Extraction and polyacrylamide gels electrophoresis analysis (SDS-PAGE) of total protein from leaves and roots of Piper tuberculatum;Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE) de proteínas totais de folhas e raízes de Piper tuberculatum

Acta Amazonica

Volumn 39, Issue 2, 2009, Pages 255-260

  Silva, Redinaldo Dos Santos ^a,b   de Souza, Cláudia Regina Batista ^a  

^a FEDERAL UNIVERSITY OF PARÁ   (Brazil)

^b Bolsista PIBIC UFPAR   (Brazil)

Author keywords

Fusarium disease; Fusarium solani f. sp. piperis; Piper nigrum; Piper tuberculatum; Proteomics; SDS PAGE

Indexed keywords

FUSARIUM; FUSARIUM SOLANI; FUSARIUM SOLANI F. SP. PIPERIS; PIPER NIGRUM; PIPER TUBERCULATUM; PIPERACEAE;

EID: 70349303060     PISSN: 00445967     EISSN: None     Source Type: Journal    
DOI: 10.1590/s0044-59672009000200002     Document Type: Article

References (29)

1. Albuquerque, F.C. de; Duarte, M.L.R.; Benchimol, R.L.; Endo, T. 2001. Resistência de piperaceas nativas da Amazônia à infecção causada por Nectria haematococca f. sp. piperis. Acta Amazonica, v.31, n.3, p.341-348.
2. Caetano, P.; Santana, C.G.; Carneiro, R.M.D.; Grossi de Sá, M.F.; Oliveira, J.T.A.; Mehta, A. 2007. Otimização de protocolos para extração de proteínas em raízes de feijão-de-corda (Vigna unguiculata) infectadas com o nematóide Meloidogyne incognita. Boletim de Pesquisa e Desenvolvimento/Embrapa Recursos Genéticos e Biotecnologia, ISSN 1676-1340; 210.
3. Castillejo, M.A.; Amiour, N.; Dumas-Gaudot, E.; Rubiales, D.; Jorrin, J.V. 2004. A proteomic approach to studying plant response to crenate broomrape (Orobanche crenata) in pea (Pisum sativum). Phytochemistry, v.65, n.12, p.1817-1828.
4. Chinnasamy, G.; Rampitsch, C. 2006. Efficient solublization buffers for two-dimensional gel electrophoresis of acidic and basic proteins extracted from wheat seeds. Biochimica et Biophysica Acta, v.1764, n.4, p.641-644.
5. Colditz, F.; Nyamsuren, O.; Niehaus, K.; Eubel, H.; Braun, H.P.; Krajinski, F. 2004. Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches. Plant Molecular Biology, v.55, n.1, p.109-120.
6. Dangl, J.F.; Jones, J.D.G. 2001. Plant pathogens and integrated defence responses to infection. Nature, v.411, p.826-833.
7. De Mot, R.; Vanderleyden, J. 1989. Application of two-dimensional protein analysis for strain fingerprinting and mutant analysis of Azospirillum species. Canadian Journal of Microbiology, v.35, p.960-967.
8. Desimone, M.; Henke, A.; Wagner, E. 1996. Oxidative stress induces partial degradation of the large subunit of ribulose 1,5-biphosphate carboxylase/oxygenase in isolated chloroplasts of barley. Plant Physiology, v.111, p.789-796.
9. Duarte, M.L.R.; Albuquerque, F.C.; Chu, E.Y.; Benchimol, R.L.; Poltronieri, L.S. Manejo integrado da fusariose e da murcha amarela da pimenteira-do-reino. 2002. In: POLTRONIERI, L.S.; TRINDADE, D.R. (Ed.). Manejo integrado das principais doenças de cultivos amazônicos. Belém: Embrapa Amazônia Oriental, p1-16.
10. Galau, G. A.; Wang, H. Y. C.; Wayne H. D. 1991. Sequence of the Gossypium hirsutum D-Genome Alloallele of Legumin A and its mRNA. Plant Physiology, v.3, p.1268-1270.
