Comment on "the dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles"

Science

Volumn 325, Issue 5947, 2009, Pages

  Granseth, Björn ^a   Odermatt, Benjamin ^b   Royle, Stephen J ^c   Lagnado, Leon ^b  

^a LINKÖPING UNIVERSITY   (Sweden)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^c UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

NANOPARTICLE; NEUROTRANSMITTER; SYNAPTOPHYSIN;

ACIDIFICATION; ENDOCYTOSIS; FLUORESCENCE; HIPPOCAMPUS; MOLECULAR DYNAMICS; MOLECULAR PROBE; NEUROTRANSMISSION; NOTE; POSTSYNAPTIC MEMBRANE; PRESYNAPTIC MEMBRANE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SYNAPSE; SYNAPSE VESICLE;

ACTION POTENTIALS; ANIMALS; ELECTRIC STIMULATION; ENDOCYTOSIS; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; HYDROGEN-ION CONCENTRATION; KINETICS; MEMBRANE FUSION; NEUROTRANSMITTER AGENTS; QUANTUM DOTS; SYNAPSES; SYNAPTIC TRANSMISSION; SYNAPTIC VESICLES;

EID: 70349331677     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1175790     Document Type: Note

References (12)

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9. Dissociated neurons from hippocampi dissected from E18 rats were cocultured with glia and transfected to express a plasmid coding for sypHy using Lipofectamine2000 after 8 days in vitro (DIV). Live fluorescent imaging was carried out at 14 to 21 DIV using an inverted microscope equipped with a Photometries Cascade 512 B camera, frame rate 5 Hz. Action potentials were evoked by field stimulation. For details, see (10).
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12. We are grateful to A. Derevier for assistance with cell culture. B.G. is supported by Linköping Unversity and the Swedish Research Council. L.L. is supported by the Medical Research Council and a Programme Grant from the Wellcome Trust