, I.5
Nakajima, M.6
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40
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34547137371
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High-pressure potato starch granule gelatinization: synchrotron radiation micro-SAXS/WAXS using a diamond anvil cell
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Gebhardt R., Hanfland M., Mezouar M., and Riekel C. High-pressure potato starch granule gelatinization: synchrotron radiation micro-SAXS/WAXS using a diamond anvil cell. Biomacromolecules 8 (2007) 2092-2097
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(2007)
Biomacromolecules
, vol.8
, pp. 2092-2097
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Gebhardt, R.1
Hanfland, M.2
Mezouar, M.3
Riekel, C.4
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41
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35948942711
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Effect of interfacial interaction on the cross-sectional morphology of tobacco mosaic virus using GISAXS
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Lee B., Lo C.T., Thiyagarajan P., Winans R.E., Li X., Niu Z., et al. Effect of interfacial interaction on the cross-sectional morphology of tobacco mosaic virus using GISAXS. Langmuir 23 (2007) 11157-11163
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(2007)
Langmuir
, vol.23
, pp. 11157-11163
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Lee, B.1
Lo, C.T.2
Thiyagarajan, P.3
Winans, R.E.4
Li, X.5
Niu, Z.6
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42
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36349008628
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In situ investigation of the liquid/solid interface of a block copolymer solution under shear stress using microbeam grazing-incidence small-angle X-ray scattering
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170) micelles (13 wt. %) was investigated at different temperatures.
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170) micelles (13 wt. %) was investigated at different temperatures.
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(2007)
Appl Phys Lett
, vol.91
, pp. 213102
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Timmann, A.1
Roth, S.V.2
Fischer, S.3
Förster, S.4
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43
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34548389788
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In situ observation of nanoparticle ordering at the air-water-substrate boundary in colloidal solutions using X-ray nanobeams
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Roth S.V., Autenrieth T., Grübel G., Riekel C., Burghammer M., Hengstler R., et al. In situ observation of nanoparticle ordering at the air-water-substrate boundary in colloidal solutions using X-ray nanobeams. Appl Phys Lett 91 (2007) 091915
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(2007)
Appl Phys Lett
, vol.91
, pp. 091915
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Roth, S.V.1
Autenrieth, T.2
Grübel, G.3
Riekel, C.4
Burghammer, M.5
Hengstler, R.6
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44
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48849107329
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Structural changes of casein micelles in a calcium gradient film
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Gebhardt R., Burghammer M., Riekel C., Roth S.V., and Müller-Buschbaum P. Structural changes of casein micelles in a calcium gradient film. Macromol Biosci 8 (2008) 347-354
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(2008)
Macromol Biosci
, vol.8
, pp. 347-354
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Gebhardt, R.1
Burghammer, M.2
Riekel, C.3
Roth, S.V.4
Müller-Buschbaum, P.5
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45
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44249124816
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Spatial and temporal in situ evolution of the concentration profile during casein micelle ultrafiltration probed by small-angle X-ray scattering
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David C., Pignon F., Narayanan T., Sztucki M., Gesan-Guiziou G., and Magnin A. Spatial and temporal in situ evolution of the concentration profile during casein micelle ultrafiltration probed by small-angle X-ray scattering. Langmuir 24 (2008) 4523-4529
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(2008)
Langmuir
, vol.24
, pp. 4523-4529
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David, C.1
Pignon, F.2
Narayanan, T.3
Sztucki, M.4
Gesan-Guiziou, G.5
Magnin, A.6
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46
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36849076129
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Scanning X-ray microdiffraction of optically manipulated liposomes
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The feasibility was demonstrated using giant multilamellar vesicles of phospholipid (POPE). The lamellar Bragg peak from a cluster of vesicles was recorded.
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Cojoc D., Ferrari E., Garbin V., Di Fabrizio E., Amenitsch H., Rappolt M., et al. Scanning X-ray microdiffraction of optically manipulated liposomes. Appl Phys Lett 91 (2007) 234107. The feasibility was demonstrated using giant multilamellar vesicles of phospholipid (POPE). The lamellar Bragg peak from a cluster of vesicles was recorded.