11. Kim, S.T.; Cho, K.S.; YU, S.; Kim, S.G.; Hong, J.C.; Han, C.D.; Bae, D.W.; Nam, M.H.; Kang, K.Y. 2003. Proteomic analysis of differentially expressed proteins induced by rice blast fungus and elicitor in suspension-cultured rice cells. Proteomics, v.3, n.12, p.2368-2378.
12. Kumar, D.; Klessig, D.F. 2000. Differential induction of tobacco MAP kinases by the defense signals nitric oxid, salicylic, acid, ethylene, and jasmonic acid. Molecular Plant Microbe Interaction, v.13, n.3, p.347-351.
13. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, v.227, p.680-685.
14. Medeiros, R.B.; Ferreira, M.A.S.V.; Dianese, J.C. 2003. Mecanismos de agressão e defesa nas interações planta-patógeno. Brasília: Universidade de Brasília, 209p.
15. Morgano, M. A.; Faria, C. G.; Ferrao, M. F. 2005. Determination of protein in raw coffee for NIR spectroscopy and regression PLS. Ciência Tecnologia de Alimentos, v.25, p.25-31.
16. Nürnberger, T. 1999. Signal perception in plant pathogen defense. Cellular and Molecular Life Science, v.55, p.167-182.
17. Oakley, B.R., Kirsch, D.R., Morris, N.R. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry, v.105, n.2, p.361-3.
18. Osbourn, A. 1996. Preformed antimicrobial compounds and plant defense against fungal attack. The Plant Cell, v.8, p.1821-1831.
19. Park, K.O. 2004. Proteomic studies in plants. Journal of Biochemistry and Molecular Biology, v.37, p.133-138.
20. Patton, W.F. 2002. Detection technologies in proteome analysis. Journal of Chromatography B, v.771, p.3-31.
21. Peck, S.C.; Nuhse, T.S.; Hess, D.; Iglesias, A.; Meins, F.; Boller, T. 2001. Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors. The Plant Cell, v.13, p.1467-1475.
22. Peng-Da MA, Tian-Cheng LU, Xiao-Fu ZHOU, Xiao-Juan ZHU, and Xing-Zhi WANG 2004. Preparation of Polyclonal Antibodies of Rubisco Large and Small Subunits and Their Application in the Functional Analysis of the Genes. Acta Biochimica et Biophysica Sínica, v.36, n.9, p.644-648
23. Poltronieri, M.C.; Lemos, O.F.; Albuquerque, F.C. 1999. Pimenta-do-reino (Piper nigrum L.). In: Embrapa Amazônia Oriental (Ed). Programa de melhoramento genético e de adaptação de espécies vegetais da Amazônia Oriental. Belém: Embrapa Amazônia Oriental. Documentos, v.16, p. 127-137.
24. Sharma, N.; Rahman, M.H.; Strelkov, S.; Thiagarajah, M.; Bansal, V.K.; Kav, N.N.V. 2007. Proteome-level changes in two Brassica napus lines exhibiting differential responses to the fungal pathogen Alternaria brassicae. Plant Science, v.172, p.95-110.
25. Vâlcu, C.M.; Schlink, K. 2006. Efficient extraction of proteins from woody plant samples for two-dimensional electrophoresis. Proteomics, v.6, n.14, p.4166-75.
26. Wang, D.; Eyles, A.; Mandich, D.; Bonello, P. 2006. Systemic aspects of host-pathogen interactions in Austrian pine (Pinus nigra): A proteomics approach. Physiological and Molecular Plant Pathology, v. 68, n.4-6, p.149-157.
27. Weiss, W.; Görg, A. 2008. Sample solublization buffers for two-dimensional electrophoresis. Methods in Molecular Biology, v.424, p.35-42.
28. Xie, H.; Pan, S.; Liu, S.; Ye K.;, Huo, K. 2007. A novel method of protein extraction from perennial Bupleurum root for 2-DE. Electrophoresis, v. 28, p. 871 - 875
29. Yamamoto, E. and Knap, H. T. 2001. Soybean Receptor-like Protein Kinase Genes: