Direct, real-time monitoring of superoxide generation in isolated mitochondria

Free Radical Research

Volumn 43, Issue 9, 2009, Pages 796-802

  Henderson, James R ^a   Swalwell, Helen ^a   Boulton, Sarahjayne ^a   Manning, Philip ^a   McNeil, Calum J ^a   Birch Machin, Mark A ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Amperometric detection; Complex I; Complex III; Isolated mitochondria; Superoxide

Indexed keywords

CYTOCHROME C; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); SUPEROXIDE; UBIQUINOL CYTOCHROME C REDUCTASE; ANTIMYCIN A1; ENZYME INHIBITOR; GOLD; ROTENONE; SUPEROXIDE DISMUTASE; UNCOUPLING PROTEIN; XANTHINE; XANTHINE OXIDASE;

ARTICLE; CELL FRACTIONATION; CELL ISOLATION; COMPARATIVE STUDY; MITOCHONDRIAL ENERGY TRANSFER; MITOCHONDRION; MONITORING; SENSOR; ANIMAL; CALIBRATION; DRUG ANTAGONISM; DRUG EFFECT; ELECTROCHEMICAL ANALYSIS; ENZYMOLOGY; GENETIC PROCEDURES; HUMAN; INSTRUMENTATION; ION SELECTIVE ELECTRODE; KINETICS; METABOLISM; OXIDATIVE STRESS; STANDARD; TUMOR CELL LINE;

MAMMALIA;

ANIMALS; ANTIMYCIN A; BIOSENSING TECHNIQUES; CALIBRATION; CELL LINE, TUMOR; CYTOCHROMES C; ELECTROCHEMICAL TECHNIQUES; ELECTRON TRANSPORT COMPLEX I; ELECTRON TRANSPORT COMPLEX III; ENZYME INHIBITORS; GOLD; HUMANS; ION-SELECTIVE ELECTRODES; KINETICS; MITOCHONDRIA; OXIDATIVE STRESS; ROTENONE; SUPEROXIDE DISMUTASE; SUPEROXIDES; UNCOUPLING AGENTS; XANTHINE; XANTHINE OXIDASE;

EID: 70349306566     PISSN: 10715762     EISSN: 10292470     Source Type: Journal    
DOI: 10.1080/10715760903062895     Document Type: Article

References (43)