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(2007)
Appl Phys Lett
, vol.91
, pp. 234107
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Cojoc, D.1
Ferrari, E.2
Garbin, V.3
Di Fabrizio, E.4
Amenitsch, H.5
Rappolt, M.6
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47
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50849088701
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Coherent X-ray diffraction imaging with nanofocused illumination
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Coherent diffraction imaging of single gold particles of size < 100 nm with real space resolution of the order of 5 nm.
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Schroer C.G., Boye P., Feldkamp J.M., Patommel J., Schropp A., Schwab A., et al. Coherent X-ray diffraction imaging with nanofocused illumination. Phys Rev Lett 101 (2008) 090801. Coherent diffraction imaging of single gold particles of size < 100 nm with real space resolution of the order of 5 nm.
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(2008)
Phys Rev Lett
, vol.101
, pp. 090801
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Schroer, C.G.1
Boye, P.2
Feldkamp, J.M.3
Patommel, J.4
Schropp, A.5
Schwab, A.6
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48
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51949090782
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Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering
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The continuous flow setup enabled time-resolved SAXS measurements following photoexcitation of proteins. This approach can elucidate millisecond range conformational changes without signal averaging by photocycle repetition, thus avoiding the sample damage associated with repeat exposures and facilitating measurement of samples with slow photocycles. Notably, this method resolves diffusion-limited association rates, and it is generally applicable to many photoreceptors.
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Lamb J.S., Zoltowski B.D., Pabit S.A., Crane B.R., and Pollack L. Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering. J Am Chem Soc 130 (2008) 12226-12227. The continuous flow setup enabled time-resolved SAXS measurements following photoexcitation of proteins. This approach can elucidate millisecond range conformational changes without signal averaging by photocycle repetition, thus avoiding the sample damage associated with repeat exposures and facilitating measurement of samples with slow photocycles. Notably, this method resolves diffusion-limited association rates, and it is generally applicable to many photoreceptors.
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(2008)
J Am Chem Soc
, vol.130
, pp. 12226-12227
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Lamb, J.S.1
Zoltowski, B.D.2
Pabit, S.A.3
Crane, B.R.4
Pollack, L.5
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49
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33846869485
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Visualization of flow-aligned type I collagen self-assembly in tunable pH gradients
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Köster S., Leach J.B., Struth B., Pfohl T., and Wong J.Y. Visualization of flow-aligned type I collagen self-assembly in tunable pH gradients. Langmuir 23 (2007) 357-359
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(2007)
Langmuir
, vol.23
, pp. 357-359
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Köster, S.1
Leach, J.B.2
Struth, B.3
Pfohl, T.4
Wong, J.Y.5
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50
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38849134103
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Flow at interfaces: a new device for X-ray surface scattering investigations
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Moulin J.F., Roth S.V., and Müller-Buschbaum P. Flow at interfaces: a new device for X-ray surface scattering investigations. Rev Sci Instrum 79 (2008) 015109
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(2008)
Rev Sci Instrum
, vol.79
, pp. 015109
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Moulin, J.F.1
Roth, S.V.2
Müller-Buschbaum, P.3
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51
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34547094809
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Highly packed and oriented DNA mesophases identified using in situ microfluidic X-ray microdiffraction
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Pfohl T., Otten A., Kö ster S., Dootz R., Struth B., and Evans H.M. Highly packed and oriented DNA mesophases identified using in situ microfluidic X-ray microdiffraction. Biomacromolecules 8 (2007) 2167-2172
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(2007)
Biomacromolecules
, vol.8
, pp. 2167-2172
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Pfohl, T.1
Otten, A.2
Kö ster, S.3
Dootz, R.4
Struth, B.5
Evans, H.M.6
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52
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58049215425
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Silk fiber assembly studied by synchrotron radiation SAXS/WAXS and Raman spectroscopy
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Martel A., Burghammer M., Davies R.J., Di Cola E., Vendrely C., and Riekel C. Silk fiber assembly studied by synchrotron radiation SAXS/WAXS and Raman spectroscopy. J Am Chem Soc 130 (2008) 17070-17074
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(2008)
J Am Chem Soc
, vol.130
, pp. 17070-17074
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Martel, A.1
Burghammer, M.2
Davies, R.J.3
Di Cola, E.4
Vendrely, C.5
Riekel, C.6
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53
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51649106422
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Microsecond acquisition of heterogeneous structure in the folding of a TIM barrel protein
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Refolding was initiated by a urea concentration dilution from 6 to 0.6 M corresponding to a final protein concentration of 1 mg/ml. SAXS time resolution of the order of 150 μs was reached but much of the change in the scattering pattern occurred within the intervening time.
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Wu Y., Kondrashkina E., Kayatekin C., Matthews C.R., and Bilsel O. Microsecond acquisition of heterogeneous structure in the folding of a TIM barrel protein. PNAS 105 (2008) 13367-13372. Refolding was initiated by a urea concentration dilution from 6 to 0.6 M corresponding to a final protein concentration of 1 mg/ml. SAXS time resolution of the order of 150 μs was reached but much of the change in the scattering pattern occurred within the intervening time.
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(2008)
PNAS
, vol.105
, pp. 13367-13372
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Wu, Y.1
Kondrashkina, E.2
Kayatekin, C.3
Matthews, C.R.4
Bilsel, O.5
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54
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53349109143
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Colloidal monolayer trapped near a charged wall: a synchrotron X-ray diffraction study
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Satapathy D.K., Bunk O., Jefimovs K., Nyga°rd K., Guo H., Diaz A., et al. Colloidal monolayer trapped near a charged wall: a synchrotron X-ray diffraction study. Phys Rev Lett 101 (2008) 136103
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(2008)
Phys Rev Lett
, vol.101
, pp. 136103
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Satapathy, D.K.1
Bunk, O.2
Jefimovs, K.3
Nygard, K.4
Guo, H.5
Diaz, A.6
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55
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52449085449
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Agglomeration of proteins in acoustically levitated droplets
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The Apoferritin (APO) was used as a model protein, and its aggregation behavior in a levitator was followed from dilute solutions up to the solid form. Different stages of APO agglomeration were observed without solid container walls, which may influence aggregation behavior and also produce parasitic scattering background. Starting with a volume of 5 μl, the high quality scattering curves allowed to extract accurate structure factors of interaction for the concentration range from 5 to 1,200 mg/ml (solid protein).
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Delissen F., Leiterer J., Bienert R., Emmerling F., and Thünemann A.F. Agglomeration of proteins in acoustically levitated droplets. Anal Bioanal Chem 392 (2008) 161-165. The Apoferritin (APO) was used as a model protein, and its aggregation behavior in a levitator was followed from dilute solutions up to the solid form. Different stages of APO agglomeration were observed without solid container walls, which may influence aggregation behavior and also produce parasitic scattering background. Starting with a volume of 5 μl, the high quality scattering curves allowed to extract accurate structure factors of interaction for the concentration range from 5 to 1,200 mg/ml (solid protein).
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(2008)
Anal Bioanal Chem
, vol.392
, pp. 161-165
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Delissen, F.1
Leiterer, J.2
Bienert, R.3
Emmerling, F.4
Thünemann, A.F.5
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56
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60349123107
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Studying macromolecular solutions without wall effects by stroboscopic small-angle X-ray scattering
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Graceffa R., Burghammer M., Davies R.J., Ponchut C., and Riekel C. Studying macromolecular solutions without wall effects by stroboscopic small-angle X-ray scattering. Appl Phys Lett 94 (2009) 062902
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(2009)
Appl Phys Lett
, vol.94
, pp. 062902
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Graceffa, R.1
Burghammer, M.2
Davies, R.J.3
Ponchut, C.4
Riekel, C.5
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