1. Chance B, Mela L. Proton movements in mitochondrial membranes. Nature 1966;212:372-376.
2. Chance B, Mela L. A hydrogen ion concentration gradient in a mitochondrial membrane. Nature 1966;212:369-372.
3. Chance B, Mela L. Hydrogen ion concentration changes in mitochondrial membranes. J Biol Chem 1966;241:4588-4599.
4. Murphy MP. How mitochondria produce reactive oxygen species. Biochem J 2009;417:1-13.
5. Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev 1998;78:547-581.
6. Thannickal VJ, Fanburg BL. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 2000;279:L1005-L1028.
7. Orrenius S, Gogvadze V, Zhivotovsky B. Mitochondrial oxidative stress: implications for cell death. Annu Rev Pharmacol Toxicol 2007;47:143-183. (Pubitemid 46272127)
8. Brand MD, Affourtit C, Esteves TC, Green K, Lambert AJ, Miwa S, Pakay JL, Parker N. Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins. Free Radic Biol Med 2004;37:755-767.
9. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem-Biol Interact 2006;160:1-10
10. Raha S, Robinson BH. Mitochondria, oxygen free radicals, disease and ageing. Trends Biochem Sci 2000;25:502-508.
11. Chang SC, Rodrigues NP, Zurgil N, Henderson JR, Bedioui F, McNeil CJ, Deutsch M. Simultaneous intra-and extracellular superoxide monitoring using an integrated optical and electrochemical sensor system. Biochem Biophys Res Commun 2005;327:979-984. (Pubitemid 40137963)
12. Henderson LM, Chappell JB. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur J Biochem 1993;217:973-980.
13. Aitken GR, Henderson JR, Chang SC, McNeil CJ, Birch-Machin MA. Direct monitoring of UV-induced free radical generation in HaCaT keratinocytes. Clin Exp Dermatol 2007;32:722-727.
14. Cooper JM, Greenough KR, McNeil CJ. Direct electron transfer reactions of immobilized cytochrome c at modified gold electrodes. J Electroanal Chem 1993;347:267-275.
15. Manning P, McNeil CJ, Cooper JM, Hillhouse EW. Direct, real-time sensing of free radical production by activated human glioblastoma cells. Free Radic Biol Med 1998;24:1304-1309.
16. Elks CM, Mariappan N, Haque M, Guggilam A, Majid DS, Francis J. Chronic NF-KB blockade reduces cytosolic and mitochondrial oxidative stress and attenuates renal injury and hypertension in SHR. Am J Physiol 2009;296:F298-F305.
17. Mariappan N, Elks CM, Fink B, Francis J. TNF-induced mitochondrial damage: a link between mitochondrial complex I activity and left ventricular dysfunction. Free Radic Biol Med 2009;46:462-470.
18. Xu X, Arriaga EA. Qualitative determination of superoxide release at both sides of the mitochondrial inner membrane by capillary electrophoretic analysis of the oxidation products of triphenylphosphonium hydroethidine. Free Radic Biol Med 2009;46:905-913.
19. Du G, Mouithys-Mickalad A, Sluse FE. Generation of superoxide anion by mitochondria and impairment of their functions during anoxia and reoxygenation in vitro. Free Radic Biol Med 1998;25:1066-1074.
20. Chang SC, Pereira-Rodrigues N, Henderson JR, Cole A, Bedioui F, McNeil CJ. An electrochemical sensor array system for the direct, simultaneous in vitro monitoring of nitric oxide and superoxide production by cultured cells. Biosens Bioelectron 2005;21:917-922.
21. Datta HK, Manning P, Rathod H, McNeil CJ. Effect of calcitonin, elevated calcium and extracellular matrices on superoxide anion production by rat osteoclasts. Exp Physiol 1995;80:713-719.
22. Manning P, Cookson MR, McNeil CJ, Figlewicz D, Shaw PJ. Superoxide-induced nitric oxide release from cultured glial cells. Brain Res 2001;911:203-210.
23. McNeil CJ, Manning P. Sensor-based measurements of the role and interactions of free radicals in cellular systems. Rev Mol Biotech 2002;82:443-455
24. McNeil CJ, Smith KA, Bellavite P, Bannister JV. Application of the electrochemistry of cytochrome c to the measurement of superoxide radical production. Free Radic Res Commun 1989;7:89-96.
25. Kirby DM, Thorburn DR, Turnbull DM, Taylor RW. Biochemical assays of respiratory chain complex activity. Methods Cell Biol 2007;80:93-119.
26. Muller FL, Liu Y, Van Remmen H. Complex III releases superoxide to both sides of the inner mitochondrial membrane. J Biol Chem 2004;279:49064-49073
27. St-Pierre J, Buckingham JA, Roebuck SJ, Brand MD. Topology of superoxide production from different sites in the mitochondrial electron transport chain. J Biol Chem 2002;277:44784-44790.
28. Miwa S, St-Pierre J, Partridge L, Brand MD. Superoxide and hydrogen peroxide production by Drosophila mitochondria. Free Radic Biol Med 2003;35:938-948.
29. Han D, Williams E, Cadenas E. Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space. Biochem J 2001; 353: 411-416.
30. Birch-Machin M. Assessment of mitochondrial function in vitro and in vivo. In: J Dykens, Y Will, editors. Drug-induced mitochondrial dysfunction. New Jersey: Wiley; 2008. p 383-396.
31. Birch-Machin M, Howell N, Turnbull D. Defects at coupling site II. In: Jones D, Lash L, editors. Methods in toxicology. San Diego: Wiley; 1993. p 324-336.
32. O'Malley Y, Fink BD, Ross NC, Prisinzano TE, Sivitz WI. Reactive oxygen and targeted antioxidant administration in endothelial cell mitochondria. J Biol Chem 2006; 281: 39766-39775.
33. Lambert AJ, Brand MD. Inhibitors of the quinone-binding site allow rapid superoxide production from mitochondrial NADHubiquinone oxidoreductase (complex I). J Biol Chem 2004;279:39414-39420.
34. Ohnishi ST, Ohnishi T, Muranaka S, Fujita H, Kimura H, Uemura K, Yoshida K, Utsumi K. A possible site of superoxide generation in the complex I segment of rat heart mitochondria. J Bioenerg Biomemb 2005;37:1-15.
35. Raijmakers MT, Peters WH, Steegers EA, Poston L. NAD(P)H oxidase associated superoxide production in human placenta from normotensive and pre-eclamptic women. Placenta 2004;25(Suppl A):S85-S89.
36. Doughan AK, Dikalov SI. Mitochondrial redox cycling of mitoquinone leads to superoxide production and cellular apoptosis. Antioxid Redox Sgnal 2007;9:1825-1836.
37. Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem 1995;64:97-112.
38. Okado-Matsumoto A, Fridovich I. Subcellular distribution of superoxide dismutases (SOD) in rat liver: Cu,Zn-SOD in mitochondria. J Biol Chem 2001;276:38388-38393.
39. Meany DL, Thompson L, Arriaga EA. Simultaneously monitoring the superoxide in the mitochondrial matrix and extramitochondrial space by micellar electrokinetic chromatography with laser-induced fluorescence. Anal Chem 2007;79:4588-4594
40. Inarrea P, Moini H, Rettori D, Han D, Martinez J, Garcia I, Fernandez-Vizarra E, Iturralde M, Cadenas E. Redox activation of mitochondrial intermembrane space Cu,Zn-superoxide dismutase. Biochem J 2005;387:203-209.
41. Griendling KK, FitzGerald GA. Oxidative stress and cardiovascular injury: part I: Basic mechanisms and in vivo monitoring of ROS. Circulation 2003;108:1912-1916.
42. Stokes KY, Clanton EC, Russell JM, Ross CR, Granger DN. NAD(P)H oxidase-derived superoxide mediates hypercholes-terolemia-induced leukocyte-endothelial cell adhesion. Circ Res 2001;88:499-505.
43. Taylor RW, Birch-Machin MA, Bartlett K, Lowerson SA, Turnbull DM. The control of mitochondrial oxidations by complex III in rat muscle and liver mitochondria. Implications for our understanding of mitochondrial cytopathies in man. J Biol Chem 1994;269:3523-3528.